Conjugal Transfer and Stability of the Plasmids Determining Exopolysaccharide Production in Lactococcus lactis Strains
A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 x 10-2 - 2.8 x 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 x 10-3 - 4.5 x 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.
Anahtar Kelimeler:
Exopolysaccharide, conjugation, plasmid, Lactococcus lactis
Conjugal Transfer and Stability of the Plasmids Determining Exopolysaccharide Production in Lactococcus lactis Strains
A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 x 10-2 - 2.8 x 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 x 10-3 - 4.5 x 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.
Keywords:
Exopolysaccharide, conjugation, plasmid, Lactococcus lactis,
- ISSN: 1300-0128
- Yayın Aralığı: Yılda 6 Sayı
- Yayıncı: TÜBİTAK
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