Effect of retinol and retinol esters on performance, egg quality, and blood and egg vitamin A levels in laying quails

This study aimed to determine the effects of retinol and retinol ester supplementation on performance, egg quality, serum and egg oxidative status, and vitamin A levels in laying quails. Three hundred quails were divided into 5 groups. The control group received a basal diet that contained vitamin premix without vitamin A or vitamin A precursors. The treatment groups received dietary supplementation with retinol and retinol esters [retinyl acetate (R-acetate), retinyl palmitate (R-palmitate), retinyl propionate (R-propionate), 3300 IU/kg]. Egg production and feed conversion ratio improved in all experimental groups. Serum β-carotene and malondialdehyde (MDA) levels decreased and serum retinol levels increased in all experimental groups. Yolk color in the 8th week decreased in the R-propionate group. Serum antioxidant activity levels increased in the R-palmitate and R-propionate groups. Yolk retinol levels increased in the R-palmitate and R-acetate groups. Yolk MDA level decreased in the retinol, R-acetate, and R-propionate groups on the 8th and 30th days and in the retinol and R-propionate groups on the 15th day of storage.In conclusion, the supplementation of laying quail diets with retinol and retinol esters affects performance and egg quality positively, while egg vitamin A and oxidative status differ depending on variations in dietary retinol and retinol ester supplementation.

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1. McDowell P. Vitamins in Animal Nutrition: Vitamin A. San Diego, CA, USA: Academic Press, Inc.; 1998.
2. Lin H, Wang LF, Song JL, Xie YM, Yang QM. Effect of dietary supplemental levels of vitamin A on the egg production and immune responses of heat-stressed laying hens. Poultry Sci 2002; 81: 458-465.
3. West CE, Sijtsma SR, Peters HP, Rombout JH, Van Der Zijpp AJ. Production of chickens with marginal vitamin A deficiency. Br J Nutr 1992; 68: 283-291.
4. Ayaşan T, Karakozak E. Use of β-carotene in animal nutrition and its effects. Kafkas Univ Vet Fak Derg 2010; 16: 697-705.
5. Sevimli A, Yağcı A, Bülbül A. Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy. Biotech Histochem 2013; 88: 336-344.
6. Sevimli A, Bülbül T, Bülbül A, Yağcı A. Chicken amyloid arthropathy: serum amyloid A, interleukin-1β, interleukin-6, tumour necrosis factor -α and nitric oxide profile in acute phase (12th hour). Pol J Vet Sci 2013; 16: 241-247.
7. Blair R. Nutrition and Feeding of Organic Poultry. Wallingford, UK: CAB International; 2008.
8. NRC. Nutrient Requirements of Poultry. 9th ed. Washington, DC, USA: National Academy Press; 1994.
9. Fu Z, Kato H, Sugahara K, Kubo T. Retinoic acid accelerates the development of reproductive organs and egg production in Japanese quail (Coturnix coturnix japonica). Biol Reprod 2000; 63: 1795-1800.
10. Mendonca CX, Almeida CRM, Watanabe C. Effect of dietary vitamin A on egg yolk retinol and tocopherol levels. J Appl Poult Res 2002; 11: 373-378.
11. Mori AV, Mendonca CX, Almeida CRM, Pita MCG. Supplementing hen diets with vitamins A and E affects egg yolk retinol and α-tocopherol levels. J Appl Poult Res 2003;12: 106-114.
12. Ramalho HMM, Dias Da Silva KH, Alves Dos Santos VV, Dos Santos Cavalcanti J, Dimenstein R. Effect of retinylpalmitate supplementation on egg yolk retinol and cholesterol concentrations in quail. Brit Poultry Sci 2008; 49: 475-481.
13. Leeson S, Summers JD. Nutrition of the Chicken. Guelph, Canada: University Books; 2001.
14. Frolik CA, Olson JA. Extraction, separation, and chemical analysis of retinoids. In: Sporn MB, Roberts AB, Goodman DS, editors. The Retinoids. Vol. 1. Orlando, FL, USA: Academic Press, Inc.; 1984. pp. 181-233.
15. Suzuki JP, Katoh NA. A simple and cheap method for measuring serum vitamin in cattle using only spectrophotometer. Jpn J Vet Sci 1990; 52:1281-1283.
16. Draper H, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186: 421-430.
17. Kanner J, Rosenthli I. An assessment of lipid oxidation in foods. Pure Appl Chem 1992; 64: 1959-1964.
18. Koracevic D, Koracevic G, Djordjevic V, Andrejevic V, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001; 54: 356-361.
19. Uni Z, Zaiger G, Gal-Garber O, Pines M, Rozenboim I, Reifen R. Vitamin A deficiency interferes with proliferation and maturation of cells in the chicken small intestine. Brit Poultry Sci 2000; 41: 410-415.
20. Coşkun B, Inal F, Celik I, Erganiş O, Tiftik AM, Kurtoğlu F, Kuyucuoğlu Y, Ok U. Effects of dietary levels of vitamin A on the egg yield and immune response of laying hens. Poultry Sci 1998; 77: 542-546.
21. Marques RH, Gravena RA, Da Silva JDT, Roccon J, Picarelli J, Hada FH, De Queiroz SA, Moraes MB. Effect of supplementation of diets for quails with vitamins A, D and E on performance of the birds and quality and enrichment of eggs. R Bras Zootec 2011; 40: 1222-1232.
22. Goodwin TW. Biochemistry of the Carotenoids. 2nd ed. New York, NY, USA: Chapman and Hall; 1980.
23. Surai PF, Ionov IA, Kuklenko TV, Kostjuk IA, Macpherson A, Speake BK, Noble RC, Sparks NHC. Effect of supplementing the hens diet with vitamin A on the accumulation of vitamins A and E, ascorbic acid and carotenoids in the egg yolk and in the embryonic liver. Brit Poultry Sci 1998; 39: 257-263.
24. Murray RK, Granner DK, Mayes PA, Rodwell VW. Structure and function of the lipid-soluble vitamins. In: Mayes PA, editor. Harpers Biochemistry. 23rd ed. East Norwalk, CT, USA: Appleton & Lange; 1993. pp. 589-601.
25. Joshi PS, Murthy SN, Murthy SK, Ganguly J. Vitamin A economy of the developing embryo and of the freshly hatched chick. Biochem J 1973; 136: 757-761.
26. Hill EW, Scott ML, Norris LC, Henser GF. Reinvestigation of the vitamin A and D requirements of laying and breeding hens and their progeny. Poultry Sci 1961; 40: 1245-1247.
27. Sies H, Stahl W, Sundquist AR. Antioxidant functions of vitamins, vitamins E and C, β-carotene and other carotenoids. Ann NY Acad Sci 1992; 669: 7-20.
28. Kucuk O, Sahin N, Sahin K. Supplemental zinc and vitamin A can alleviate negative effects of heat stress in broiler chickens. Biol Trace Elem Res 2003; 94:225-235.
29. Zhang GF, Yang ZB, Wang Y, Yang WR, Jiang SZ, Gai GS. Effects of ginger root (Zingiber officinale) processed to different particle sizes on growth performance, antioxidant status, and serum metabolites of broiler chickens. Poultry Sci 2009; 88: 2159-2166.
30. Yesilbag D, Gezen SS, Biricik H, Bulbul T. Effect of a rosemary and oregano volatile oil mixture on performance, lipid oxidation of meat and haematological parameters in Pharaoh quails. Brit Poultry Sci 2012; 53: 89-97.