Seyfullah HALİLOĞLU, Zafer BULUT, Behiç SERPEK, Nuri BAŞPINAR, Hüseyin ERDEM

Holstein ırkı gebe ineklerin plaza ve ovaryum vitamin C, progesteron ve 17 beta-östradiol düzeyleri arasındaki ilişkiler

The relationship between ascorrbic acid, oestradiol 17 beta and progesterone in plasma and ovaries in pregnant holstein cows

This study was performed to determine the relationship between the corpus luteum weight/diameter, follicular diameter and the levels of vitamin C, progesterone and oestradiol 17-b of the plasma, corpus luteum and follicular fluid of pregnant Holstein cows in a slaughterhouse. Progesterone and oestradiol 17-b concentrations were determined by the microtitration plate EIA method. Vitamin C levels were determined by spectrophotometer. Although there was a positive correlation between follicular fluid oestradiol 17-b levels and the follicle diameter in the follicular fluid, in contrast to oestradiol 17-b levels, small follicles have higher progesterone content than large follicles. This negative correlation was due to the fact that progesterone is the precursor of oestradiol 17-b. There was no correlation between corpus luteum progesterone levels and corpus luteum weight/diameter. However, a positive correlation between corpus luteum progesterone levels and the corpus luteum weight/diameter was observed; therefore, we suggest that this situation resulted from placenta-originated progesterone synthesis in pregnancy. Furthermore, levels of corpus luteum vitamin C were found to be higher and we concluded that this increase resulted from the effect of vitamin C on steroid and collagen biosynthesis. Nevertheless, negative correlations between corpus luteum vitamin C concentrations and corpus luteum weight/diameter were seen, and hence we suggest that this may be the result of the inhibitory effect of vitamin C on PGF2a synthesis.

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