Atila ATEŞ, Melih UÇMAK, Feraye Esen GÜRSEL, Gamze EVKURAN, Özge Turna YILMAZ, Mehmet C. GÜNDÜZ, Merih Duygu TÖRE, Sinem Ö. ENGİNLER, Güven KAŞIKÇI

Can the vascular endothelial growth factor (VEGF) and total estradiol 17β be used as a marker of equine oocyte maturation?

Bu çalışmanın amacı folliküler sıvı (FS) ve serumda vasküler endotelyal büyüme faktörü (VEBF) ve total östradiol 17β’yı belirlemek ve bu maddelerin seviyelerini oosit maturasyonu ile ilişkilendirmektir. Follikül çapı 35 mm’den büyük olduğunda; oosit toplama amacıyla transvajinal ultrason rehberliğinde follikül aspirasyonu gerçekleştirildi. Kısraklar yaşa göre iki gruba ayrıldı ve VEBF ve östradiol 17β değişimleri folliküler sıvı ve serumda incelendi. Folliküler sıvı ve serum östradiol 17β seviyeleri genç (4-10 yaş) ve yaşlı (20-24 yaş) kısrakların arasında farklı bulunmadı (P > 0,05). Yaşlı kısrakların folliküler sıvı ve serum VEBF konsantrasyonları genç kısraklardakinden daha düşük bulundu (P < 0,001). Genç kısraklardan elde edilen VEFB seviyesi mature ve intermediate oositler arasında fark göstermezken; yaşlı kısraklardan elde edilen VEFB seviyesi mature oositlerde intermediate ve immature oositlere göre daha yüksek bulundu (P < 0,01). Yapılan çalışma sonucunda; VEBF’nün folliküler büyüme ve gelişimde önemli bir rol oynayabileceği, VEBF seviyelerinin yaşa bağımlı gözüktüğü ve bu nedenle VEFB seviyeleri oosit maturasyonun değerli biyokimyasal belirleyicisi olabileceği kanısına varıldı.

Vasküler endotelyal büyüme faktörü (VEBF) ve total östradiol 17β kısrak oosit maturasyonunda bir belirleyici olarak kullanılabilir mi?

The aim of this study was to quantitate vascular endothelial growth factor (VEGF) and estradiol-17β in follicular fluid (FF) and serum and to correlate the levels of these substances with oocyte maturation. Transvaginal ultrasound-guided follicular aspirations were performed with the purpose of oocyte collection when the follicle was >35 mm in diameter. The mares were divided into 2 groups according to age, and the changes in VEGF and estradiol- 17β in follicular fl uid and serum were examined. Levels of estradiol-17β in the follicular fl uid and serum of young mares (4-10 years old) did not differ from those in old mares (20-24 years old) (P > 0.05). The follicular fl uid and serum concentrations of VEGF in old mares were lower (P < 0.001) than those in young mares. The VEGF levels in young mares did not differ in cases when either mature or intermediate oocytes were collected, whereas its levels in old mares were higher (P < 0.01) when mature oocytes were collected than when intermediate and immature oocytes were collected. Our data suggest that VEGF may play an important role in follicular growth and development, that VEGF levels appear to be age-dependent, and that VEGF levels might be valuable biochemical markers of oocyte maturation.

