Güneş ÇAKMAK GENÇ, Sevim KARAKAŞ ÇELİK, Mürsel HAZALOĞLU, Müge HARMA, Ahmet DURSUN

Gestasyonel Diyabet ile IL8/CXCL8 rs4073 Gen Polimorfizmi Arasındaki İlişkinin Araştırılması

Amaç: Gestasyonel Diyabet (GDM) ilk defa gebelikte tanı konulan glukoz intoleransıdır. Hem anne hem de bebekte komplikasyon riskini artıran GDM için ileri gebelik yaşı, çok sayıda parite, önceki gebelikte GDM öyküsü, genetik faktörler gibi birçok risk faktörü tanımlanmıştır. Ayrıca bazı inflamatuar mediatörler, kemokinler ve onları kodlayan genlerin polimorfizmlerinin GDM ile ilişkisi gösterilmiştir. Biz bu çalışmada GDM tanısı konulan gebelerle sağlıklı gebelerdeki genotip ve allel frekans dağılımlarını karşılaştırarak, GDM yatkınlığı ile IL8/CXCL8 rs4073 (251A/T) gen polimorfizminin ilişkisini belirlemeyi amaçladık. Gereç ve Yöntemler: Çalışmamıza GDM tanılı 100 gebe ve kontrol grubu olarak 100 sağlıklı gebe dahil edilmiştir. Tüm katılımcılar Zonguldak Bülent Ecevit Üniversitesi Tıp Fakültesi Hastanesi Kadın Hastalıkları ve Doğum Anabilim Dalı’nda takip edilmiştir. IL8/ CXCL8 rs4073 gen polimorfizminin genotiplenmesi, polimeraz zincir reaksiyonu bazlı restriksiyon fragman uzunluk polimorfizmi (PCR-RFLP) yöntemi kullanılarak belirlenmiştir. GDM hastalarında ve kontrollerde her bir gen polimorfizminin genotip sıklığını karşılaştırmak için χ2 testi kullanıldı. Bulgular: Gestasyonel diyabetli gebelerle sağlıklı gebeler arasında rs4073 polimorfizmi genotip ve allel frekans dağılımları açısından istatistiksel olarak anlamlı bir farklılık saptanmamıştır (sırasıyla; p=0,260, p=1,000). Sonuç: Bulgularımız IL8/CXCL8 rs4073 gen polimorfizminin GDM’ye yatkınlıkla ilişkili olmadığını göstermekle birlikte bulgularımızın doğrulanması için farklı etnik grupları içeren geniş örneklem büyüklükleriyle, katılımcıların vücut kütle indeksleri, gebelik öncesi kiloları gibi klinik parametreler de dahil edilerek yapılacak ileri çalışmalara ihtiyaç vardır

Anahtar Kelimeler:

Gestasyonel Diyabet, IL-8, CXCL8, rs4073, gen polimorfizm

Investigation of the Relationship Between Gestational Diabetes Mellitus and IL8/CXCL8 rs4073 Gene Polymorphism

Aim: Gestational Diabetes (GDM) is glucose intolerance that is first diagnosed during pregnancy. Many risk factors such as advanced gestational age, multiparity, history of GDM in a previous pregnancy, and genetic factors have been defined for GDM, which increases the risk of complications in both mother and baby. In addition, some inflammatory mediators, chemokines, and polymorphisms of the genes encoding them are associated with GDM. In this study, we aimed to determine the relationship between GDM susceptibility and IL8/CXCL8 rs4073 (-251A/T) gene polymorphism by comparing the genotype and allele frequency distributions in pregnant women diagnosed with GDM and healthy pregnant women. Material and Methods: One hundred pregnant patients diagnosed with GDM and 100 healthy pregnant as the control group, were included in our study. All participants were followed up in Zonguldak Bülent Ecevit University Medical Faculty Hospital, Department of Obstetrics and Gynecology. Genotyping of the IL8/CXCL8 rs4073 gene polymorphism was determined using the polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method. The χ2 test was used to compare the genotype frequency of each gene polymorphism in GDM patients and controls. Results: There was no statistically significant difference between pregnant women with GDM and healthy pregnant women in terms of rs4073 polymorphism genotype and allele frequency distributions (p=0.260, p=1.000, respectively). Conclusion: Although our findings show that IL8/CXCL8 rs4073 gene polymorphism is not associated with susceptibility to GDM, further studies with large sample sizes including different ethnic groups and clinical parameters of participants such as body mass index and pre-pregnancy weight, are needed to confirm our findings

Keywords:

Gestational diabetes mellitus, IL-8, CXCL8, rs4073, gene polymorphism,

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