Claudıne C. TERCIÑO, Carl Leonard M. PRADERA, Melvın A. BAGOT

Assessment of the molluscicidal activity of wormwood Artemisia dubia, Wallich leaves ethanolic extract on Oncomelania hupensis quadrasi, Möllendorff

This study assessed the in vitro molluscicidal activity of Artemisia dubia wormwood leaf ethanolic extract against adult and juvenile Oncomelania hupensis quadrasi and profiled its qualitative phytochemical content. The plants and snails were collected locally. There were 8 concentrations used for adult and juvenile snails: distilled water and 1% ethanol as negative controls; niclosamide 2 mg/L as a positive control; and different concentrations of wormwood ethanolic extracts for adult snails including 3.98% T1 , 4.46% T2 , 5% T3 , 5.61% T4 , and 6.30% T5 and for juvenile snails including 7.94% T1 , 8.91% T2 , 10% T3 , 11.22% T4 , and 12.59% T5 . Each treatment was replicated 5 times with 20 snails per replicate. Results showed that the concentrations that were statistically comparable with niclosamide 2 mg/L were 6.30% T5 with 92% mortality for adults and 10% T3 , 11.22% T4 , and 12.59% T5 for juveniles with 94%, 95%, and 98% mortality, respectively P > 0.05 . The qualitative phytochemical tests for secondary metabolites revealed the presence of tannins, saponins, and terpenoids. Independently or in combination, these secondary metabolites may be responsible for the mortality of the snails. This study indicates the possibility of using wormwood ethanolic extract as a potent and a possible alternative for synthetic molluscicides.

Keywords:

