Kamil SEYREK, Hakan ERBAŞ, Mukadder SERTER, Çiğdem YENİSEY

The effects of dietary ascorbic acid supplementation on collagen and amino acid concentrations in Japanese quails exposed to heat stress

A considerable body of data exists regarding the role of vitamin C in mammalian physiology; however, there are no data about the effects of dietary ascorbic acid supplementation on collagen concentrations and amino acid levels in animals exposed to heat stress. The present study investigated the effects of supplementary ascorbic acid intake on collagen concentrations in the brain and heart tissue in Japanese quails. In addition, glycine, glutamine, histidine, asparagine, and serine contents in the livers of the same animals were measured. Japanese quails were allocated into 4 groups, each of which was exposed to heat stress (34.8 ± 1.25 °C) for 75 days. Control animals were fed a basal diet, while animals in the experimental groups were fed a basal diet supplemented with 150, 250, or 500 mg of L-ascorbic acid kg-1 of diet. Compared to the control group, mean collagen concentration in brain tissue significantly (P < 0.05) increased only in Japanese quails given 250 mg of L-ascorbic kg-1 of diet. On the other hand, heart tissue collagen content in the quails fed vitamin C did not significantly increase; in fact, the collagen content in the group fed 500 mg of L-ascorbic acid kg-1 of diet significantly decreased (P < 0.01). Amino acid content in the liver significantly increased in the group fed 150 mg of L-ascorbic acid kg-1 of diet (P < 0.01 for serine and P < 0.001 for the others). In conclusion, vitamin C had profound effects on collagen synthesis and amino acid metabolism in Japanese quails subjected to heat stress. Results of the present study also indicate that addition of high-dose dietary vitamin C-higher than 250 mg kg-1 of diet-may have detrimental effects in quails exposed to heat stress.

Anahtar Kelimeler:

Key words: Reactive oxygen radicals, ascorbic acid, quail, collagen, amino acid

The effects of dietary ascorbic acid supplementation on collagen and amino acid concentrations in Japanese quails exposed to heat stress

Keywords:

Key words: Reactive oxygen radicals, ascorbic acid, quail, collagen, amino acid,

PDF

___

Mukhopadhyay, C.K., Chatterjee, I.B.: Free metal ion- independent oxidative damage of collagen. Protection by ascorbic acid. J. Biol. Chem., 1994; 269: 30200-30205.
Aragão, C., Corte-Real, J., Costas, B., Dinis, M.T., Conceicão, L.E.C.: Stress response and changes in amino acid requirements in Senegalese sole (Solea senegalensis Kaup 1858). Amino Acids, 2008; 34: 143-148.
Tavares, D.C., Cecchi, A.O., Antunes, L.M.G., Takahashi, C.S.: Protective effects of the amino acid glutamine and ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells. Teratog. Carcinog. Mutagen., 1998; 18: 153- 161.
Reddy, G.K., Enwemeka, C.S.: A simplified method for the analysis of hydroxyproline in biological tissues. Clin. Biochem., 1996; 29: 225-229.
Yenisey, C., Seyrek, K., Serter, M., Kargin Kiral, F., Bardakcioglu, H.E.: Effects of dietary vitamin C supplementation on glutathione, malondialdehyde and nitric oxide concentrations in brain and heart of laying Japanese quails exposed to heat stress (34.8 °C). Rev. Méd. Vét., 2006; 157: 149-152.
Sienkiewicz, P., Pałka, M., Pałka, J.: Oxidative stress inducess IGF-I receptor signaling disturbances in cultured human dermal fibroblats. A possible mechanism for collagen biosynthesis inhibition. Cell. Mol. Biol. Lett., 2004; 9: 643-650.
Günel, E., Çağlayan, F., Çağlayan, O., Canbilen, A., Tosun, M.: Effects of antioxidant therapy on collagen synthesis in corrosive esophageal burns. Pediatr. Surg. Int., 2002; 18: 24-27.
Stuart, E.L., Evans, G.S., Lin, Y.S., Powers, H.J.: Reduced collagen and ascorbic acid concentrations and increased proteolytic susceptibility with prelabor fetal membrane rupture in women. Biol. Reprod., 2005; 72: 230-235.
MacDermott, R.I.J., Landon, C.R.: The hydroxyproline content of amnion and prelabour rupture of the membranes. Eur. J. Obstet. Gyn. R. B., 2000; 92: 217-221.
Meneghini, R.: Genotoxicity of active oxygen species in mammalian cells. Mutat. Res., 1988; 195: 215-230.
Siwik, D.A., Pagano, P.J., Colucci, W.S.: Oxidative stress regulates collagen synthesis and matrix metalloproteinase activity in cardiac fibroblast. Am. J. Physiol. Cell Physiol., 2001; 280: 53-60.
Bergström, J., Fuerst, P., Norée, L.O., Vinnars, E.: Intracellular free amino acid concentration in human muscle tissue. J. Appl. Physiol., 1974; 36: 693-697.