Jingwen ZENG, Danting HE, Xin GUO, Xinna GE, Hanchun YANG

The increase of CD4+CD25+ T cells in the peripheral blood of pigs persistently infected with porcine reproductive and respiratory syndrome virus

Regulatory T lymphocytes (T-regs) may be involved in suppressing the host's immunity, which is important for viral clearance, and may therefore contribute to persistent viral infection. However, nothing is known regarding the situation of porcine T-regs during persistent infection with porcine reproductive and respiratory syndrome virus (PRRSV). This study, which used flow cytometry and real-time PCR, determined that the frequencies of CD4+CD25+ T-cells and porcine Forkhead/winged helix transcription factor (FoxP3) mRNA levels in peripheral blood significantly increased during PRRSV persistence, indicating that CD4+CD25+ T-regs might play an important role in PRRSV persistent infection. This study contributes to a better understanding of the immunological basis of PRRSV persistence.

Anahtar Kelimeler:

Key words: Porcine reproductive and respiratory syndrome virus, PRRSV, persistent infection, CD4+CD25+ T-regs, FoxP3

The increase of CD4+CD25+ T cells in the peripheral blood of pigs persistently infected with porcine reproductive and respiratory syndrome virus

Keywords:

Key words: Porcine reproductive and respiratory syndrome virus, PRRSV, persistent infection, CD4+CD25+ T-regs, FoxP3,

