Effects of supplemental fructo-oligosaccharide and mannanoligosaccharide on nutrient digestibilities, volatile fatty acid concentrations, and immune function in horses

Bu araştırma, atların yemlerine katılan frukto-oligosakkarit (FOS) ve mannan-oligosakkaritin (MOS) besin maddelerinin sindirilme dereceleri, dışkı pH’sı ve uçucu yağ asitleri kompozisyonu ile immun sistem üzerine etkilerini incelemek amacıyla yapılmıştır. Araştırma 4×4 Latin kare deneme düzenine göre yürütülmüş ve değerlendirilmiştir. Dört dönem halinde yürütülen araştırmanın, her bir dönemi 12 günü adaptasyon, 6 günü dışkı ve 2 günü kan numunesi toplama olmak üzere 20’şer gün, toplam 80 gün sürdürülmüştür. Her dönemde dört gruptan biri kontrol olarak tutulmuş, diğer gruplar ise, rasyona 30 g/gün FOS ilave edilen grup, 30 g/gün MOS ilave edilen grup ve 15 g/gün FOS + 15 g/gün MOS ilave edilen grup şeklinde oluşturulmuştur. Dışkı pH’sı ve uçucu yağ asitleri kompozisyonları arasındaki farklılıklar önemli bulunmamıştır (P > 0,05). Gruplara göre besin maddelerinin sindirilme dereceleri arasında farklılıklar önemli bulunmamıştır (P > 0,05). Serum IgA, IgG ve IgM konsantrasyonlarında da önemli bir değişiklik oluşmamıştır (P > 0,05). Ergin atların rasyonlarına FOS, MOS veya FOS + MOS ilavesinin bağırsak sağlığı ve immun sistem üzerinde etkisi bulunmamıştır.

Atlarda frukto-oligosakkarit ve mannan-oligosakkarit kullanımının besin maddelerinin sindirilme dereceleri, uçucu yağ asitleri ile immun sistem üzerine etkileri

This research was performed to study the effects of fructo-oligosaccharide (FOS) and mannan-oligosaccharide (MOS) on nutrient digestibilities, fecal pH and volatile fatty acids compositions, and immune function in adult horses. Four adult Thoroughbred horses were used in a 4×4 Latin Square design with 20-day treatment periods. A 12-day adaptation phase was followed by 6-day collection of feces and 2-day blood sampling phases. Four different treatments were administered: 1) Control (no supplemental FOS or MOS; 2) 30 g of FOS/d; 3) 30 g of MOS/d; 4) 15 g of FOS+15 g of MOS/d. There were no differences among treatments in the fecal pH and volatile fatty acids compositions (P > 0.05). Nutrient digestibilities did not differ among treatments (P > 0.05). Also, no differences were found in the IgA, IgG, and IgM levels among the treatments (P > 0.05). Fructo-oligosaccharides, MOS, or FOS+MOS supplementation did not affect gut health and immune system in adult horses compared to control.

