Influence of β-1,3/1,6 glucan applications on some non-specific cellular immune response and haematologic parameters of healthy Nile tilapia (Oreochromis niloticus L., 1758)

Bu araştırmada, Nil tilapia (Oreochromis niloticus L.)’larında farklı dozlarda β-glukan uygulamasının özel olmayan immun yanıt ve hematolojik parametreler üzerine etkisi araştırılmıştır. Balıklar iki hafta boyunca % 0,5 ve % 0,1 oranlarında (deney) glukan içeren ve glukan içermeyen (kontrol) yemlerle beslenmişlerdir. İki farklı dozla iki haftalık besleme sonrasında, eritrosit (RBC), lökosit (WBC), hematokrit (Hct), hemoglobin (Hb) miktarı, lökosit hücre tipleri (lenfosit, monosit, nötrofil, eosinofil yüzdeleri), fagositik aktivite miktarı, eritrosit hücre indeksleri (MCV, MCH) ve kan hücre büyüklükleri tayin edilmiştir. Denemenin sonunda, % 0,1 oranında glukan ile beslenen balıklarda WBC ve fagositik aktivite miktarları, lenfosit ve monosit yüzdeleri, % 0,5 oranındaki glukan ve kontrol gruplarından daha yüksek bulunmuştur (P < 0,05). RBC miktarı da deney gruplarında, kontrol grubundan daha yüksek bulunmuştur. Gruplar arasında Hb, Hct, MCV, MCH, nötrofil, eosinofil yüzdeleri ve hücre büyüklüklerinde herhangi bir farklılık gözlenmemiştir (P > 0,05).

β-1,3/1,6 glukan uygulamalarının sağlıklı Nil tilapia (Oreochromis niloticus L., 1758)’larında bazı hematolojik parametreler ve özel olmayan hücresel immun yanıt üzerine etkisi

In this study, the effects of β-glucan administration at different dosages on non-specific immune response and haematological parameters in healthy Nile tilapia (Oreochromis niloticus L.) were investigated. Fish were fed with diets containing 0.5% and 0.1% glucan (experiment) and without glucan (control) for 2 weeks. After feeding for 2 weeks with 2 different dosages, erythrocyte (RBC), leukocyte (WBC), hematocrit (Hct), haemoglobin (Hb) amount, leukocyte cell types (percentage of lymphocyte, monocyte, neutrophil, eosinophil), the values of phagocytic activities, erythrocyte cell indexes (MCV and MCH) and blood cell sizes were determined. WBC and phagocytic activity amounts, percentages of lymphocyte and monocyte of fish fed with the diets containing 0.1% glucan were higher than 0.5% glucan and control groups (P < 0.05). RBC amount was found to be higher in the experimental groups than in the control group. There were no differences between groups in terms of Hb, Hct, MCV, MCH, neutrophil, eosinophil, and cell sizes values (P > 0.05).