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Neumann, E.J., Kliebenstein, J.B., Johnson, C.D., Mabry, J.W., Bush, E.J., Seitzinger, A.H., Green, A.L., Zimmerman, J.J.: Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J. Am. Vet. Med. Assoc., 2005; 227: 385-392.
Rossow, K.D.: Porcine reproductive and respiratory syndrome. Vet. Pathol., 1998; 35: 1-20.
Cavanaugh, D.: Nidovirales: a new order comprising coronaviridae and arteriviridae. Arch. Virol., 1997; 142: 629- 633.
Cho, J.G., Dee, S.A.: Porcine reproductive and respiratory syndrome virus. Th eriogenology, 2006; 66: 655-662.
Allende, R., Laegreid, W.W., Kutish, G.F., Galeota, J.A., Wills, R.W., Osorio, F.A.: Porcine reproductive and respiratory syndrome virus: description of persistence in individual pigs upon experimental infection. J Virol., 2000; 74: 10834-10837.
Batista, L., Pijoan, C., Dee, S., Olin, M., Molitor, T., Joo, H.S., Xiao, Z., Murtaugh, M.: Virological and immunological responses to porcine reproductive and respiratory syndrome virus in a large population of gilts. Can. J. Vet. Res., 2004; 68: 267-273.
Wills, R.W., Doster, A.R., Galeota, J.A., Sur, J.H., Osorio, F.A.: Duration of infection and proportion of pigs persistently infected with porcine reproductive and respiratory syndrome virus. J. Clin. Microbiol., 2003; 41: 58-62.
Plagemann, P.G., Moennig, V.: Lactate dehydrogenase- elevating virus, equine arteritis virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Adv. Virus Res., 1992; 41: 99-192.
Mateu, E., Diaz, I.: Th e challenge of PRRS immunology. Vet. J., 2008; 177: 345-351.
Drew, T.W.: A review of evidence for immunosuppression due to porcine reproductive and respiratory syndrome virus. Vet. Res., 2000; 31: 27-39.
Diaz, I., Darwich, L., Pappaterra, G., Pujols, J., Mateu, E.: Immune responses of pigs aft er experimental infection with a European strain of porcine reproductive and respiratory syndrome virus. J. Gen. Virol., 2005; 86: 1943-1951.
Lamontagne, L., Pagé, C., Larochelle, R., Magar, R.: Porcine reproductive and respiratory syndrome virus persistence in blood, spleen, lymph nodes, and tonsils of experimentally infected pigs depends on the level of CD8highT cells. Viral. Immunol., 2003; 16: 395-406.
Xiao, Z., Batista, L., Dee, S., Halbur, P., Murtaugh, M.P.: Th e level of virus-specifi c T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of virus load. J. Virol., 2004; 78: 5923- 5933.
Mulupuri, P., Zimmerman, J.J., Hermann, J., Johnson, C.R., Cano, J.P., Yu, W., Dee, S.A., Murtaugh, M.P.: Antigen-specifi c B-cell responses to porcine reproductive and respiratory syndrome virus infection. J. Virol., 2008; 82: 358-370.
Campbell, D.J., Ziegler, S.F.: FOXP3 modifi es the phenotypic and functional properties of regulatory T cells. Nature Rev. Immunol., 2007; 7: 305-310.
Li, S., Gowans, E.J., Chougnet, C., Plebanski, M., Dittmer, U.: Natural regulatory T cells and persistent viral infection. J. Virol., 2008; 82: 21-30.
Yamano, Y., Takenouchi, N., Li, H.C., Tomaru, U., Yao, K., Grant, C.W., Maric, D.A., Jacobson, S.: Virus-induced dysfunction of CD4+CD25+ T cells in patients with HTLV-1- associated neuroimmunological disease. J. Clin. Invest., 2005; 115: 1361-1368.
Robertson, S.J., Messer, R.J., Carmody, A.B., Hasenkrug, K.J.: In vitro suppression of CD8+ T cell function by Friend virus- induced regulatory T cells. J. Immunol., 2006; 176: 3342-3349.
Käser, T., Gerner, W., Hammer, S.E., Patzl, M., Saalmüller, A.: Phenotypic and functional characterization of porcine CD4+CD25high regulatory T cells. Vet. Immunol. Immunopathol., 2008; 122: 153-158.
Käser, T., Gerner, W., Hammer, S.E., Patzl, M., Saalmüller, A.: Detection of Foxp3 protein expression in porcine T lymphocytes. Vet. Immunol. Immunopathol., 2008; 125: 92- 101.
Gao, Z.Q., Guo, X., Yang, H.C.: Genomic characterization of two Chinese isolates of porcine respiratory and reproductive syndrome virus. Arch. Virol., 2004; 149: 1341-1351.
Chung, W.B., Chan, W.H., Chaung, H.C., Lien, Y., Wu, C.C., Huang, Y.L.: Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circo-virus type2 in naturally infected and challenged pigs. J. Virol. Methods, 2005; 124: 11-19.
Lohse, L., Nielsen, J., Eriksen, L.: Temporary CD8+ T-cell depletion in pigs did not exacerbate infection with porcine reproductive and respiratory syndrome virus (PRRSV). Viral Immunol., 2004; 17: 594-603.
Miyara, M., Sakaguchi, S.: Natural regulatory T cells: mechanisms of suppression. Trends Mol. Med., 2007; 13: 108- 116.
Shevach, E.M.: Regulatory T cells in autoimmunity. Annu. Rev. Immunol., 2000; 18: 423-449.
Stoop, J.N., van der Molen, R.G., Baan, C.C., van der Laan, L.J.W., Kuipers, E.J., Kusters, J.G., Janssen, H.L.A.: Regulatory T cells contribute to the impaired immune response in patients with chronic hepatitis B virus infection. Hepatol., 2005; 41: 771-778.
Cabrera, R., Tu, Z., Xu, Y., Firpi, R., Rosen, H.R., Liu, C., Nelson, D.R.: An immunomodulatory role for CD4CD25+ regulatory T lymphocytes in hepatitis C virus infection. Hepatol., 2004; 40: 1062-1071.
Kinter, A.L., Hennessey, M., Bell, A., Kern, S., Lin, Y., Daucher, M., Planta, M., McGlaughlin, M., Jackson, R., Ziegler, S.F., Fauci, A.S.: CD25+CD4+ Regulatory T cells from the peripheral blood of asymptomatic HIV-infected individuals regulate CD4+ and CD8+ HIV-specifi c T cell immune responses in vitro and are associated with favorable clinical markers of disease status. J. Exp. Med., 2004; 200: 331-343.
Andaloussi, A.E., Lesniak, M.S.: An increase in CD4+CD25+FOXP3+ regulatory T cells in tumor-infi ltrating lymphocytes of human glioblastoma multiforme. Neuro Oncol., 2006; 8: 234-243.
Yang, G., Liu, A., Xie, Q., Guo, T.B., Wan, B., Zhou, B., Zhang, J.Z.: Association of CD4+CD25+Foxp3+ regulatory T cells with chronic activity and viral clearance in patients with hepatitis B. Int. Immunol., 2007; 19: 133-140.