Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media

The aim of this study was to determine the optimal dose of EGF on in vitro maturation of bovine oocytes and to investigate the effect of different IVF and IVC media on development of bovine embryos matured with EGF. Oocytes collected from a slaughterhouse were randomly divided into 4 groups. Each group of oocytes was incubated with different doses of EGF (Control: 0 ng/mL, Group 1: 1 ng/mL, Group 2: 10 ng/mL, and Group 3: 100 ng/mL) for 24 h to determine the optimal dose. Matured oocytes were fertilized with frozen-thawed bull sperm by using the swim-up method. The oocytes in each group were divided into 2 subgroups and cultured in CR1aa or G1.3/G2.3 media. Embryos were evaluated for cleavage (48th h), and 5-8 cell embryo (day 3) and blastocyst development (day 8) after fertilization. The rates of cumulus expansion and nuclear maturation were higher (P < 0.01) in GI, GII, and GIII compared to the control group. However, no significant difference was observed on oocyte maturation rates between different doses of EGF. Cleavage rates were higher (P < 0.01) in EGF groups than in the control group. No significant differences were obtained in blastocyst development between CR1aa and G1.3/G2.3 media (P > 0.1).

Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media

The aim of this study was to determine the optimal dose of EGF on in vitro maturation of bovine oocytes and to investigate the effect of different IVF and IVC media on development of bovine embryos matured with EGF. Oocytes collected from a slaughterhouse were randomly divided into 4 groups. Each group of oocytes was incubated with different doses of EGF (Control: 0 ng/mL, Group 1: 1 ng/mL, Group 2: 10 ng/mL, and Group 3: 100 ng/mL) for 24 h to determine the optimal dose. Matured oocytes were fertilized with frozen-thawed bull sperm by using the swim-up method. The oocytes in each group were divided into 2 subgroups and cultured in CR1aa or G1.3/G2.3 media. Embryos were evaluated for cleavage (48th h), and 5-8 cell embryo (day 3) and blastocyst development (day 8) after fertilization. The rates of cumulus expansion and nuclear maturation were higher (P < 0.01) in GI, GII, and GIII compared to the control group. However, no significant difference was observed on oocyte maturation rates between different doses of EGF. Cleavage rates were higher (P < 0.01) in EGF groups than in the control group. No significant differences were obtained in blastocyst development between CR1aa and G1.3/G2.3 media (P > 0.1).

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Bibtex @ { tbtkveterinary150892, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2010}, volume = {34}, pages = {33 - 38}, doi = {}, title = {Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media}, key = {cite}, author = {Baştan, Ayhan and Polat, Bülent and Acar, Duygu BAKİ and Korkmaz, Ömer and Çolak, Armağan} }
APA Baştan, A , Polat, B , Acar, D , Korkmaz, Ö , Çolak, A . (2010). Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media . Turkish Journal of Veterinary and Animal Sciences , 34 (1) , 33-38 .
MLA Baştan, A , Polat, B , Acar, D , Korkmaz, Ö , Çolak, A . "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media" . Turkish Journal of Veterinary and Animal Sciences 34 (2010 ): 33-38 <
Chicago Baştan, A , Polat, B , Acar, D , Korkmaz, Ö , Çolak, A . "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media". Turkish Journal of Veterinary and Animal Sciences 34 (2010 ): 33-38
RIS TY - JOUR T1 - Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media AU - Ayhan Baştan , Bülent Polat , Duygu BAKİ Acar , Ömer Korkmaz , Armağan Çolak Y1 - 2010 PY - 2010 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 33 EP - 38 VL - 34 IS - 1 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media %A Ayhan Baştan , Bülent Polat , Duygu BAKİ Acar , Ömer Korkmaz , Armağan Çolak %T Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media %D 2010 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 34 %N 1 %R %U
ISNAD Baştan, Ayhan , Polat, Bülent , Acar, Duygu BAKİ , Korkmaz, Ömer , Çolak, Armağan . "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media". Turkish Journal of Veterinary and Animal Sciences 34 / 1 (Ocak 2010): 33-38 .
AMA Baştan A , Polat B , Acar D , Korkmaz Ö , Çolak A . Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turkish Journal of Veterinary and Animal Sciences. 2010; 34(1): 33-38.
Vancouver Baştan A , Polat B , Acar D , Korkmaz Ö , Çolak A . Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turkish Journal of Veterinary and Animal Sciences. 2010; 34(1): 33-38.
IEEE A. Baştan , B. Polat , D. Acar , Ö. Korkmaz ve A. Çolak , "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media", Turkish Journal of Veterinary and Animal Sciences, c. 34, sayı. 1, ss. 33-38, Oca. 2010
Turkish Journal of Veterinary and Animal Sciences
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

61.6b55.9b

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