Gabriel COTOR, Aneta POP, Marian GHITA

The Effect of Ovine Placenta Extract, on Mammogenesis, Lactogenesis and Galactopoiesis in Sheep

The aim of the present study was to evaluate the effect of ovine placenta extract, obtained by affinity chromatography on immobilized wheat germ lectin, on mammogenesis, lactogenesis, and galactopoiesis in pregnant and lactating sheep. Placental extract (100 mg/mL protein concentration) was subcutaneously injected to pregnant sheep in days 1, 4, 7, 10, and 13 of the experiment (totally 10 mL placenta extract/animal) alternatively on the right and left side of the neck. Placental extract induced a more intense development of udder parameters; udder circumference increase was of 16.73%, anteroposterior length increase was of 15.9%, and latero-lateral length of 11.1%. Placental extract administration resulted in 7.92% increase in milk yield. Lactating ewes were monitored for milk production for 6 days before the experiment, after placental extract administration from day 7 to day 14 of the experiment, twice a day, and they presented 14.14% higher milk yield in comparison with the control group, no significant differences in milk lactose and protein content, but an increase in milk fat (6.2%). It may be concluded that the glycoprotein or glycopeptide isolated from sheep placenta by affinity chromatography on wheat germ lectin increased mammogenesis, lactogenesis, and galactopoiesis in sheep.

Anahtar Kelimeler:

mammogenesis, lactogenesis, galactopoiesis, Ovine placenta extract

The Effect of Ovine Placenta Extract, on Mammogenesis, Lactogenesis and Galactopoiesis in Sheep

Keywords:

mammogenesis, lactogenesis, galactopoiesis, Ovine placenta extract,

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Gertler, A., Djiane, F.: Mechanism of ruminant placental lactogen action: Molecular and in vivo studies. Molecul. Genet. Metab., 2002; 75: 189.
Leibovich, H., Gertler, A., Bazer, F., Gootwine, E.: Eff ects of recombinant ovine placental lactogen and recombinant ovine growth hormone on growth of lambs and milk production of ewes. Livest. Product., 2001; Sci. 68:79.
Patel, O.V., Hirako, M., Takahashi, T., Sasaki, N., Domeki, I.: Plasma bovine placental lactogen concentration throughout pregnancy in the cow; relationship to stage of pregnancy, fetal mass, number and postpartum milk yield. Domest. Anim. Endocrinol., 1996; 13: 351-9.
Soares, M.J.: Th e prolactin and growth hormone families: pregnancy-specifi c hormones/cytokines at the maternal-fetal interface. Reproductive Biology and Endocrinology, 2004; 2: 51.
Alvarez-Oxiley, A.V., Sousa, N.M., Hornick, J.L., Touati, K., Van Der Weijden, G.C., Taverne, M.A.M., Beckers, J.F.: Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: determination of fetal concentrations across gestation. Reproduction Fertility and Development, 2007; 19: 877-85.
Leibovich, H., Raver, N., Herman, A., Gregoraszczuk, E.L., Gootwine, E., Gertler, A.: Large-scale preparation of recombinant ovine prolactin and determination of its in vitro and in vivo activity. Protein Expression Purif., 2001; 22: 489- 96.
Constantin, N., Cotruţ, M., Şonea, A.: Fiziologia animalelor domestice. Ed. Coral Sanivet, Bucureşti. 1998.
Cotor, G.: Elemente de fi ziologie a lactaţiei. Ed. Monitor, Bucureşti. 1999.
Min, S.H., Mackenzie, D.D.S., McCutcheon, S.N., Breier, B.H., Gluckman, P.D.: Comparative eff ect of recombinant ovine placental lactogen and bovine growth hormone on galactopoiesis in ewes. J. Dairy Sci., 1997; 80: 640-45.
Pyo, J., Hwang, S.I., Oh, J., Lee, S.J, Kang, S.C., Kim, J.S., Lim, J.: Characterization of a bovine pregnancy associated protein using two-dimensional gel electrophoresis, N-terminal sequencing and mass spectrometry. Proteomics, 2003; 3: 2420- 7.
Forsyth, I.A.: Variation among species in the endocrine control of mammary growth and function; the roles of prolactin, growth hormone and placental lactogen. J. Dairy Sci., 1986; 69: 886.
Martal, J., Chene, N.: Placenta et lactation. Ed. INRA, Toulouse. 1993.
Byatt, J.C., Eppard, P.J., Muniakazi, L., Sorbet, R.H., Veenhuizen, J.J., Curran, D.F., Collier, R.J.: Stimulation of milk yield and feed intake by bovine placental lactogen in the dairy cow. J. Dairy Sci., 1992; 75: 1216-23.
Leibovich, H., Gertler, A., Bazer, F.W., Gootwine, E.: Active immunization of ewes against ovine placental lactogen increases birth weight of lambs and milk production with no adverse eff ect on conception rate. Animal Reproduction Science, 2000; 64: 33-47.