Intrauterine insemination with cat semen frozen with various extenders

The aim of this study was to investigate the post-thaw motility and morphological defect rates of cat semen frozen with various extenders, and pregnancy rates after intrauterine insemination (IUI). Cats, 2 males and 14 queens, 2 to 3 years of age, and under the same managemental conditions were used. Semen was collected by means of an electro-ejaculator under general anesthesia. Based on spermatological traits, semen samples presumed suitable for freezing were extended with either tris-fructose-citric acid (EY-TFC) containing 20% egg yolk, or skimmed milk-glucose-taurine (EY-SMGT) containing 10% egg yolk extender, and frozen in 0.25 mL straws. For the extenders, post-thaw motility and morphological abnormality values were found to be efficient for IUI and no statistical significance was observed between the values of the extender groups. To achieve ovulation, queens showing behavioral estrus received 250 IU of hCG via IM route, 30 h prior to IUI. Under general anesthesia, queens were laparatomised on the medial line and the left and right uterine horn were taken out. Into both uterine horns, 2 straws containing 25 × 106 sperm each were thawed at 37 °C for 30 s and equally injected. Pregnancy was diagnosed using an ultrasonography device on the 19th day after IUI by detecting the embryonic vesicles. Of the 3 EY-TFC group queens found to be pregnant, 1 aborted and other 2 had early embryonic death (EED); the pregnancy rate was 42.90%. In a queen in the EY-SMGT group, no ovulation took place after the hCG injection. In 2 of the queens found to be pregnant in this group, EED occurred and the pregnancy rate was 33.30%. In conclusion, cat semen extended and frozen with EY-TFC and EY-SMGT extenders was found to be successful in post-thaw motility and morphological defect rates. Therefore, surgical IUI may be more effective for feline AI using frozen semen rather than intravaginal insemination.

Intrauterine insemination with cat semen frozen with various extenders

The aim of this study was to investigate the post-thaw motility and morphological defect rates of cat semen frozen with various extenders, and pregnancy rates after intrauterine insemination (IUI). Cats, 2 males and 14 queens, 2 to 3 years of age, and under the same managemental conditions were used. Semen was collected by means of an electro-ejaculator under general anesthesia. Based on spermatological traits, semen samples presumed suitable for freezing were extended with either tris-fructose-citric acid (EY-TFC) containing 20% egg yolk, or skimmed milk-glucose-taurine (EY-SMGT) containing 10% egg yolk extender, and frozen in 0.25 mL straws. For the extenders, post-thaw motility and morphological abnormality values were found to be efficient for IUI and no statistical significance was observed between the values of the extender groups. To achieve ovulation, queens showing behavioral estrus received 250 IU of hCG via IM route, 30 h prior to IUI. Under general anesthesia, queens were laparatomised on the medial line and the left and right uterine horn were taken out. Into both uterine horns, 2 straws containing 25 × 106 sperm each were thawed at 37 °C for 30 s and equally injected. Pregnancy was diagnosed using an ultrasonography device on the 19th day after IUI by detecting the embryonic vesicles. Of the 3 EY-TFC group queens found to be pregnant, 1 aborted and other 2 had early embryonic death (EED); the pregnancy rate was 42.90%. In a queen in the EY-SMGT group, no ovulation took place after the hCG injection. In 2 of the queens found to be pregnant in this group, EED occurred and the pregnancy rate was 33.30%. In conclusion, cat semen extended and frozen with EY-TFC and EY-SMGT extenders was found to be successful in post-thaw motility and morphological defect rates. Therefore, surgical IUI may be more effective for feline AI using frozen semen rather than intravaginal insemination.

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Bibtex @ { tbtkveterinary150770, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2011}, volume = {35}, pages = {311 - 318}, doi = {}, title = {Intrauterine insemination with cat semen frozen with various extenders}, key = {cite}, author = {Baran, Alper and Tek, Çağatay and Demir, Kamber and Sabuncu, Ahmet and Özdaş, Özen Banu} }
APA Baran, A , Tek, Ç , Demir, K , Sabuncu, A , Özdaş, Ö . (2011). Intrauterine insemination with cat semen frozen with various extenders . Turkish Journal of Veterinary and Animal Sciences , 35 (5) , 311-318 .
MLA Baran, A , Tek, Ç , Demir, K , Sabuncu, A , Özdaş, Ö . "Intrauterine insemination with cat semen frozen with various extenders" . Turkish Journal of Veterinary and Animal Sciences 35 (2011 ): 311-318 <
Chicago Baran, A , Tek, Ç , Demir, K , Sabuncu, A , Özdaş, Ö . "Intrauterine insemination with cat semen frozen with various extenders". Turkish Journal of Veterinary and Animal Sciences 35 (2011 ): 311-318
RIS TY - JOUR T1 - Intrauterine insemination with cat semen frozen with various extenders AU - Alper Baran , Çağatay Tek , Kamber Demir , Ahmet Sabuncu , Özen Banu Özdaş Y1 - 2011 PY - 2011 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 311 EP - 318 VL - 35 IS - 5 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Intrauterine insemination with cat semen frozen with various extenders %A Alper Baran , Çağatay Tek , Kamber Demir , Ahmet Sabuncu , Özen Banu Özdaş %T Intrauterine insemination with cat semen frozen with various extenders %D 2011 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 35 %N 5 %R %U
ISNAD Baran, Alper , Tek, Çağatay , Demir, Kamber , Sabuncu, Ahmet , Özdaş, Özen Banu . "Intrauterine insemination with cat semen frozen with various extenders". Turkish Journal of Veterinary and Animal Sciences 35 / 5 (Mayıs 2011): 311-318 .
AMA Baran A , Tek Ç , Demir K , Sabuncu A , Özdaş Ö . Intrauterine insemination with cat semen frozen with various extenders. Turkish Journal of Veterinary and Animal Sciences. 2011; 35(5): 311-318.
Vancouver Baran A , Tek Ç , Demir K , Sabuncu A , Özdaş Ö . Intrauterine insemination with cat semen frozen with various extenders. Turkish Journal of Veterinary and Animal Sciences. 2011; 35(5): 311-318.
IEEE A. Baran , Ç. Tek , K. Demir , A. Sabuncu ve Ö. Özdaş , "Intrauterine insemination with cat semen frozen with various extenders", Turkish Journal of Veterinary and Animal Sciences, c. 35, sayı. 5, ss. 311-318, May. 2011
Turkish Journal of Veterinary and Animal Sciences
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

61.1b55.8b

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