The Effect of Different Nitrite Doses and Starter Culture on the Growth of Escherichia coli O157:H7 in Sucuk (Turkish Style Dry Sausage) Processing

The effects of different nitrite doses (100, 150 and 200 ppm NaNO2) and starter culture (Lactobacillus plantarum + Staphylococcus carnosus) on Escherichia coli 0157:H7 were investigated in this study. Sucuk batter was inoculated with Escherichia coli 0157:H7 (106 /g). The count of Escherichia coli 0157:H7 in sucuk samples was determined periodically during ripening/storage by a quantitative method. Lactic acid bacteria (LAB), Enterobacteriaceae counts, pH, nitrite and salt content were also recorded. The number of E. coli 0157:H7, which was 106 kob/g in sucuk batter, decreased during the ripening/storage period at a rate of 0.5 log cycles on the 3rd day, 1 log cycle on the 7th day, approximately 2.5 log cycles on the 14th day, and 4 log cycles on the 21st day. The effects of starter and nitrite on E. coli 0157:H7 count were insignificant. The pH values of sucuk samples decreased dramatically during the first days. The effect of starter culture on the decrease in pH was significant.

The Effect of Different Nitrite Doses and Starter Culture on the Growth of Escherichia coli O157:H7 in Sucuk (Turkish Style Dry Sausage) Processing

The effects of different nitrite doses (100, 150 and 200 ppm NaNO2) and starter culture (Lactobacillus plantarum + Staphylococcus carnosus) on Escherichia coli 0157:H7 were investigated in this study. Sucuk batter was inoculated with Escherichia coli 0157:H7 (106 /g). The count of Escherichia coli 0157:H7 in sucuk samples was determined periodically during ripening/storage by a quantitative method. Lactic acid bacteria (LAB), Enterobacteriaceae counts, pH, nitrite and salt content were also recorded. The number of E. coli 0157:H7, which was 106 kob/g in sucuk batter, decreased during the ripening/storage period at a rate of 0.5 log cycles on the 3rd day, 1 log cycle on the 7th day, approximately 2.5 log cycles on the 14th day, and 4 log cycles on the 21st day. The effects of starter and nitrite on E. coli 0157:H7 count were insignificant. The pH values of sucuk samples decreased dramatically during the first days. The effect of starter culture on the decrease in pH was significant.