Tilapia nilotica'da kadmiyum birkiminin total protein düzeyine etkisi

Bu araştırmada Tilapia nilotica'nn farklı dokularındaki kadmiyum birikim düzeyi ve bu birikimin karaciğer ve böbrek dokularındaki total protein derişimine etkisi incelenmiştir. Belirlenen deney sürelerinin sonunda diseke edilen solungaç, karaciğer ve böbrek dokularındaki kadmiyum derişimi perkin elmer 3100 marka atomik absorbsiyon spektrofotometresi yardımı ile belirlenmiştir. Total protein derişimi belirlenecek olan karaciğer ve böbrek doku örnekleri homojenize edilmişlerdir. Doku homojenatmdaki total protein derişimi Lowry Yöntemi ile belirlenmiştir. Kadmiyum solungaç, karaciğer ve böbrek dokularında yüksek düzeyde birikmiştir. Kadmiyum öncelikle metallothionein (MT) sentez kapasitesi yüksek olan karaciğer dokusunda birikmekle birlikte, deney süresine bağlı olarak en fazla böbrek dokusunda yoğunlaşmıştır. Karaciğer ve böbrek dokularında kadmiyum birikim düzeyine bağlı olarak total protein derişimi artış göstermiştir. Ancak, önceki sürelere göre 60. günde bu dokulardaki kadmiyum ve total protein derişimi bir miktar düşüş göstermiştir.

Effect of cadmium accumulation on total protein levels in tilipia nilotica

The aim of the present study was to determine the accumulation of cadmium in different tissues of Tilapia nilotica and to establish the influence of this accumulation on total protein levels in liver and kidney tissues. At the end of each experimental period; gill, liver and kidney tissues from the animals were dissected and their cadmium accumulation was determined using atomic absorbtion spectrophotometry. Liver and kidney tissues were homogenised to determine total protein levels. The protein contents of the tissue homogenates were determined by the Lowry method. Cadmium accumulation was higher in the gill, liver and kidney tissues. Cadmium primarily accumulated in liver tissue where metalloproteins (MT) are synthetised, while accumulation in kidney tissue exceeded the liver levels with increasing exposure periods. The total protein levels of the liver and kidney tissues rose with increasing cadmium accumulation. The cadmium accumulation and total protein levels decreased, however, on day 60 compared with the previous periods.

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