___

  • 1. Sangeetha, P.T., Ramesh, M.N., Prapulla, S.G.: Production of fructo-oligosaccharides by fructosyl transferase from Aspergillus oryzae CFR 202 and Aureobasidium pullulans CFR 77. Process Biochem., 2004; 39: 755-760.
  • 2. Perrin, S., Warchol, M., Grill, J.P., Schneider, F.: Fermentations of fructo-oligosaccharides and their components by Bifidobacterium infantis ATCC 15697 on batch culture in semisynthetic medium. J. Appl. Microbiol., 2001; 90: 859-865.
  • 3. Flickinger, E.A., Van Loo, J., Fahey, G.C. Jr.: Nutritional responses to the presence of inulin and oligofructose in the diets of domesticated animals: a review. Crit. Rev. Food Sci. Nutr., 2003; 43: 19–60.
  • 4. Franklin, S.T., Newman, M.C., Newman, K.E., Meek, K.I.: Immune parameters of dry cows fed mannan oligosaccharide and subsequent transfer of immunity to calves. J. Dairy Sci., 2005; 88: 766-775.
  • 5. Flemming, J.S., Freitas, J.R.S., Fontoura, P., Montanhini Neto, R., Arruda, J.S.: Use of mannanoligosaccharides in broiler feeding. Rev. Bras. Cienc. Avic., 2004; 6: 159-161.
  • 6. Davis, M.E., Maxwell, C.V., Kegley, E.B., de Rodas, B.Z., Friesen, K.G., Hellwig, D.H., Dvorak, R.A.: Efficacy of mannan oligosaccharide (Bio-Mos) addition at two levels of supplemental copper on performance and immunocompetence of early weaned pigs. J. Anim. Sci., 1999; 77 (Suppl. 1): 63 (Abs.).
  • 7. Davis, M.E., Brown, D.C., Maxwell, C.V., Johnson, Z.B., Dvorak, R.A.: Efficacy of mannan oligosaccharide (Bio-Mos) as a complete or partial replacement for zinc oxide in the diets of weanling pigs. J. Anim. Sci., 2001; 79 (Suppl. 2): 78.
  • 8. Harris, P.A.: Comparison of the digestible energy (DE) and net energy (NE) systems for the horse. In: Pagan, J.D., Ed. Advances in Equine Nutrition. Nottingham University Press, United Kingdom. 2001; 199-215.
  • 9. NRC-National Research Council: Nutrient Requirements for Horses, 5th edn., National Academy Press, Washington, DC. 1989.
  • 10. Berg, E.L., Fu, C.J., Porter, J.H., Kerley, M.S.: Fructooligosaccharide supplementation in the yearling horse: effects on fecal pH, microbial content and volatile fatty acid concentrations. J. Anim. Sci., 2005; 83: 1549-1553.
  • 11. AOAC: Official Methods of Analysis. 14th edn. Assoc. Offic. Anal. Chem., Gaithersburg, MD. 1984; 152-159.
  • 12. Goering, H.K., Van Soest, P.J.: Forage Fiber Analyses (Apparatus, Reagents and Some Applications). US Department of Agriculture Handbook, No.379 ARS-USDA, Washington, DC. 1970.
  • 13. Otto, E.R., Yokoyama, M., Hengemuehle, S., von Bermuth, R.D., van Kempen, T., Trottier, N.L.: Ammonia, volatile fatty acids, phenolics, and odor offensiveness in manure from growing pigs fed diets reduced in protein concentration. J. Anim. Sci., 2003; 81: 1754-1763.
  • 14. SPSS: SPSS / PC + V.2.0. Base manual for the IBM PC/XT/AT and PS/2, Marjia and Morusis, 14 Inc. 444 N. Michigan Avenue, Chicago, IL, 60611. 1998.
  • 15. Diez, M., Hornick, J.L., Baldwin, P., Istasse, L.: Influence of a blend of fructo-oligosaccharides and sugar beet fiber on nutrient digestibility and plasma metabolite concentrations in healthy beagles. Am. J. Vet. Res., 1997; 58: 1238-1242.
  • 16. Houdijk, J.G.M., Bosch, M.W., Tamminga, S., Verstegen, W.A., Berenpas, E.B., Knoop, H.: Apparent ileal and total-tract nutrient digestion by pigs as affected by dietary non-digestible oligosaccharides. J. Anim. Sci., 1999; 77: 148–158.
  • 17. Swanson, K.S., Grieshop, C.M., Flickinger, E.A., Bauer, L.L., Healy, H.P., Dawson, K.A., Merchen, N.R., Fahey, G.C. Jr.: Supplemental fructooligosaccharides and mannanoligosaccharides influence immune function, ileal and total tract nutrient digestibilities, microbial populations and concentrations of protein catabolites in the large bowel of dogs. J. Nutr., 2002; 132: 980-989.
  • 18. Propst, E.L., Flickinger, E.A., Bauer, L.L., Merchen, N.R., Fahey, G.C. Jr.: A dose-response experiment evaluating the effects of oligofructose and inulin on nutrient digestibility, stool quality, and fecal protein catabolites in healthy adult dogs. J. Anim. Sci., 2003; 81: 3057-3066.
  • 19. Hussein, H.S., Vogedes, L.A., Fernandez, G.C.J., Frankeny, R.L.: Effects of cereal grain supplementation on apparent digestibility of nutrients and concentrations of fermentation end-products in the feces and serum of horses consuming alfalfa cubes. J. Anim. Sci., 2004; 82: 1986-1996.
  • 20. Campbell, J.M., Fahey. G.C. Jr., Wolf, B.W.: Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal shortchain fatty acids, pH, and microflora in rats. J. Nutr., 1997; 127: 130-136.
  • 21. Mikkelsen, L.L., Jakobsen, M., Jensen, B.B.: Effects of dietary oligosaccharides on microbial diversity and fructooligosaccharide degrading bacteria in faeces of piglets postweaning. Anim. Feed Sci. Technol., 2003; 109: 133-150.
  • 22. Smiricky-Tjardes, M.R., Flickinger, E.A., Grieshop, C.M., Bauer, L.L., Murphy, M.R., Fahey, G.C. Jr.: In vitro fermentation characteristics of selected oligosaccharides by swine fecal microflora. J. Anim. Sci., 2003; 81: 2505-2514.
  • 23. Howard, M.D., Liu, H., Spencer, J.D., Kerley, M.S., Allee, G.L.: Incorporation of short-chain fructooligosaccharides and tylan into diets of early weaned pigs. J. Anim. Sci., 1999; 77 (Suppl. 1): 63 (Abs.).
  • 24. Spearman, K.R.: Effect of mannan oligosaccharide (MOS) supplementation on the immune status of mares and their foals. MSc Thesis, University of Florida, 2004.
  • 25. Franklin, S.T., Newman, K.E., Newman, M.C.: Evaluation of mannan oligosaccharide on the immune status of dairy cows and their calves. J. Anim. Sci., 2002; 80 (Suppl): 192.
  • 26. Grieshop, C.M., Flickinger, E.A., Bruce, K.J., Patil, A.R., Czarnecki-Maulden, GL., Fahey, G.C. Jr.: Gastrointestinal and immunological responses of senior dogs to chicory and mannan-oligosaccharides. Arch. Anim. Nutr., 2004; 58: 483- 493.
  • 27. Davis, M.E., Maxwell, C.V., Brown, D.C., de Rodas, B.Z., Johnson, Z.B., Kegley, E.B., Hellwig, D.H., Dvorak, R.A.: Effect of dietary mannan oligosaccharide and (or) pharmacological additions of copper sulfate on growth performance and immunocompetence of weanling and growing/finishing pigs. J. Anim. Sci., 2002; 80: 2887–2894.
  • 28. Raymond, S.L., Smith, T.K., Swamy, H.V.L.N.: Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on feed intake, serum chemistry, and hematology of horses, and the efficacy of a polymeric glucomannan mycotoxin adsorbent. J. Anim. Sci., 2003; 81: 2123–2130
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