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1. Verlhac, V., Obach, A., Gabaudan, J., Schüep, W., Hole, R.: Immunomodulation by dietary vitamin C and glucan in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol., 1998; 8: 409-424.
2. Raa, J.: The use of immunostimulatory substances in fish and shellfish farming. Rev. Fish. Sci., 1996; 4: 229-288.
3. Sakai, M.: Current research status of fish immunostimulants. Aquaculture, 1999; 172: 63-92.
4. Raa, J.: The use of immune-stimulants in fish and shellfish feeds. Fifth International Symposium on Aquaculture Nutrition. 19-22 November 2000. Mérida, Yucatán, Mexico.
5. Jeney, G., Anderson, D.P.: Glucan injection or bath exposure given alone or in combination with a bacterin enhance the nonspecific defence mechanisms in rainbow trout (Oncorhynchus mykiss). Aquaculture, 1993; 116: 315-329.
6. Cook, M.T., Hayball, P.J., Hutchinson, W., Nowak, B., Hayball, J.D.: The efficiency of a commercial β-glucan preparation, EcoActiva, on stimulating respiratory burst activity of headkidney macrophages from pink snapper (Pagrus auratus), Sparidae. Fish Shellfish Immunol., 2001; 11: 661-672.
7. Karataş Düğenci, S., Candan, A.: The effect of some immunostimulants on the non-specific immune system of rainbow trout (Oncorhynchus mykiss, Walbaum). Turk. J. Vet. Anim. Sci., 2003; 27: 1253-1260. (article in Turkish, with an abstract in English)
8. Jeney, G., Galeotti, M., Volpatti, D., Jeney, Z., Anderson, D.P.: Prevention of stress in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture, 1997; 154: 1-15.
9. Blaxhall, P.C., Daisley, K.W.: Routine haematological methods for use with fish blood. J. Fish Biol., 1973; 5: 771-781.
10. Kocabatmaz, M., Ekingen, G.: Standardization of haematological methods and taking blood from various fish species. Doğa Bilim Derg., 1984; 8: 149-159. (article in Turkish, with an abstract in English)
11. Şahan, A., Cengizler, İ.: Determination of some heamatological parameters in spotted barb (Capoeta barroisi, Lortet, 1894) and roach (Rutilus rutilus, Linnaeus, 1758) living in Seyhan River (Adana City Region). Turk. J. Vet. Anim. Sci., 2002; 26: 849-858. (article in Turkish, with an abstract in English)
12. Stolen, S.J., Fletcher, T.C., Rowley, A.F., Zelikoff, J.T., Kaattari, S.L., Smith, S.A.: Techniques in Fish Immunology. Fish Immunology Technical Communications No. 3, 1st edn., SOS Publications. Virginia–Maryland, USA. 1994.
13. Fujimaki, Y., Isoda, M.: Fine-structural study of leukocytes in the goldfish, Carassius auratus. J. Fish Biol., 1990; 36: 821-831.
14. Blaxhall, P.C.: The haematological assessment of the health of freshwater fish. J. Fish Biol., 1972; 4: 593-604.
15. Tanyer, G.: Hematoloji ve Laboratuvar Ders Kitabı. Ayyıldız Ltd., Ankara, Turkey, 1985: 109-148.
16. Schreck, C.B., Moyle, P.B.: Methods for Fish Biology. American Fisheries Society. Exon Company. Maryland, 1990; 273-322.
17. Seeley, K.R., Gillespie, P.D., Weeks, B.A.: A simple technique for the rapid spectrophotometric determination of phagocytosis by fish macrophages. Mar. Environ. Res., 1990; 30: 37-41.
18. Hayran, M., Özdemir, O.: Bilgisayar, İstatistik ve Tıp. 2. Baskı, Hekimler Yayın Birliği Medikal Araştırma Grubu (MEDAR), Ankara, Turkey, 1996.
19. Keleş, O., Candan, A., Bakırel, T., Karataş, S.: The investigation of the anabolic efficiency and effect on the non-specific immune system of zeranol in rainbow trout (Oncorhynchus mykiss, Walbaum). Turk. J. Vet. Anim. Sci., 2002; 26: 925-931. (article in Turkish, with an abstract in Eglish)
20. Duncan, P.L., Klesius, P.H.: Dietary immunostimulants enhance nonspecific immune responses in channel catfish but not resistance to Edwardsiella ictaluri. J. Aquat. Anim. Health, 1996; 8: 241-248.
21. Rairakhwada, D., Pal, A.K., Bhathena, Z.P., Sahu, N.P., Jha, A., Mukherjee, S.C.: Dietary microbial levan enhances cellular nonspecific immunity and survival of common carp (Cyprinus carpio) juveniles. Fish Shellfish Immunol., 2007; 22: 477-486.
22. Uluköy, G., Timur, M.: A study on observation of haematological and histopathological changes occurred from some pesticides in different concentrations in pikeperch (Stizostedion lucioperca L. 1758). J. Fish. Aquat. Sci., 1993; 10: 37-39. (article in Turkish, with an abstract in English)
23. Robbins, S.L., Kumar, V.: Basic Pathology. 4th edn., W.B. Saunders Comp., 1990.