In vitro culture of in vivo Saanen goat embryos by vitrification
In vitro culture of in vivo Saanen goat embryos by vitrification
The purpose of this study was to investigate the survival rate of high-quality embryos (n: 101) derived in vivo from Saanen goats (n: 15) in culture following their vitrified freezing and thawing during the breeding season. The in vitro postthaw survival rates of 101 vitrified embryos at 24, 48, and 72 h were 59.4%, 33.6%, and 25.7%, respectively. According to the developmental stage of the embryos, the survival rates were 51.3%, 20.5%, and 15.4% in the morula, and 64.5%, 41.9%, and 32.3% in the blastocyst, respectively. The survival rates of the blastocysts and morulae at 48 and 72 h were significantly different (P < 0.05). While the survival rates of the embryos of Grade 1 quality at 24, 48, and 72 h were 78.6%, 46.4%, and 32.1%, Grade 2 quality survival rates were 35.5%, 17.8%, and 17.8%, respectively. The survival rates of Grades 1 and 2 embryos at 24 h (P < 0.001) and 48 h (P < 0.05) were significantly different. As a result, it was concluded that the embryos to be frozen should be selected according to their development stage and quality, and preferably should be of Grade 1 quality in the blastocyst stage for the cryopreservation of in vivo-derived goat embryos by vitrification.
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- 1. Kaymaz M. Yardımcı üreme teknikleri. In: Semacan A, Kaymaz M, Fındık M, Rişvanlı A, Köker A, editors. Çiftlik Hayvanlarında Doğum ve İnfertilite. 1st ed. Malatya, Turkey: Medipres; 2012. pp. 695-811.
- 2. Pereira RM, Marques CC. Animal oocyte and embryo cryopreservation. Cell Tissue Bank 2008; 9: 267-277.
- 3. Gama LT, Bressan MC. Biotechnology applications for the sustainable management of goat genetic resources. Small Rumin Res 2011; 98: 133-146.
- 4. Amiridis GS, Cseh S. Assisted reproductive technologies in the reproductive management of small ruminants. Anim Reprod Sci 2012; 130: 152-161.
- 5. Verma OP, Kumar R, Kumar A, Chard S. Assisted reproductive techniques in farm animal from artificial insemination to nanobiotechnology. Vet World 2012; 5: 301-310.
- 6. Vajta G. Vitrification of the oocytes and embryos of domestic animals. Anim Reprod Sci 2000; 60-61: 357-364.
- 7. Massip A. Cryopreservation of embryos of farm animals. Reprod Dom Anim 2001; 36: 49-55.
- 8. Sağırkaya H, Bağış H. Memeli embriyolarının kriyoprezervasyonu. Uludağ Univ J Fac Vet Med 2003; 22: 127-135.
- 9. Kanagawa H, Shimohira I, Saitoh N. Manual of Bovine Embryo Transfer. 1st ed. Japan: Japan Livestock Technology Association; 1995. pp. 259-315.
- 10. Uysal O. Sığır embriyolarının vitrifikasyonu. Vet Hekim Der Derg 2007; 78: 45-50.
- 11. Aminoor Rahman AN, Abdullah R, Wan Khadijah WE. A review of reproductive biotechnologies and their application in goat. Biotechnology 2008; 7: 371-384.
- 12. Gibbons A, Cueto M. Embryo Transfer in Sheep and Goats. Buenos Aires, Argentina: Bariloche Experimental Station. National Institute for Agricultural Technology; 2011. pp. 1-33.
- 13. Vajta G, Kuwayama M. Improving cryopreservation systems. Theriogenology 2006; 65: 236-244.
- 14. Gajda B, Smorag Z. Oocyte and embryo cryopreservation - State of art and recent developments in domestic animals. J Anim Feed Sci 2009; 18: 371-387.
- 15. Noakes ED, Parkinson TJ, England GCW, Arthur GH. Embryo transfer in large domestic animals. In: Noakes ED, Parkinson TJ, England GCW, Arthur GH, editors. Arthurs Veterinary Reproduction and Obstetrics. 8th ed. Oxford, UK: W.B. Saunders; 2001. pp. 819-836.
- 16. Cognie Y, Baril G, Poulin N, Mermillod P. Current status of embryo technologies in sheep and goat. Theriogenology 2003; 59: 171-188.
- 17. Flores-Foxworth G. Reproductive biotechnologies in the goat. In: Yongquist RS, Threlfall WR, editors. Current Therapy in Large Animal Theriogenelogy. 2nd ed. St. Louis, MO, USA: W.B. Saunders; 2007. pp. 603-614.
- 18. Saragusty J, Arav A. Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction 2011; 141: 1-19.
- 19. Palasz AT, Mapletoft RJ. Cryopreservation of mammalian embryos and oocytes: recent advances. Biotechnol Adv 1996; 14: 127-149.
- 20. Prentice JR, Anzar M. Cryopreservation of mammalian oocyte for conservation of animal genetics. Vet Med Int 2011: 1-11.
- 21. Fahning ML, Garcia MA. Status of cryopreservation of embryos from domestic animals. Cryobiology 1992; 29: 1-18.
- 22. Holtz W. Recent developments in assisted reproduction in goats. Small Rumin Res 2005; 60: 95-110.
- 23. Morato R, Romaguera R, Izquierdo D, Paramio MT, Mogas T. Vitrification of in vitro produced goat blastocysts: effects of oocyte donor age and development stage. Cryobiology 2011; 63: 240-244.
- 24. Al Yacoub AN, Gauly M, Holtz W. Open pulled straw vitrification of goat embryos at various stages of development. Theriogenology 2010; 73: 1018-1023.
- 25. Ağaoğlu AR, Karakaş K, Kaymaz M. In vivo embryo production in some native goat breed in Turkey. Turk J Vet Anim Sci 2014; 38: 238-244.
- 26. Rao VH, Sarmah BC, Agrawal KP, Ansari MR, Bhattacharya NK. Survival of goat embryos frozen and thawed rapidly. Anim Reprod Sci 1988; 16: 261-264.
- 27. Huang JC, Lin HH, Wu JS, Tang PH, Wang DG, Liu BT. Vitrification of caprine embryos in microdrops. In: Symposium COA/INRA Scientific Cooperation in Agriculture. Tainan, Taiwan (R.O.C): 710 November 2006. pp. 47-58.
- 28. Gal FL, Baril G, Vallet JC, Leboeuf B. In vivo and in vitro survival of goat embryos after freezing with ethylene glycol or glycerol. Theriogenology 1993; 40: 771-777.
- 29. Martemucci G, DAlessandro AG. Efficiency of FSH/LH treatments for in vivo production of embryos and their cryopreservation by different methods in goats. Small Rumin Res 2013; 114: 264-271.
- 30. Tekeli T. Embriyo nakli. In: Alaçam E, editor. Evcil Hayvanlarda Doğum ve İnfertilite. 5th ed. Ankara, Turkey: Medisan; 2005. pp. 81-97.