Özgecan Korkmaz AĞAOĞLU, Mustafa SAATCI, Özkan ELMAZ, Mehmet ÇOLAK, Mesih KOCAMÜFTÜOĞLU, Emel ZEYTÜNLÜ

Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey

Alpha-lactalbumin plays a key role in lactose synthesis in the mammary glands of domestic animals. This study evaluated Hair (188), Saanen (82), Kilis (63), and Honamli (183) goat breeds, with the primary goal of investigating DNA polymorphism of Mva I RFLP at exon 3 of the alpha-lactalbumin gene. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotype a total of 516 goats for Mva I polymorphism of alpha-lactalbumin. In the breeds that were studied, digestion of the amplification product with the Mva I restriction enzyme revealed 2 alleles (A1 and A2) and 3 genotypes (A1A1, A1A2, and A2A2). Allelic frequencies for Hair, Honamli, Saanen, and Kilis breeds were found to be 0.87, 0.92, 0.87, and 0.85, respectively, for the A1 allele and 0.13, 0.08, 0.13, and 0.15, respectively, for the A2 allele. None of the studied breeds exhibited significant deviation from Hardy–Weinberg equilibrium. Consequently, this study shed light on the polymorphism of alpha-lactalbumin in 4 goat breeds. Another finding of this study was the presence of genetic polymorphism at the alpha-lactalbumin gene in Kilis and Honamli goat breeds, a fact that was not known previously.

Anahtar Kelimeler:

Key words: Alpha-lactalbumin, goat, LALBA, PCR-RFLP, polymorphism

Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey

Keywords:

Key words: Alpha-lactalbumin, goat, LALBA, PCR-RFLP, polymorphism,

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Jenness R. Inter-species comparison of milk proteins. In: Fox, PF, editor. Developments in Dairy Chemistry Proteins, Vol. New York, NY, USA: Applied Science Publishers; 1982. pp. 87–114.
Ebner K, Brodbeck U. Biological role of alpha-lactalbumin: a review. J Dairy Sci 1968; 51: 317–322.
Jain A, Gour DS, Bisen PS, Prashant P, Dubey P, Sharma DK, Joshi BK, Kumar D. Single nucleotide polymorphism (SNP) in alpha-lactalbumin gene of Indian Jamunapari breed of Capra hircus. Small Rum Res 2009; 82: 156–160.
Ma RN, Deng CJ, Zhang XM, Yue XP, Lan XY, Chen H, Lei CZ. S-novel SNP of alpha-lactalbumin gene in Chinese dairy goats. Mol Biol (Mosk) 2010; 44: 608–612.
Bleck GT, Bremel RD. Sequence and single-base polymorphisms of the bovine a-lactalbumin 5’ flanking region. Gene 1993; 126: 213–218.
Gordon WG, Aschaffenburg R, Sen A, Ghosh SK. Amino acid composition of several alpha-lactalbumins. J Dairy Sci 1968; 51: 947–947.
Brew K, Castellino FJ, Vanaman TC, Hill RL. The complete amino acid sequence of bovine α-lactalbumin. J Biol Chem 1970; 245: 4570–4582.
Vilotte JL, Soulier S, Printz C, Mercier JC. Sequence of the goat alpha-lactalbumin-encoding gene: comparison with the bovine gene and evidence of related sequences in the goat genome. Gene 1991; 98: 271–276.
McKenzie HA, White FHJ. Lysozyme and alpha-lactalbumin: structure, function, and interrelationships. Adv Protein Chem 1991; 41: 173–315.
Pike ACW, Brew K, Acharya KR. Crystal structures of guineapig, goat and bovine a-lactalbumin highlight the enhanced conformational flexibility of regions that are significant for its action in lactose synthase. Structure (Camb) 1996; 4: 691–703.
Nakamura T, Makabe K, Tomoyori K, Maki K, Mukaiyama A, Kuwajima K. Different folding pathways taken by highly homologous proteins, goat a-lactalbumin and canine milk lysozyme. J Mol Biol 2010; 396: 1361–1378.
Tomoyori K, Nakamura T, Makabe K, Maki K, Saeki K, Kuwajima K. Sequential four-state folding/unfolding of goat α-lactalbumin and its N-terminal variants. Proteins 2012; 80: 2191–2206.
Hayes H, Popescu P, Dutrilaux B. Comparative gene mapping of lactoperoxidase, retinoblastoma, and alpha-lactalbumin genes in cattle, sheep and goat. Mamm Genome 1993; 4: 593– 5
Qasba PK, Safaya SK. Similarity of the nucleotide sequences of the rat x-lactalbumin and chicken lysozyme genes. Nature 1984; 308: 377–380.
Hall L, Emery DC, Davies MS, Parker D, Craig RK. Organization and sequence of the human alpha-lactalbumin gene. Biochem J 1987; 242: 735–742.
Vilotte JL, Soulier S, Mercier JC, Gaye P, Hue-Delahaie D, Furet JP. Complete nucleotide sequence of bovine alpha lactalbumin gene: comparison with its rat counterpart. Biochimie 1987; 69: 609–620.
Laird JE, Jack L, Hall L, Boulton AP, Parker D, Craig RK. Structure and expression of the guinea-pig alpha-lactalbumin gene. Biochem J 1988; 254: 85–94.
Jung A, Sippel AE, Grez M, Schtitz G. Exons encode functional and structural units of chicken lysozyme. Proc Natl Acad Sci U S A 1980; 71: 5159–5763.
Peters CWB, Kruse U, Pollwein R, Grzeschik KH, Sippel AE. The human lysozyme gene: sequence organization and chromosomal location. Eur J Biochem 1989; 182: 507–516.
Blumberg BS, Tombs MP. Possible polymorphism of bovine α-lactalbumin. Nature 1958; 181: 683–684.
Bell K, Hopper KE, McKenzie HA. Bovine α-lactalbumin C and αS1, β and κ-casein of Bali (Banteng) cattle Bos (Bibos) javanicus. Aust J Biol Sci 1981; 34: 149–159.
Cosenza G, Gallo D, Illario R, Di Gregorio P, Senese C, Ferrara L, Ramunno L. A Mva l PCR-RFLP detecting a silent allele at the goat α-lactalbumin locus. J Dairy Res 2003; 70: 355–357.
Lan XY, Pan CY, Chen H, Zhang CL, Zhang AL, Zhang L, Li JY, Lei CZ. An MspI PCR-RFLP detecting a single nucleotide polymorphism alpha-lactalbumin gene in goat. Czech J Anim Sci 2007; 52: 138–142.
Lan XY, Chen H, Tian ZQ, Liu SQ, Zhang YB, Wang X, Fang XT. Correlations between SNP of LALBA gene and economic traits in Inner Mongolian white cashmere goat. Hereditas (Beijing) 2008; 30: 169–174.
TAGEM. Türkiye Evcil Hayvan Genetik Kaynakları Tanıtım Kataloğu. Ankara, Turkey: TAGEM; 2009 (in Turkish).
Porter V. Goats of the World. Ipswich, UK: Farming Press; 19 Yalçın BC. Sheep and Goats in Turkey. Rome, Italy: Food and Agriculture Organization of the United Nations; 1986.
Korkmaz Ağaoğlu Ö, Ertuğrul O. Assessment of genetic diversity, genetic relationship and bottleneck using microsatellites in some native Turkish goat breeds. Small Rum Res 2012; 105: 53–60.
Öner Y, Şahin Ş, Elmacı C. Gökçeada, Saanen ve Kıl Keçisi ırklarının LALBA/Mva I polimorfizmi bakımından incelenmesi. Hayvansal Üretim 2012; 53: 55–59 (in Turkish).