The effects of dietary ascorbic acid supplementation on collagen and amino acid concentrations in Japanese quails exposed to heat stress

Kamil SEYREK, Hakan ERBAŞ, Mukadder SERTER, Çiğdem YENİSEY

Prepucial urethrostomy performed using the coating technique

Suphi Erdem ACAR, Murat ŞAROĞLU, Defne Joan SADALAK

Frequency and antibiotic susceptibility of Pasteurella multocida and Mannheimia haemolytica isolates from nasal cavities of cattle

Kaan ÖNAT, Serpil KAHYA, K. Tayfun ÇARLI

Prevalence of post parturient haemoglobinuria in buffalo and therapeutic trials with toldimfos sodium and tea leaves in Pakistan

Aneela Zameer DURRANI, Nadeem KAMAL, Abdul Rauf SHAKOORI, Rana Muhammad YOUNUS

Yonca otu ve dane mısır partikül büyüklüklerinin süt ineklerinde süt verimi ve bileşenleri, çiğneme aktivitesi ve rumen pH’sı üzerine etkileri

Mehmet Ali BAL, E. Banu Büyükünal BAL

A case study of the clinical form of Johne’s disease in a heifer

Agnieszka WISZNIEWSKA-LASZCZYCH, Joanna SZTEYN, Aleksandra RUSZCZYNSKA

Influence of b-1,3/1,6 glucan applications on some non-specific cellular immune response and haematologic parameters of healthy Nile tilapia (Oreochromis niloticus L., 1758)

Aysel ŞAHAN, Selçuk DUMAN

Determination of microbial contamination sources at a Frankfurter sausage processing line

Ebru GÜNGÖR, Nalan GÖKOĞLU

Influence of β-1,3/1,6 glucan applications on some non-specific cellular immune response and haematologic parameters of healthy Nile tilapia (Oreochromis niloticus L., 1758)

Selçuk DUMAN, Aysel ŞAHAN

β-1,3/1,6 glukan uygulamalarının sağlıklı Nil tilapia (Oreochromis niloticus L., 1758)’larında bazı hematolojik parametreler ve özel olmayan hücresel immun yanıt üzerine etkisi

Aysel ŞAHAN, Selçuk DUMAN