In vitro mortality, schistosomiasis, snails,

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1. Han B, Chen J, Yang X, Wang S, Li C et al. Molluscicidal activities of medicinal plants from eastern China against Oncomelania hupensis, the intermediate host of Schistosoma japonicum. Revista Brasileira de Farmacognosia 2010; 20 (5): 712-718. doi.org/10.1590/S0102-695X2010005000018
2. Kloos H, McCullough FS. Plant molluscicides. Planta Medica 1982; 46 (4): 195-209. doi.org/10.1055/s-2007-971215
3. Sun F, Zhang JF, Wen LY. Research progress on the molluscicidal effect of niclosamide compounded with other molluscicides against Oncomelania hupensis. Chinese Journal of Parasitology & Parasitic Diseases 2014; 32 (1): 72-75.
4. Joshi RC, Meepagala KM, Sturtz G, Cagauan AG, Mendoza CO et al. Molluscicidal activity of vulgarone B from Artemisia douglasiana (Besser) against the invasive, alien, mollusc pest, Pomacea canaliculata (Lamarck). International Journal of Pest Management 2005; 51 (3): 175-180.
5. Prabhakaran G, Bhore S, Ravichandran M. Development and evaluation of poly herbal molluscicidal extracts for control of apple snail (Pomacea maculata). Agriculture 2017; 7 (3): 22.
6. Zeb S, Ali A, Zaman W, Zeb S, Ali S et al. Pharmacology, taxonomy and phytochemistry of the genus Artemisia specifically from Pakistan: a comprehensive review. Pharmaceutical and Biomedical Research 2018; 4 (4): 1-12. doi: 10.18502/pbr.v4i4.543
7. Abid R, Qaiser M. Cypsela morphology and its taxonomic significance of the genus Artemisia L. (AnthemideaeAsteraceae) from Pakistan. Pakistan Journal of Botany 2008; 40 (5): 1827-1837.
8. Fernandez TJ Jr, Landerito EO, Acabal AM. Development of the herbal drugs for the management of common stronglye worm infection in goats. In: Los Baños Laguna; 2009.
9. Alinsub CJ, Bagot M. In vitro molluscicidal activity of wormwood (Artemisia dubia) leaves against Oncomelania hupensis quadrasi. Annals of Tropical Research 2019; 41: 16- 23. doi: 10.32945/atr4112.2019
10. Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine 2014; 4: S359-S367. doi: 10.12980/APJTB.4.2014C1030
11. Ponder WF, Hallan A, Shea M, Clark SA. Australian Freshwater Mollusks. Canberra, Australia: The Australian Museum; 2016.
12. Chua JC, Tabios IB, Tamayo PP, Leonardo LR, Fontanilla IC et al. Genetic comparison of Oncomelania hupensis quadrasi (Möllendorf, 1895) (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma japonicum in the Philippines, based on 16S ribosomal RNA sequence. Science Diliman 2017; 29: 2.
13. Kusin AA, Bagot MA, Lumain, JPLM. In vitro acaricidal efficacy of neem (Azadirachta indica) oil against ear mites (Otodectes cynotis). Journal of Agriculture and Technology Management 2016; 19: 15-19.
14. Meepagala KM, Sturtz G, Mischke, CC, Wise D, Duke SO. Molluscicidal activity of vulgarone B against ram’s horn snail (Planorbella trivolvis). Pest Management Science 2004; 60 (5): 479-482.
15. Sharma M, Devkota A. Allelopathic influences of Artemisia Dubia Wall. ex. Besser on seed germination and seedling vigor of Parthenium hysterophorus L. Journal of Institute of Science and Technology 2018; 22 (2): 117-128.
16. Sivagnanam SK, Rao RK, Mudiganti DU, Jeelani PG. Preliminary phytochemical analysis of Artemisia amygdalina, Nerium odorum and Strychnos potatorum. Journal of Pharmacy Research 2012; 5 (7): 3734-3739.
17. Hussein Ayoub SM, Yankov LK. The molluscicidal factor of tannin-bearing plants. International Journal of Crude Drug Research 1986; 24 (1): 16-18. doi: 10.3109/13880208609060881
18. Mølgaard P. Food plant preferences by slugs and snails: a simple method to evaluate the relative palatability of the food plants. Biochemical Systematics and Ecology 1986; 14 (1): 113- 121. doi: 10.1016/0305-1978(86)90095-5
19. Schaufelberger D, Hostettmann K. On the molluscicidal activity of tannin containing plants. Planta Medica 1983; 48 (6): 105-107. doi: 10.1055/s-2007-969899
20. Bezerra JCB, Silva IA, Ferreira HD, Ferri PH, Santos SC. Molluscicidal activity against Biomphalaria glabrata of Brazilian Cerrado medicinal plants. Fitoterapia 2002; 73 (5): 428-430. doi: 10.1016/S0367-326X(02)00121-1
21. Musman M, Karina S, Rizki F. Saponins extract from Barringtonia racemosa as molluscicide to brackishwater pond snails (Cerithidea cingulata). International Journal of Applied Research and Technology 2014; 3 (6): 92-97.
22. Suter R, Tanner M, Borel C, Hostettmann K, Freyvogel TA. Laboratory and field trials at Ifakara (Kilombero District, Tanzania) on the plant molluscicide Swartzia madagascariensis. Acta Tropica 1986; 43 (1): 69-83.
23. Hostettmann K, Kizu H, Tomimori T. Molluscicidal properties of various saponins. Planta Medica 1982; 44 (1): 34-35.
24. Moses T, Papadopoulou KK, Osbourn A. Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives. Critical Reviews in Biochemistry and Molecular Biology 2014; 49 (6): 439-462. doi: 10.3109/10409238.2014.953628
25. Hammami H, Mezghani-Jarraya R, Damak M, Ayadi A. Molluscicidal activity of various solvent extracts from Solanum nigrum var. villosum L. aerial parts against Galba truncatula. Parasite 2011; 18 (1): 63-70. doi: 10.1051/parasite/2011181063
26. Hmamouchi M, Lahlou M, Agoumi A. Molluscicidal activity of some Moroccan medicinal plants. Fitoterapia 2000; 71 (3): 308-314. doi.: 10.1016/S0367-326X(99)00152-5
27. Srivastava AK, Singh VK. Biological action of essential oils (terpenes). International Journal of Biological and Medical Research 2019; 10 (3): 6854-6859.
28. Knobloch K, Pauli A, Iberl B, Weigand H, Weis N. Antibacterial and antifungal properties of essential oil components. Journal of Essential Oil Research 1989; 1 (3): 119-128. doi: 10.1080/10412905.1989.9697767
29. McGarvey DJ, Croteau R. Terpenoid metabolism. Plant Cell 1995; 7 (7): 1015.
30. Lemma A. Laboratory and field evaluation of the molluscicidal properties of Phytolacca dodecandra. Bulletin of the World Health Organization 1970; 42 (4): 597.
31. Mohammed A. Why are early life stages of aquatic organisms more sensitive to toxicants than adults? In: Gowder S (editor). New Insights into Toxicity and Drug Testing. Rijeka, Croatia: InTech; 2013. pp. 49-62. doi.: 10.5772/55187
32. Rand GM. Fundamentals of Aquatic Toxicology: Effects, Environmental Fate and Risk Assessment. Boca Raton, FL, USA: CRC Press; 1995.
33. Pavarini DP, Pavarini SP, Niehues M, Lopes NP. Exogenous influences on plant secondary metabolite levels. Animal Feed Science and Technology 2012; 176 (1-4): 5-16.
34. Sampaio BL, Edrada-Ebel R, Da Costa FB. Effect of the environment on the secondary metabolic profile of Tithonia diversifolia: a model for environmental metabolomics of plants. Scientific Reports 2016; 2016: 6. doi: 10.1038/srep29265