Bazı Lysiphlebus confusus (Hymenoptera: Braconidae) popülasyonlarında genetik çeşitlilik

Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae), geniş bir konukçu dizisine sahip önemli bir yaprakbiti parazitoitidir. Bu çalışmada L. confusus'taki genetik çeşitlilik, Adana ve Hatay illerinde yaprakbiti türü, konukçu bitki ve yükseklik açısından PCR-RFLP yöntemi kullanılarak incelenmiştir, böylece L. confusus’un biyolojik mücadelede daha etkin kullanılabilmesi için yeni bulgular elde edilmiştir. PCR-RFLP yöntemi ile SacI, AccI, HpyCH4III ve EcoP151 enzimlerinin 709 bp mitokondriyal COI gen bölgesinde enzim kesim noktaları saptanmıştır. Gen dizileri farklı ülkelerden (Sırbistan, Çin, Fransa ve İran) örnekler ile karşılaştırılmış ve net sapmaları belirlenmiştir. Balcalı (11) ve Ceyhan (4) örnekleri İran örneğine yakın bulunurken, Erzin (5) örneği Sırp ve diğer bir İran örneğine yakın bulunmuştur. Seyhan'dan Cucumis melo L, Gülek (Pozantı)’den Solanum nigrum L., Saimbeyli'den Citrullus lanatus (Tunb.), Ceyhan'dan S. nigrum ve Balcalı'dan Vicia sativa L. örnekleri, PCR-RFLP ile sırasıyla SacI, HpyCH4III, EcoP151 ve EcoP151 ile AccI enzimleri tarafından sırayla kesildiği belirlenmiştir. Yükseklik, konukçu yaprakbiti ve bitki türlerine bağlı spesifik genetik çeşitlilik saptanmamıştır.

Genetic diversity in some Lysiphlebus confusus (Hymenoptera: Braconidae) populations

Lysiphlebus confusus Tremblay & Eady (Hymenoptera: Braconidae:Aphidiinae) is an important aphid parasitoid that has a wide host range. Genetic diversity inL. confusus was studied by the PCR-RFLP method in terms of host aphid, host plant, andaltitude in Adana and Hatay Provinces, Turkey in this research. Thus, new data was obtainedto use more efficient of L. confusus which is common parasitoid in the Mediterranean Regionat biological control. Restriction sites of SacI, AccI, HpyCH4III, and EcoP151 enzymes onthe 709 bp mitochondrial COI gene region was detected by PCR-RFLP method. Thesequences were compared with specimens from other countries (Serbia, China, France andIran) and their net divergence were determined. While the Balcalı (11) and Ceyhan (4)populations were close to the Iran specimen, Erzin (5) was close to the Serbian and other Iranspecimens. The specimens of Cucumis melo L. from Seyhan, Solanum nigrum L. from Gülek(Pozantı), Citrullus lanatus (Tunb.) from Saimbeyli, S. nigrum from Ceyhan, and Vicia sativaL. from Balcalı were digested with SacI, HpyCH4III, EcoP151, and both EcoP151 and AccIby PCR-RFLP, respectively. Specific genetic diversity related to altitude, host aphid andplant species was not determined.

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