Survivability of caprine oocytes vitrifed in conventional and open pulled straws

Survivability of caprine oocytes vitrifed in conventional and open pulled straws

The aim of this study was to compare the efficacy of conventional straw vitrifcation with open pulled straw vitrifcation interms of cryosurvivability and damages caused to the oocytes. Two hundred immature goat cumulus oocyte complexes (COCs) werevitrifed in a solution of ethylene glycol, dimethyl sulfoxide, and sucrose using either conventional straws (100 COCs) or open pulledstraws (100 COCs). Te COCs were warmed and observed for morphological damages and viability afer 7 days of preservation inliquid nitrogen. Among the 100 COCs cryopreserved in each case, only 83 COCs were recovered afer warming in conventional strawvitrifcation as compared to 94 COCs in open pulled straw vitrifcation. In terms of morphological survivability, the percentage ofmorphologically normal oocytes was greater (P < 0.01) in the case of open pulled straw vitrifcation (86.2%) as compared to conventionalstraw vitrifcation (59.0%). Viability percentage of live oocytes was greater (P < 0.01) in the case of open pulled straw vitrifcation(90.4%) as compared to conventional straw vitrifcation (66.3%). Te results indicate that open pulled straw vitrifcation is better thanconventional straw vitrifcation for rapid freezing of immature goat COCs in terms of both morphological survival and viability.

___

  • 1. Gupta MK, Lee HT. Cryopreservation of oocytes and embryos by vitrifcation. Korean J Reprod Med 2010; 37: 267–291.
  • 2. Sherman JK, Lin TP. Survival of unfertilized mouse eggs during freezing and thawing. Proc Soc Exp Biol Med 1958; 98: 902–905.
  • 3. Larman MG, Sheehan CB, Gardner DK. Calcium-free vitrifcation reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rate in mouse oocytes. Reproduction 2006; 131: 53–61.
  • 4. Kohaya N, Fujiwra K, Ito J, Kashiwazaki N. Generation of live ofspring from vitrifed mouse oocytes of C57BL/6J strain. PLoS One 2013; 8: e58063.
  • 5. Lee H, Elmoazzen H, Wright D, Biggers J, Rueda BR, Heo YS, Toner M, Toth TL. Ultra-rapid vitrifcation of mouse oocytes in low cryoprotectant concentrations. Reprod Biomed Online 2010; 20: 201–208.
  • 6. Morato R, Izquierdo D, Paramio MT, Mogas T. Cryotops versus open-pulled straws (OPS) as carriers for the cryopreservation of bovine oocytes: efects on spindle and chromosome confguration and embryo development. Cryobiology 2008; 57: 137–141.
  • 7. Zhou XL, Al Naib A, Sun DW, Lonergan P. Bovine oocyte vitrifcation using the Cryotop method: effect of cumulus cells and vitrifcation protocol on survival and subsequent development. Cryobiology 2010; 61: 66–72.
  • 8. Dalvit G, Gutnisky C, Alvarez G, Cetica P. Vitrifcation of bovine oocytes and embryos. Cryobiology 2012; 65: 341–342.
  • 9. Dhali A, Manik RS, Das SK, Singla SK, Palta P. Vitrifcation of bufalo (Bubalus bubalis) oocytes. Teriogenology 2000; 53: 1295–1303.
  • 10. Sharma A, Purohit GN. Vitrifcation of immature bubaline cumulus oocyte complexes by the open-pulled straw and conventional straw methods and their subsequent in vitro fertilization. Vet Med-Czech 2008; 53: 427–433.
  • 11. Liang Y, Rakwongrit D, Somfai T, Nagai T. Parnpai R. Cryopreservation of immature bufalo oocytes: efects of cytochalasin B pretreatment on the efciency of cryotop and solid surface vitrifcation methods. Anim Sci J 2012; 83: 630– 638.
  • 12. Ebrahimi B, Valojerdi MR, Efekhari-Yazdi P, Baharvand H, Farrokhi A. IVM and gene expression of sheep cumulus– oocyte complexes following diferent methods of vitrifcation. Reprod Biomed Online 2010; 20: 26–34.
  • 13. Bhat MH, Yaqoob SH, Khan FA, Waheed SM, Sharma V, Vajta G, Ganai NA, Shah RA. Open pulled straw vitrifcation of in vitro matured sheep oocytes using diferent cryoprotectants. Small Ruminant Res 2013; 112: 136–140.
  • 14. Huang WT, Holtz W. Efects of meiotic stages, cryoprotectants, cooling and vitrifcation on the cryopreservation of porcine oocytes. Asian-Aust J Anim Sci 2002; 15: 485–493.
  • 15. Galeati G, Spinaci M, Vallorani C, Bucci D, Porcu E, Tamanini C. Pig oocyte vitrifcation by cryotop method: efects on viability, spindle and chromosome confguration and in vitro fertilization. Anim Reprod Sci 2011; 127: 43–49.
  • 16. Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efcient vitrifcation method for cryopreservation of human oocytes. Reprod Biomed Online 2005; 11: 300–308.
  • 17. Kuwayama M. Highly efcient vitrifcation for cryopreservation of human oocytes and embryos: the Cryotop method. Teriogenology 2007; 67: 73–80.
  • 18. Edgar DH, Gook DA. A critical appraisal of cryopreservation (slow cooling versus vitrifcation) of human oocytes and embryos. Hum Reprod Update 2012; 18: 536–554.
  • 19. Garg N, Purohit GN. Efect of diferent cryoprotectant concentrations for ultrarapid freezing of immature goat follicular oocytes on their subsequent maturation and fertilization in vitro. Anim Reprod 2007; 4: 113–118.
  • 20. Nema S, Jain SK, Gehlaut BS, Sarkhel BE. Vitrifcation of immature goat oocytes by solid surface vitrifcation technique. Indian J Anim Sci 2007; 77: 556–558.
  • 21. Rao BS, Mahesh YU, Charan KV, Suman K, Sekhar N, Shivaji S. Effect of vitrifcation on meiotic maturation and expression of genes in immature goat cumulus oocyte complexes. Cryobiology 2012; 64: 176–184.
  • 22. Wani NA, Wani GM, Khan MZ, Sidiqi MA. Effect of diferent factors on the recovery rate of oocytes for in vitro maturation and in vitro fertilization procedures in sheep. Small Ruminant Res 1999; 34: 71–76.
  • 23. Naik BR, Rao BS, Vagdevi R, Gnanprakash M, Amarnath D, Rao VH. Conventional slow freezing, vitrifcation and open pulled straw (OPS) vitrifcation of rabbit embryos. Anim Reprod Sci 2005; 86: 329–338.
  • 24. Rao BS, Mahesh YU, Suman K, Charan KV, Lakshmikantan U, Gibence HRW, Shivaji S. Meiotic maturation of vitrifed immature chousingha (Tetracerus quadricornis) oocytes recovered postmortem. Cryobiology 2011; 62: 47–52.
  • 25. Gupta PSP, Nandi S, Ravindranatha BM, Sarma PV. Trypan blue staining to diferentiate live and dead bufalo oocytes and its efect on embryo development in vitro. Bufalo Journal 2002; 18: 321–330.
  • 26. Vajta G, Lewis IM, Kuwayama M, Greve T, Callesen H. Sterile application of the open pulled straw (OPS) vitrifcation method. Cryo-Lett 1998; 19: 389–392.
  • 27. El-Sokary MMM, Kandiel MMM, Mahmoud KGM, Abouel- Roos MEA, Sosa GAM. Evaluation of viability and nuclear status in vitrifed mature bufalo oocytes. Global Veterinaria 2013; 10: 297–302.
  • 28. Chen S, Lien Y, Chen H, Chao K, Ho H, Yang Y. Open pulled straws for vitrifcation of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws. Human Reprod 2000; 15: 2598–2603.
  • 29. Yadav RC, Sharma A, Garg N, Purohit GN. Survival of vitrifed water bufalo cumulus oocyte complexes and their subsequent development in vitro. Bulgarian J Vet Med 2008; 11: 55–64.
  • 30. Purohit GN, Meena V, Solanki K. Morphological survival and subsequent in vitro maturation of denuded and cumulus compact bubaline oocytes cryopreserved by ultra rapid cooling. J Bufalo Sci 2012; 1: 78–83.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Polledness intersex syndrome in goatsmolecular and histological aspects

