JELENA APIC, SLOBODANKA VAKANJAC, IVAN STANCIC, IVAN RADOVIC, STOJA JOTANOVIC, ZDENKO KANACKI, BRANISLAV STANKOVIC

Sow fertility after insemination with varying doses of volume and spermatozoa count

The aim of the present study was to investigate the possibility of increasing boar's reproductive exploitation by using AI doses of doubly reduced volume and sperm count in the intrauterine AI procedure. The experiment was conducted at a commercial pig farm in Serbia in 2014. Classic intracervical insemination (ICI) was performed by using 50 mL or 100 mL volume doses containing 4 Ã 109 or 2 Ã 109 progressively motile spermatozoa. The same volumes and sperm numbers per dose were used with intrauterine insemination (IUI). Each dose combination was used to inseminate 30 sows. Intrauterine insemination with AI doses of reduced volume (50 mL) and sperm count (2 Ã 109) did not produce a statistically significant difference (P < 0.05) in the farrowing rate (76.7%) as compared with 4 Ã 109 spermatozoa in the same volume (83.3%) or with insemination by doses of 100 mL with a 2 Ã 109 (83.3%) or a 4 Ã 109 sperm count (86.7%). The number of live-born piglets (10.82) was larger following IUI using a 50 mL volume dose with a 2 Ã 109 sperm count as compared with ICI with the same AI dose volume and sperm count (9.85). The results show that the use of reduced AI dosages provides an opportunity for the swine industry to considerably exploit the reproductive potential of genetically superior boars.

Anahtar Kelimeler:

Artificial insemination, dose, fertility, number, sow, spermatozoa, volume

Sow fertility after insemination with varying doses of volume and spermatozoa count

Keywords:

