Malgorzata Ras NORYNSKA, Rajmund SOKOL, Andrzej Krzysztof SIWICKI

Selected blood immunological and biochemical parameters in horses infected with Cyathostominae before and after ivermectin treatment

The objective of this study was to identify selected blood immunological and biochemical parameters in horses infected with Cyathostominae before and after treatment with ivermectin. Fecal samples were collected before ivermectin treatment and on days 1, 2, 3, 4, 10, 20, 30, 40, 50, 60, and 75 after drug administration. Blood was sampled on the same days to determine levels of total protein, g-globulin, ceruloplasmin, and lysozyme activity. Eggs and dead parasites were excreted until day 3 postivermectin treatment. The reappearance of eggs was observed in 1 horse on day 50, and on day 75 in the remaining animals. Before treatment, total protein levels were measured at 53.2 g/L on average, and a decrease was noted until day 3 after treatment, with minor fluctuations on the following days. The highest g-globulin concentrations of 14.1 g/L were reported on day 10 after treatment. Ceruloplasmin levels were determined at 74 IU before treatment, and a significant increase to 106 IU was observed on day 40. Lysozyme activity before ivermectin treatment reached 2.77 mL/L on average, and a significant 2-fold increase to 6.85 mg/L was reported on day 75.

Anahtar Kelimeler:

Horses, Cyathostominae, ivermectin, immunology

Selected blood immunological and biochemical parameters in horses infected with Cyathostominae before and after ivermectin treatment

Keywords:

Horses, Cyathostominae, ivermectin, immunology,

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Gawor J. Pasożyty wewnętrzne u koni- epidemiologia i zwalczanie. Magazyn Wet 2009; 18: 1068–1074 (article in Polish with an English abstract).
Lyons ET, Tolliver SC, Collins SS. Probable reason why small strongyle EPG counts are returning “early” after ivermectin treatment of horses on a farm in central Kentucky. Parasitol Res 2009; 104: 569–574.
Mair TS. Outbreak of larval cyathostomiasis among a group of yearlings and 2-year-old horses. Vet Rec 1994; 135: 598–600.
Murphy D, Love S. The pathogenic effects of experimental cyathostome infections in ponies. Vet Parasitol 1997; 70: 99–110.
Love S, Murphy D, Mellor D. Pathogenicity of cyathostome infection. Vet Parasitol 1999; 85: 113–122.
Abbot JB, Mellor DJ, Love S. Assessment of serum protein electrophoresis for monitoring therapy of naturally acquired equine cyathostomin infections. Vet Parasitol 2007; 147: 110– 117.
Siwicki AK, Anderson DP. Nonspecific defence mechanisms assay in fish II: Potential killing activity of neutrophils and manocytes, lysozome activity in serum and organs and total immunoglobulin (Ig) level in serum. Fish Disease Diagnosis and Preventions Methods. FAO-Project GCP/INT/526/JPN, IFI Olsztyn: 1993; 105–112.
Nazifi S, Razavi SM, Reiszadeh M, Esmailnezhad Z, Ansari- Lari M. Diagnostic values of acute phase proteins in Iranian indigenous cattle infected with Theileria annulata. Vet Arhiv 2010; 80: 205–214.
Nazifi S, Razavi SM, Esmailnejad Z, Gheisari H. Study on acute phase proteins (haptoglobin, serum amyloid A, fibrinogen, and ceruloplasmin) changes and their diagnostic values in bovine tropical theileriosis. Parasitol Res 2009; 105; 41–46.
Whitlock HV. Some modifications of the McMaster helminth egg-counting technique and apparatus. J Council Sci Ind Res 1948; 21: 277–297.
Ziomko I, Cencek T. Inwazje pasożytnicze zwierząt gospodarskich- wybrane metody diagnostyczne. Drukarnia Piotra Włodarskiego, Warszawa. 1999 (book in Polish).
Rice EW, Wagman E, Takenaka Y. Ceruloplasmin assay in serum: standardization of ceruloplasmin activity in terms of international enzyme units. Diagnostic Laboratory 1986; 12: 39–53.
Matthews JB, Hodgkinson JE, Dowdall SMJ, Proudman CJ. Recent developments in research into the Cyathostominae and Anoplocephala perfoliata. Vet Rec 2004; 35: 371–381.
Giles CJ, Urquhart KA, Longstaffe JA. Larvae cyathostomiasis (immature trichonema-induced enteropathy): A report of 15 clinical cases. Equine Vet J 1985; 17: 196–201.
Anderson N, Armour J, Jarrett WFH, Jennings FW, Ritchie JSD, Urquhart GM. A field study of parasitic gastroenteritis in cattle. Vet Rec 1965; 77: 1196–1204.
Demeulenaere D, Vercruysse J, Dorny P, Claerebout E. Comparative studies of ivermectin and moxidectin in the control of naturally acquired cyathostome infections in horses. Vet Rec 1997; 141: 383–386.