Investigation of the possibilities of using PON1 enzyme activity in animal improvement
Investigation of the possibilities of using PON1 enzyme activity in animal improvement
In this study, the activity of paraoxonase 1 (PON1) in various cattle races in different age groups was investigated. PON1 iscapable of hydrolyzing organophosphate agents of pesticides that are amongst the most significant stress sources for farm animals. PON1can also hydrolyse nerve gases and can protect the organism against lipid peroxide formations that occur as a result of oxidation of LDL.The cattle races in the study were separated into 3different age groups of young (0 to6 months), middle-age (6 months to 4 years) and old(4 years of age and above), and 5 mL of blood sample was collected from each animal to analyse and determine their PON1 activities.The statistical analyses were then performed. Based on the knowledge that the animals with high PON1 enzyme activity would havehigher adaptation capabilities for regions that are being sprayed with pesticides compared to animals with low PON1 enzyme activity,the possibility of using PON1 activity tests as a potential precharacterization study for marker assisted selection (MAS) was investigated.The results have revealed that PON1 enzyme activity could be used as a biomarker for determination of pesticide resistance.
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- 1. Akçapınar H, Özbeyaz C. Hayvan Yetiştiriciliği Temel Bilgileri.
Ankara, Turkey: Nadir Kitap; 1999 (in Turkish).
- 2. Aksoy AR. Hayvan Islahı Ders Notları. Kars, Turkey: Kafkas
Üniversitesi Veteriner Fakültesi; 2003 (in Turkish).
- 3. Montaldo HH, Meza-Herrera CA. Use of molecular markers
and major genes in the genetic improvement of livestock
Electron. Journal of Biotechnology 1998; 1: 83-89.
- 4. Lara M, Gama L, Bufarah G, Sereno J, Celegato E et al. Genetic
polymorphisms at the k-casein in the pantaneiro cattle.
Archivos de Zootecnia 2002; 51: 99-105.
- 5. Bal SH, Budak F. Genomik proteomik kavramlarına genel bakış
ve uygulama alanları. Uludağ Üniversitesi Tıp Fakültesi Dergisi
2013; 39: 65-69 (in Turkish with an abstract in English).
- 6. Tiryaki O, Temur C. The fate of pesticide in the environment.
Journal of Biological and Enviromental Sciences 2010; 4: 29-
38.
- 7. Tarakçı Ü, Türel İ. Halk sağlığı amaçlı kullanılan pestisitlerin
(Biyosidal) güvenilirlik standartlarının karşılaştırılması. YYU
Veteriner Fakültesi Dergisi 2009; 20: 11-18 (in Turkish with an
abstract in English).
- 8. Nougadere A, Merlo M, Heraud F, Rety J, Truchot E et al. How
dietary risk assessment can guide risk management and food
monitoring programmes: The approach and results of the
French Observatory on Pesticide Residues (ANSES/ORP).
Food Control 2014; 41: 32-48.
- 9. Costa L, Li W, Richter R, Shih D, Lusis A et al. The role of
paraoxonase (PON1) in the detoxication of organophosphates
and its human polmorphisms. Chemico Biological Interactions
1999; 429: 119-120.
- 10. Graham A, Hassall DG, Rafique S, Owen JS. Evidence for a
paraoxonase-independent inhibition of low density lipoprotein
oxidation by high-density lipoprotein. Atherosclerosis 1997;
135: 193-204.
- 11. Aviram M, Hardak E, Vaya J, Mahmood S. Human serum
paraoxonase (PON1) Q and R electively decrease lipid
peroxides in human coronary and carotid lesions. Circulation
1999; 101: 2510-17.
- 12. Antonic-Svetina M, Turk R, Svetina A, Geres D, Rekic B et al.
Lipid statusparaoxonase-1 activity and metabolic parametres
in serum of heifers and lactatinh cows related to oxidative
stress. Research in Veterinary Science 2011; 90: 298-300.
- 13. Giordino A, Veronesi M, Rossi G, Pezzia F, Probo M et al.
Serum paraoxonase-1 activity in neonatal calves: Age related
variations and comparison between healthy and sick animals.
Veterinary Journal 2013; 197: 499-501.
- 14. Erzengin M, Demir D, Arslan M, Sinan S. Purification and
characterization of paraoxonase 1 (PON1) from Swiss Black
Holstein and Montofon bovines. Applied Biochemistry
Biotechnology 2014; 173: 1597-1660.
- 15. Richter R, Clement E. Determination of paraoxonase (PON1)
status requires more than genotyping. Pharmacogenetics 1999;
9: 745-53.
- 16. Richter RJ, Jampsa RL, Jarvik GP, Costa LG, Furlong CE.
Determination of paraoxonase 1 status and genotypes at
specific polymorphic sites. Current Protocols in Toxicology
2004. doi: 10.1002/0471140856.tx0412s19
- 17. Eckerson HW, Romson J, Wyte C, La Du B. The human serum
paraoxonase polymorphism: identification of phenotypes by
their response to salts. American Journal of Human Genetics
1983; 35(2): 214-227.
- 18. Davies HG, Richter RJ, Keifer M, Broomfield CA, Sowalla J et
al. The effect of the human serum paraxonase polmorphism
is reversed with diazoxon soman and sarin. Nature Genetics
1996; 14: 334-336.
- 19. Costa L, Cole T, Vitalone A, Furlong C. Measurement of
paraoxonase (PON1) status as a potential biomarker of
susceptibility to organophosphate toxicity. Clinica Chimica
Acta 2005; 352: 37-47.
- 20. Hillel J, Dunninton E, Siegel P. DNA markers in poultry
breeding and genetic analyses. Poultry Science 1992; 4: 169-
186.
- 21. Nicholas F. Introduction to Veterinary Genetics. Oxford, UK:
Oxford University Press; 1996.
- 22. Kulka M, Beltowski J, Klucinski W, Orlowska M, Kolodziejska
J et al. Serum paraoxonase-1 activity of dairy Holstein-Fresian
cows in different lactation stages–preliminary study. Polish
Journal of Veterinary Sciences 2014; 17: 143-147.
- 23. Kulka M, Kolodziejska-Lesisz J, Klucinski W. Serum
paraoxonase (PON1) activity and lipid metabolism parametres
changes in different production cycle periods of holsteinfriesion
an Polish red and Norwegian breeds. Polish Journal of
Veterinary Sciences 2016; 19: 165-73.
- 24. Turk R, Juretic D, Geres D, Svetina A, Turk N et al. Influence
of oxidative stress and metabolic adaptation on PON1 activity
and MDA level in transition dairy cows. Animal Reproduction
Science 2008; 108(1-2): 98-106.
- 25. Dileep K, Syed İR. Age-dependent paraoxonase 1 (PON1)
activity and LDL oxidation in Wistar rats during their entire
lifespan. Scientific World Journal 2014; 1-6.
- 26. Taha N, El-Noeman S, Korshom M, Mandour A, El-Feky M.
(2010) Serum glutathione peroxidase and paraoxonase-1
enzyme activities in camel (Camelus dromedarius): effect of
age and sex differences. Journal of Camel Practice and Research
2010; 17: 1-8.
- 27. Wan-Fen L, Lucio G, Clement E. Serum paraoxonase status: a
major factor in determining resistance to organophosphates.
Journal of Toxicology Environmental Health 1993; 40: 337-
346.
- 28. Stoltz DA, Ozer EA, Taft PJ, Barry M, Liu L et al. Drosophila
are protected from Pseudomonas aeruginosa lethality by
transgenic expression of paraoxonase-1. Journal of Clinical
Investigation 2008; 118: 3123-3131.