Bazı Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae) popülasyonlarında genetik çeşitlilik

Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae), geniş bir konukçu dizisine sahip önemli bir yaprak biti parazitoididir. L. confusus'taki genetik çeşitlilik, Adana ve Hatay illerinde konukçu yaprak biti, konukçu bitki ve yükseklik açısından PCR-RFLP yöntemi ile incelenmiştir. PCR-RFLP yöntemi ile SacI, AccI, HpyCH4III ve EcoP151 enzimlerinin 709 bp mitokondriyal COI gen bölgesinde enzim kesim noktaları saptanmıştır. Gen dizileri diğer ülkelerden (Sırbistan, Çin, Fransa ve İran) olan örnekler karşılaştırıldı ve net sapmaları belirlendi. Balcalı (11) ve Ceyhan (4) örnekleri İran örneğine yakınken, Erzin (5) Sırp ve diğer İran örneğine yakın olarak bulundu. Seyhan'dan Cucumis melo L, Gülek (Pozantı) den Solanum nigrum L., Saimbeyli'den Citrullus lanatus (Tunb.), Ceyhan'dan S. nigrum ve Balcalı'dan Vicia sativa L. örnekleri, PCR-RFLP ile sırasıyla SacI, HpyCH4III, EcoP151 ve EcoP151 ile AccI enzimleri tarafından sırayla kesildiği belirlendi yükseklik, konukçu afit ve bitki türlerine bağlı spesifik genetik çeşitlilik saptanmadı.

Anahtar Kelimeler:

Genetik çeşitlilik, Braconidae, PCR-RFLP, biyolojik kontrol

Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae: Aphidinae) populations

Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae: Aphidiinae) is an important aphid parasitoid that has a wide host range. Genetic diversity in L. confusus was studied by the PCR-RFLP method in terms of host aphid, host plant, and altitude in Adana and Hatay Provinces, Turkey. Restriction sites of SacI, AccI, HpyCH4III, and EcoP151 enzymes on the 709 bp mitochondrial COI gene region was detected by PCR-RFLP method. The sequences were compared with specimens from other countries (Serbia, China, France and Iran) and their net divergence were determined. While the Balcalı (11) and Ceyhan (4) populations were close to the Iran specimen, Erzin (5) was close to the Serbian and other Iran specimens. The specimens of Cucumis melo L. from Seyhan, Solanum nigrum L. from Gülek (Pozantı), Citrullus lanatus (Tunb.) from Saimbeyli, S. nigrum from Ceyhan, and Vicia sativa L. from Balcalı were digested with SacI, HpyCH4III, EcoP151, and both EcoP151 and AccI by PCR-RFLP, respectively. Specific genetic diversity related to altitude, host aphid and plant species was not determined.

Keywords:

Genetic diversity, Braconidae, PCR-RFLP, biological control,

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