Zinka MAKSIMOVIC, Christian DE LA FE, Maid RIFATBEGOVIC

Presence of mycoplasmas in the respiratory system of small ruminants managed under an extensive production system

The objective of this study was to investigate the presence of mycoplasmas in the respiratory system of sheep and goat populations managed under extensive production systems. Specific culture and polymerase chain reaction techniques were performed on a total of 88 lung tissue samples and 142 nasal swabs obtained from multiple herds in Bosnia and Herzegovina. A total of 29 samples (12.6%) were found to be positive for mycoplasmas. Mycoplasma ovipneumoniae was detected in the nasal swabs and lungs of affected sheep (n = 19) and in the lungs of asymptomatic goat kids (n = 2), while M. arginini (n = 5) and M. bovigenitalium (n = 1) were found in the goats' respiratory tracts. These data confirm the importance of M. ovipneumoniae in sheep raised under an extensive production system. This report also provides the first evidence of M. bovigenitalium isolation from the respiratory tract of goats.

Anahtar Kelimeler:

Key words: Mycoplasma ovipneumoniae, Mycoplasma bovigenitalium, goat, sheep, extensive production

Presence of mycoplasmas in the respiratory system of small ruminants managed under an extensive production system

Keywords:

Key words: Mycoplasma ovipneumoniae, Mycoplasma bovigenitalium, goat, sheep, extensive production,

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Thonney, M.L., Smith, M.C., Mateescu R.G., Heuer, C.: Vaccination of ewes and lambs against parainfluenza 3 to prevent lamb pneumonia. Small. Ruminant Res., 2008; 74: 30–
Nicholas, R., Ayling, R., McAuliffe, L.: Respiratory diseases of small ruminants. In: Nicholas, R., Ayling, R., Mcauliffe, L., Eds. Mycoplasma Diseases of Ruminants. CABI, Wallingford, UK; 2008: 171–179.
Nicholas, R.A.J., Ayling, R.D., Loria, G.R.: Ovine mycoplasmal infections. Small. Ruminant Res., 2008; 76: 92–98.
DaMassa, A.J, Wakenell, P.S., Brooks, D.L.: Mycoplasmas of goats and sheep. J. Vet. Diagn. Invest., 1992; 4: 101–113. Gonçalves. R., Mariano, I., Nşñez, A., Branco, S., Fairfoul, G., Nicholas, R.: Atypical non-progressive pneumonia in goats. Vet. J., 2010; 183: 219–221.
Rifatbegović, M., Maksimović, Z., Hulaj, B.: Mycoplasma ovipneumoniae associated with severe respiratory disease in goats. Vet. Rec., 2011; 168: 565.
Thiaucourt, F., Bölske, G., Leneguersh, B., Smith, D., Wesonga, H.: Diagnosis and control of contagious caprine pleuropneumoniae. Rev. Sci. Tech., 1996; 15: 1415–1429. Razin, S.: Identification of mycoplasma colonies. In: Razin, S., Tully, J.G., Eds. Methods in Mycoplasmology, Vol. I: Mycoplasma Characterization. Academic Press, Inc., New York. 1983: 83–89.
Poveda, J.B.: Biochemical characteristics in mycoplasma identification. In: Miles, R.J., Nicholas, R.A.J., Eds. Methods in Molecular Biology, Vol. 104: Mycoplasma Protocols. Humana Press, Totowa, NJ, USA. 1998: 69–78.
Poveda, J.B., Nicholas, R.: Serological identification of mycoplasmas by growth and metabolic inhibition tests. In: Miles, R.J., Nicholas, R.A.J., Eds. Methods in Molecular Biology, Vol. 104: Mycoplasma Protocols. Humana Press, Totowa, NJ, USA. 1998: 105–111. van Kuppeveld, F.J, Johansson, K.E., Galama, J.M., Kissing, J., Bölske, G., van der Logt, J.T., Melchers, W.J.: Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR. Appl. Environ. Microb., 1994; 60: 149– 1