The effect of acute fluoride poisoning on nitric oxide and methemoglobin fordmation in the guinea pig

Araştırmada, akut flor zehirlenmesinin nitrik oksit ve methemoglobin oluşumu üzerine etkisinin belirlenmesi amacıyla, 250 mg/kg dozunda sodyum florür yalnız ve flor varlığında verapamil uygulandı ve kontrol grubuna göre kan nitrik oksit (Griess reaksiyonu), kalsiyum düzeyleri ve hemoglobin, methemoglobin, hematokrit değerleri ile alyuvar sayıları belirlendi. Florun uygulanması sonrasında kan nitrik oksit ve methemoglobin düzeyindeki yükselme ile rölatif ilişkili olarak kalsiyum, hemoglobin, hematokrit ve alyuvar değerlerinde azalma belirlendi. Akut flor zehirlenmesinde florun iyonafor etkisi ile kan nitrik oksit düzeyinde şekillenen yükselmenin kalsiyum miktarındaki düşüşle ilişkili olmasından dolayı cNOS kaynaklı olabileceği kanısına varılmıştır.

Kobaylarda akut flor zehirlenmesinin nitrik oksit ve methemoglobin oluşumu üzerine etkisi

To study the effect of acute fluoride poisoning on nitric oxide and methemoglobin formation, 250 mg/kg bw sodium fluoride was applied alone and verapamil was applied together with fluoride. Blood nitric oxide (Griess reaction) and calcium levels; hemoglobin, methemoglobin and hematocrit values; and erythrocyte counts were determined and compared with those of the controls. After the fluoride application it was found that there was a relative relationship between the increase in nitric oxide and methemoglobin levels and the decrease in calcium, hemoglobin and hematocrit levels and erythrocyte count. It was concluded that the increase seen in blood nitric oxide levels as a result of the ionophore effect of fluoride could come from cNOS, as that increase is related to the decrease in calcium amount.

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