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1. Tonetta, S.A., Dizerega, G.S.: Intragonadal regulation of follicular maturation. Endocr. Rev., 1989; 10: 205-229.
2. Redmer, D.A., Reynolds, L.P.: Angiogenesis in the ovary. Rev. Reprod., 1996; 1: 182-192.
3. Ferrara, N., Davis-Smyth, T.: The biology of vascular endothelial growth factor. Endocr. Rev., 1997; 18: 4-25.
4. Hazzard, T.M., Christenson, L.K., Stouffer, R.L.: Changes in expression of vascular endothelial growth factor (VEGF), angiopoietin (Ang)-1 and Ang-2 in the macaque corpus luteum during the menstrual cycle. Mol. Hum. Reprod., 2000; 6: 993-998.
5. Christenson, L.K., Stouffer, R.L.: Follicle-stimulating hormone and luteinizing hormone/chorionic gonadotropin stimulation of vascular endothelial growth factor production by macaque granulosa cells from pre- and periovulatory follicles. J. Clin. Endocrinol. Metab., 1997; 82: 2135-2142.
6. Ravindranath, L., Little-Ihring, L.L., Phillips, H.S., Ferrara, N., Zeleznil A.S.: Vascular endothelial growth factor messenger ribonucleic acid expression in primate ovary. Endocrinology, 1992; 131: 254-260.
7. Kamat, B.R., Brown, L.F., Manseau, E.J., Senger, D.R., Dvorak, H.F.: Expression of VPF/VEGF by human granulosa and theca lutean cells: role in corpus luteum development. Am. J. Pathol., 1995; 146: 157-165.
8. Lee, A., Christenson, L.K., Stouffer, R.L., Burry, K.A., Patton, P.E.: Vascular endothelial growth factor levels in serum and follicular fluid of patients undergoing in vitro fertilization. Fertil. Steril., 1997; 68: 305-311.
9. Anasti, J.N., Kalantaridou, S.N., Kimzey, L.M., George, M., Nelson N.M.: Human follicle fluid vascular endothelial growth factor concentrations are correlated with luteinization in spontaneously developing follicles. Hum. Reprod., 1998; 13: 1144-1147.
10. Moncayo, H.E., Penz-Koza, A., Marth, C., Gasti, G., Herold, M., Moncaya, R.: Vascular endothelial growth factor in serum and in the follicular fluid of patients undergoing hormonal stimulation for in-vitro fertilization. Hum. Reprod., 1998; 13: 3310-3314.
11. Friedman, C.I., Arbogast, L., Danforth, D.R.: Follicular fl uid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction. Fertil. Steril., 1997; 68: 607-612.
12. Manau, D., Balasch, J., Jimenez, W., Fabregues, F., Civico, S., Casamitjana, R., Creus, M., Vanrell, JA.: Follicular fl uid concentrations of adrenomedullin, vascular endothelial growth factor and nitric oxide in IVF cycles: relationship to ovarian response. Hum. Reprod., 2000; 15: 1295-1299.
13. Friedman, C.I., Seifer, D.B., Kennard, E.A., Arbogast, L., Alak, B., Danforth, D.R.: Elevated level of follicular fl uid vascular endothelial growth factor is a marker of diminished pregnancy potential. Fertil. Steril., 1998; 70: 836-839.
14. Fishel, S.B., Edwards, R.G., Walters, D.E.: Follicular steroids as prognosticator of successful fertilization of human oocytes in vitro. J. Endocrinol., 1983; 99: 335-344.
15. Kreiner, D., Liu, H.C., Itskovitz, J., Veeck, L., Rosenwaks, Z.: Follicular fluid oestradiol and progesterone are markers of preovulatory oocyte quality. Fertil. Steril., 1987; 48: 991-997.
16. Enien, W.M., El Sahwy, S., Harris, C.P., Seif, M.W., Elstein, M.: Human chorionic gonadotrophin and steroid concentrations in follicular fluid: the relationship to oocyte maturity and fertilization rates in stimulated and natural in vitro fertilization cycles. Hum. Reprod., 1995; 10: 2840-2844.
17. Messinis, I.E., Templeton, A.A.: Relationship between intrafollicular levels of prolactin and sex steroids and in vitro fertilization of human oocytes. Hum. Reprod., 1987; 2: 607- 609.
18. Stone, B.A., Vargyas, J.P.M., Mars, R.P., Quinn, P.J., Batzofi n, J.H., Tan, T., Kerin, J.F.P., Serafini, P.C.: Levels of steroids and protein hormones in antral fluids of women treated with different combinations of gonadotrophins, clomiphene citrate and a gonadotrophin-releasing hormone analog. Fertil. Steril., 1988; 49: 249-257.
19. Tavmergen, E., Tavmergen, E.N., Capanoglu, R.: Do analogues of gonadotrophin releasing hormone infkuence follicular fluid steroids, oocyte quality and fertilization rates? Hum. Reprod., 1992; 7: 479-482.
20. Gündüz, M.C., Turna, O., Apaydın, S., Ucmak, M., Kasıkcı G., Ates, A., Esen, F., Evkuran, G., Töre D.: Potential relevance of insulin-like growth factor (IGF-I), total estriol and progesterone to the in vitro maturation of equine oocytes. Anim. Reprod. Sci., 2010; 121S: 245-246.
21. Kawano, Y., Hasan, K.Z., Fukuda, J., Mine, S., Miyakawa, I.: Production of vascular endothelial growth factor and angiogenic factor in human follicular fluid. Molecular and Cellular Endocrinology, 2003; 202: 19-23.
22. Malamitsi-Puchner, A., Sarandakau, A., Baka, S.G., Tziotis, J., Rizos, D., Hassiakos, D., Creatsas, G.: Concentrations of angiogenic factors in follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization: association with oocyte maturity and fertilization. Fertil. Steril., 2000; 76: 98-1011.