Expression of the GPRC5D gene in Liaoning Cashmere goats

This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence was subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressed only in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere growth.

Expression of the GPRC5D gene in Liaoning Cashmere goats

This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence was subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressed only in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere growth.

___

  • Clark RB. Profile of Brian K Kobilka and Robert J Lefkowitz, 2012 Nobel Laureates in Chemistry. Proc Natl Acad Sci U S A 2013; 110: 5274–5275.
  • Joost P, Methner A. Phylogenetic analysis of 277 human G-protein-coupled receptors as a tool for the prediction of orphan receptor ligands. Genome Biol 2002; 3: 0063.1–0063.16.
  • Shinichi I, Tadahiro N, Toshiyasu S. The RAIG family member GPRC5D is associated with hard-keratinized structures. J Invest Dermatol 2004; 122: 565–573.
  • Kochhar DM, Jiang H, Penner JD, Johnson AT, Chandraratna RA. The use of a retinoid receptor antagonist in a new model to study vitamin A-dependent developmental events. Int J Dev Biol 1998; 42: 601–608.
  • Maden M. Retinoic acid in the development regeneration and maintenance of the nervous system. Nat Rev Neurosci 2007; 8: 755–765.
  • Bazzano G, Terezakis N, Attia H, Bazzano A, Dover R, Fenton D, Mandir N, Celleno L, Tamburro M, Jaconi S. Effect of retinoids on follicular cells. J Invest Dermatol 1993; 101: 138S–142S.
  • Fu HH, Xin PY, Xu YL, Liu Y, Wei YJ, Dong J, Cao YL, Zhou J. Bioinformatics analysis of UFGT gene from several economic plants. Genomics and Applied Biology 2010; 30: 92–102 (article in Chinese with English abstract).
  • Jin M, Wang L, Li S, Xing MX, Zhang X. Characterization and expression analysis of KAP7.1 KAP8.2 gene in Liaoning new- breeding cashmere goat hair follicle. Mol Biol Rep 2011; 38: 3023–3028.
  • Philpott MP, Green MR, Kealey T. Human hair growth in vitro. J Cell Sci 1990; 97: 463–471.
  • Zhou H, Chen Z, Zhang W, Xing G. Middle ear squamous papilloma: a report of four cases analyzed by HPV and EBV in situ hybridization. Oncol Lett 2014; 7: 41–46.
  • Jin M, Bai X, Zhang W, Gao WB, Wang W. Study on sequence variation of mitochondrial cytochrome b gene and phylogenetic relationships of two kinds of cashmere goats. Scientia Agricultura Sinica 2006; 39: 1897–1901.
  • Jin M, Hu JH. The breeding situation and development direction of Liaoning cashmere goat. Chinese Journal of Animal Science 2005; 41: 54–56.
  • Zhao M, Chen H, Wang X, Yu H, Wang M, Wang J, Lan XY, Zhang CF, Zhang LZ, Guo YK et al. aPCR-SSCP and DNA sequencing detecting two silent SNPs at KAP8.1 gene in the cashmere goat. Mol Biol Rep 2009; 36: 1387–1391.
  • Vassilatis DK, Hohmann JG, Zeng H, Li F, Ranchalis JE, Mortrud MT, Brown A, Rodriguez SS, Weller JR, Wright AC et al. The G protein-coupled receptor repertoires of human and mouse. Proc Natl Acad Sci U S A 2003; 100: 4903–4908.
  • Fredriksson R, Lagerström MC, Lundin LG, Schiöth HB. The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints. Mol Pharmacol 2003; 63: 1256–1272.
  • Lagerström MC, Hellström AR, Gloriam DE, Larsson TP, Schiöth HB, Fredriksson R. The G protein–coupled receptor subset of the chicken genome. PLoS Comput Biol 2006; 2: e54.
  • Ji YP, Zhen Z, Hu YH. The repertoire of G-protein-coupled receptors in Xenopus tropicalis. BMC Genomics 2009; 10: 263.
  • Bräuner-Osborne H,  Jensen AA,  Sheppard PO, Brodin B, Krogsgaard-Larsen P, O’Hara P. Cloning and characterization of a human orphan family C G-protein coupled receptor GPRC5D. Biochim Biophys Acta 2001; 1518: 237–248.
  • Harada Y, Yokota C, Habas R, Slusarski DC, He X. Retinoic acid-inducible G protein-coupled receptors bind to frizzled receptors and may activate non-canonical Wnt signaling. Biochem Biophys Res Commun 2007; 358: 968–975.
  • Raghu P, Rao M, Ramanathan S. Cross genome phylogenetic analysis of human and Drosophila G protein-coupled receptors: application to functional annotation of orphan receptors. BMC Genomics 2005; 6: 106.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Effect of gamma irradiation on inactivation of Ichthyophthirius multifiliis trophonts and its efficacy on host response in experimentally immunized rainbow trout (Oncorhynchus mykiss)

Marzieh HEIDARIEH, Maryam Hedayati RAD, Ali Reza MIRVAGHEFI, Adama DIALLO, Shalaleh MOUSAVI, Najmeh SHEIKHZADEH, Amir Ali SHAHBAZFAR

Response of 2 breeds of broiler chicks to experimental infection with low dose of Eimeria tenella sporulated oocysts

İsa Danladi JATAU, Augustine Nnamdi ODIKA, Mathew THLAMA

Meat quality characteristics in Kıvırcık lambs

Engin YARALI, Onur YILMAZ, İbrahim CEMAL, Orhan KARACA, Turgay TAŞKIN

Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds

Onur YILMAZ, Tamer SEZENLER, Nezih ATA, Yalçın YAMAN, İbrahim CEMAL, Orhan KARACA

Correlations among oocyte quality, hepatic triacylglycerols, and some blood metabolites in Carora breed cows during early postpartum

Leandro RODRIGUEZ, Oswaldo ROSENDO, Carlos PARRAGA, Armando OROPEZA

Calving assistance influences the occurrence of umbilical cord pathologies treated surgically in calves

Carlos MOSCUZZA, Horacio MILICICH, Guadalupe ALVAREZ, Betiana GUTIERREZ, Mariano NAHUM

Effect of gamma irradiation on inactivation of Ichthyophthirius multifliis trophonts and its efcacy on host response in experimentally immunized rainbow trout (Oncorhynchus mykiss)

Ali Reza MIRVAGHEFI, Najmeh SHEIKHZADEH, Amir Ali SHAHBAZFAR, Adama DIALLO, Marzieh HEIDARIEH, Maryam I RAD HEDAYAT, Shalaleh MOUSAVI

Proximate composition and mineral and fatty acid profiles of male and female jinga shrimps (Metapenaeus affinis, H. Milne Edwards, 1837)

Mehmet Tolga DİNÇER, İlker AYDIN

The effects of LH on progesterone production by cell subpopulations isolated from early and late luteal phase goat corpora lutea

Hakan KALENDER, Şevket ARIKAN, Özkan ŞİMŞEK

A detailed evaluation of the skeletal elements of the skull in the grey heron (Ardea cinerea)

Şükrü Hakan ATALGIN, Emine Ümran BOZKURT BÜYÜKÇOPUR, İbrahim KÜRTÜL