Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats

To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.

Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats

To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.

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Bibtex @ { tbtkveterinary152742, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2014}, volume = {38}, pages = {699 - 706}, doi = {10.3906/vet-1404-54}, title = {Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats}, key = {cite}, author = {Navanukraw, Chainarong and Thammasırı, Jiratti and Moonmanee, Tossapol and Natthakornkul, Jaruk} }
APA Navanukraw, C , Thammasırı, J , Moonmanee, T , Natthakornkul, J . (2014). Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats . Turkish Journal of Veterinary and Animal Sciences , 38 (6) , 699-706 . DOI: 10.3906/vet-1404-54
MLA Navanukraw, C , Thammasırı, J , Moonmanee, T , Natthakornkul, J . "Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats" . Turkish Journal of Veterinary and Animal Sciences 38 (2014 ): 699-706 <
Chicago Navanukraw, C , Thammasırı, J , Moonmanee, T , Natthakornkul, J . "Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats". Turkish Journal of Veterinary and Animal Sciences 38 (2014 ): 699-706
RIS TY - JOUR T1 - Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats AU - Chainarong Navanukraw , Jiratti Thammasırı , Tossapol Moonmanee , Jaruk Natthakornkul Y1 - 2014 PY - 2014 N1 - doi: 10.3906/vet-1404-54 DO - 10.3906/vet-1404-54 T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 699 EP - 706 VL - 38 IS - 6 SN - 1300-0128-1303-6181 M3 - doi: 10.3906/vet-1404-54 UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats %A Chainarong Navanukraw , Jiratti Thammasırı , Tossapol Moonmanee , Jaruk Natthakornkul %T Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats %D 2014 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 38 %N 6 %R doi: 10.3906/vet-1404-54 %U 10.3906/vet-1404-54
ISNAD Navanukraw, Chainarong , Thammasırı, Jiratti , Moonmanee, Tossapol , Natthakornkul, Jaruk . "Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats". Turkish Journal of Veterinary and Animal Sciences 38 / 6 (Haziran 2014): 699-706 .
AMA Navanukraw C , Thammasırı J , Moonmanee T , Natthakornkul J . Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats. Turkish Journal of Veterinary and Animal Sciences. 2014; 38(6): 699-706.
Vancouver Navanukraw C , Thammasırı J , Moonmanee T , Natthakornkul J . Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats. Turkish Journal of Veterinary and Animal Sciences. 2014; 38(6): 699-706.
IEEE C. Navanukraw , J. Thammasırı , T. Moonmanee ve J. Natthakornkul , "Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats", Turkish Journal of Veterinary and Animal Sciences, c. 38, sayı. 6, ss. 699-706, Haz. 2014, doi:10.3906/vet-1404-54
Turkish Journal of Veterinary and Animal Sciences
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

61.6b55.9b

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