Artificial insemination, dose, fertility, number, sow, spermatozoa, volume,

PDF

___

Kommisrud E, Paulenz H, Sehested E, Grevle IS. Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days. Acta Vet Scand 2002; 43: 49–55. 2. Khalifa T, Rekkas C, Samartzi F, Lymberopoulos A, Kousenidis K, Dovenski T. Highlights on artificial insemination (AI) technology in the pigs. Macedonian Veterinary Review 2014; 37: 5–34. 3.
Singleton WL. State of the art in artificial insemination of pigs
in the United States. Theriogenology 2001; 56: 1305–1310. 4.
Stančić B, Grafenau P, Radović I, Petrović M, Božić A. Intensity
of boar sperm utilization in Vojvodina and possibility of its
increase. Contemporary Agriculture 2009; 58: 19–26. 5. StančićB, Zvekić D, Radović I. Božić A, Erdeljan M: Increasing reproductive exploitation of AI boars (a review). In: Proceedings of the 23rd International symposium “New Technologies in Contemporary Animal Production”. Novi Sad, Serbia; 2013. pp. 141–143.
Tummaruk P, Luneheim N, Einarsson S, Dalin AM. Reproductive performance of purebred Swedish Landrace and Swedish Yorkshire sows: II. Effect of mating type, weaning- to-first-service interval and lactation length. Acta Agri Scand A-An 2000; 50: 217–224.
Roseboom JK, Reicks LD, Wilson EM. The reproductive performance and factors affecting on-farm application of low- dose intrauterine deposition of semen in sows. J Anim Sci 2004; 82: 2164–2168.
Mezalira A, Dallanora D, Bernardi LM, Wentz I, Bortolozzo PF. Influence of sperm cell in dose and post-insemination backflow on reproductive performance of intrauterine inseminated sows. Reprod Domest Anim 2005; 40: 1–5.
Dimitrov S, Żmudzki J. Post-cervical insemination of sows with low sperm concentration dose in the commercial pig farm. Bull Vet Inst Pulawy 2009; 53: 225–228.
Stančić B, Radović I, Stančić I, Dragin S, Božić A, Gvozdić D. Fertility of sows after intracervical or intrauterine insemination with different spermatozoa number in reduced volume doses. Acta Vet-Beograd 2010; 60: 257–262.
Stančić I, Stančić B, Dragin S, Radović I, Božić A. Sows fertility after intracervical or postcervical artificial insemination (AI) in worm and cold season. Journal of Microbiology, Biotechnology and Food Sciences 2013; 1592–1601.
Sbardella PE, Ulguim RR, Fontana DL, Ferrari CV, Bernardi ML, Wentz I, Bortolozzo FP. The Post-cervical insemination does not impair the reproductive performance of primiparous sows. Reprod Domest Anim 2014; 49: 59–64.
Zvekić D. Fertility of natural or artificial inseminated sows in the production conditions. MSc, University of Novi Sad, Novi Sad, Serbia, 2003.
Serret CG, Alvarenga FVM, Cória ALP, Dias CP, Corcini DC, Corrêa NM, Deschamps CJ, Bianchi I, Lucia T Jr. Intrauterine artificial insemination of swine with different sperm concentrations, parities, and methods for prediction of ovulation. Anim Reprod 2005; 2: 250–256.
Rozeboom KJ, Troedsson HM, Molitor WT, Crabo GB. The effect of spermatozoa and seminal plasma on leukocyte migration into the uterus of gilts. J Anim Sci 1999; 77: 2201– 2206.
O’Leary S, Jasper JM, Warnes MG, Armstrong TD, Robertson AS. Seminal plasma regulates endometrial cytokine expression, leukocyte recruitment and embryo development in the pig. Reproduction 2004; 128: 237–247.
Steverink DWB, Soede MN, Bouwman GE, Kemp B. Semen backflow after insemination and its effect on fertilization results in sows. Anim Reprod Sci 1998; 54: 109–119.
Garcia JC, Abad M, Kirkwood NR. Effect of sperm numbers and time of insemination relative to ovulation on sow fertility. Anim Reprod Sci 2007; 3–4: 397–401.
Knox V.R. Practicalities and pitfalls of semen evaluation. Advances in Pork Production 2004; 15: 315–321.
Weitze FK. The use of long-term extender in pig AI: a view of the international situation. Pig News and Information 1990; 11: 23–26.
Stančić B, Grafenau P, Pivko J, Gagrčin M. Influence of dilution rate and preservation time on the boar sperm quality. In: Proceedings of the 28th International Conference on Farm Animal Reproduction. Liptovsky Jan, Slovakia; 2002. pp. 110– 112.
Maxwell WMC, de Graaf SP, Ghaoui REH, Evans G. Seminal plasma effects on sperm handling and female fertility. Society for Reproduction and Fertility 2007; 64: 13–38.
Caballero I, Vazquez MJ, García ME, Parrilla I, Roca J, Calvete JJ, Sanz L, Martínez AE. Major proteins of boar seminal plasma as a tool for biotechnological preservation of spermatozoa. Theriogenology 2008; 70: 1352–1355.
Stančić I, Dragin S, Stančić B, Harvey R, Božić A, Anderson R. Effects of breed, spermatozoa concentration, and storage on progressive motility of extended boar semen. Journal of Microbiology, Biotechnology and Food Sciences 2012; 3: 287– 295.
Strzežek J, Wysocki P, Kuklinska M. Proteomics of boar seminal plasma – current studies and possibility of their application in biotechnology of animal reproduction. Reprod Biol 2005; 5: 279–290.
Nasrin SJ, Calogero S. Seminal plasma: An essential attribute to spermatozoa (a review). J Androl 2012; 33: 536–451.
Hunter, RHF. Sperm transport and reservoirs in the pig oviduct in relation to the time of ovulation. J Reprod Fertil 1981; 63: 109–115.
O’Leary S, Robertson AS, David T, Armstrong TD. The influence of seminal plasma on ovarian function in pigs—a novel inflammatory mechanism? J Reprod Immunol 2002; 57: 225–238.
Waberski D, Döhring A, Ardon F, Ritter N, Zerbe H, Schubert HJ, Hewicker-Trautwen M, Witze FK, Hunter RHF. Physiological routes from intra-uterine seminal contents to advancement of ovulation. Acta Vet Scand 2006; 48: 1–8.
Rodríguez-Martínez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ. Seminal plasma proteins: what role do they play? Am J Reprod Immunol 2011; 66: 11–22.