Iwona SZATKOWSKA, Witold Stanislaw PROSKURA, Sara TABOR, Daniel ZABORSKI

Molecular, cellular, and physiological determinants of bull fertility

Alexis Marie PARISI, Shannon Kate THOMPSON, Abdullah KAYA, Erdoğan MEMİLİ

Study of insulin-like growth factor 1 effects on bovine type A spermatogonia proliferation and viability

Babak QASEMI PANAHI, Parviz TAJIK, Mansoureh MOVAHEDIN, Gholamali MOGHADDAM, Mohammad Hossein GERANMAYEH

Sequencing-based polymorphism in leptin receptor gene and lack of association with postpartum anestrus in Murrah bufaloes

Rajesh KUMAR, Meenakshi GUPTA, Sayed SHAFIQ, Ashok Kumar BALHARA, Sadeesh EM, Mayukh GHOSH, Inderjeet SINGH

Endocrine and metabolic mechanisms of embryo and fetal intrauterine growth retardation in dairy cows

Anatoliy NEZHDANOV, Sergey SHABUNIN, Vitaliy MIKHALEV, Nikolay KLIMOV, Anton CHERNITSKIY

The microRNAs important for ovarian and early embryonic development in cattle

Lei WANG, Jianbo YAO

Radoslava VLCKOVA, Jan POSIVAK, İgor VALOCKY, Zuzana KRAVCOVA, Andrea EIBENOVA, Drahomíra SOPKOVA

Investigation of the efficacy of Tarantula cubensis extract (Theranekron D6) in the treatment of subclinical and clinical mastitis in dairy cows

Kutlay GÜRBULAK, Aytaç AKÇAY, Kadir Semih GÜMÜŞSOY, Birgit SIST

New understanding of an old phenomenon: uncontrolled factors and misconceptions that cast a shadow over studies of the male efect' on reproduction in small ruminants

Trina JORRE DE ST JORRE, Graeme Bruce MARTIN, Penny Alison Rhian HAWKEN

Sequencing-based polymorphism in leptin receptor gene and lack of association with postpartum anestrus in Murrah buffaloes

Rajesh KUMAR, Meenakshi GUPTA, Sayed SHAFIQ, Ashok Kumar BALHARA, Sadeesh EM, Mayukh GHOSH, İnderjeet SINGH