Activity of 3b-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin

Three-b-hydroxysteroid dehydrogenase (3b-HSD) is the enzyme responsible for progesterone production. This study aimed to determine whether 3b-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + IGF-I (1 or 10 ng/mL) in a serum-free medium for another 96 h to predict degree of luteinisation. Before and after incubation, granulosa cells were stained for 3b-HSD activity. The high dose of IGF-I (10 ng/mL) increased (P < 0.05) progesterone secretion over 2.5-fold compared with FSH alone or the low dose of IGF-I (1 ng/mL) in cells preincubated with FCS. This was clearly reflected by darker 3b-HSD staining than in cells exposed to FSH and low dose IGF-I.

Anahtar Kelimeler:

3b-Hydroxysteroid dehydrogenase, bovine granulosa cells, progesterone, FSH, LH, IGF-I

Activity of 3β-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin

Keywords:

3b-Hydroxysteroid dehydrogenase, bovine granulosa cells, progesterone, FSH, LH, IGF-I,

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Niswender GD. Molecular control of luteal secretion of progesterone. Reproduction 2002; 123: 333–339.
Luo W, Wiltbank MC. Distinct regulation by steroids of messenger RNAs for FSHR and CYP19A1 in bovine granulosa cells. Biol Reprod 2006; 75: 217–225.
Arikan S, Yigit AA. Size distribution of steroidogenic and nonsteroidogenic ovine luteal cells throughout pregnancy. Anim Sci 2002; 75: 427–432.
Arikan S, Yigit AA. Effects of cholesterol and cAMP on progesterone production in cultured luteal cells isolated from pseudopregnant cat ovaries. Anim Reprod Sci 2009; 115: 238– 246.
Arikan S, Kalender H, Simsek O. Effects of cholesterol on progesterone production by goat luteal cell subpopulations at two different stages of the luteal phase. Reprod Domest Anim 2010; 45: e434–e439.
Rocha RM, Lima LF, Alves AM, Celestino JJ, Matos MH, Lima- Verded IB, Bernucie MP, Lopesa CAP, Báof SN, Campello CC et al. Interaction between melatonin and follicle-stimulating hormone promotes in vitro development of caprine preantral follicles. Domest Anim Endocrinol 2012; 44: 1–9.
Mihm M, Bleach EC. Endocrine regulation of ovarian antral follicle development in cattle. Anim Reprod Sci 2003; 78: 217–
Kafi M, Mesbah SF, Davoodian N, Kadivar A. Fine structures of the oocyte in relation to serum, follicular fluid steroid hormones and IGF-I in the ovulatory-sized follicles in one- humped camel (Camelus dromedarius). Avicenna J Med Biotechnol 2014; 6: 57–61.
Spicer LJ, Echternkamp SE. The ovarian insulin and insulin- like growth factor system with an emphasis on domestic animals. Domest Anim Endocrin 1995; 12: 223–245.
Hastie PM, Haresign W. Expression of mRNAs encoding insulin-like growth factor (IGF) ligands, IGF receptors and IGF binding proteins during follicular growth and atresia in the ovine ovary throughout the oestrous cycle. Anim Reprod Sci 2006; 92: 284–299.
Silva JR, Figueiredo JR, van den Hurk R. Involvement of growth hormone (GH) and insulin-like growth factor (IGF) system in ovarian folliculogenesis. Theriogenology 2009; 71: 1193–1208.
Gutierrez CG, Campbell BK, Webb R. Development of a long-term bovine granulosa cell culture system: induction and maintenance of estradiol production, response to follicle- stimulating hormone, and morphological characteristics. Biol Reprod 1997; 56: 608–616.
Zhao J, Taverne MA, van den Weijden GC, Bevers MM, van den Hurk R. Insulin-like growth factor-I (IGF-I) stimulates the development of cultured rat pre-antral follicles. Mol Reprod Dev 2001; 58: 287–296.
Shanmugam M, Pandita S, Palta P. Effects of FSH and LH on steroid production by buffalo (Bubalus bubalis) granulosa cells cultured in vitro under serum-free conditions. Reprod Domest Anim 2010; 45: 922–926.
Spicer LJ, Alpizar E, Echternkamp SE. Effects of insulin, insulin-like growth factor I, and gonadotropins on bovine granulosa cell proliferation, progesterone production, estradiol production, and (or) insulin-like growth factor I production in vitro. J Anim Sci 1993; 71: 1232–1241.
Gong JG, McBride D, Bramley TA, Webb R. Effects of recombinant bovine somatotropin, insulin-like growth factor-I and insulin on bovine granulosa cell steroidogenesis in vitro. J Endocrinol 1994; 143: 157–164.
Glister C, Tannetta DS, Groome NP, Knight PG. Interactions between follicle-stimulating hormone and growth factors in modulating secretion of steroids and inhibin-related peptides by nonluteinized bovine granulosa cells. Biol Reprod 2001; 65: 1020–1028.
Payne AH, Downing JR, Wong KL. Luteinizing hormone receptors and progesterone synthesis in two distinct populations of leydig cells. Endocrinology 1980; 106: 1424– 1429.
Bao B, Thomas MG, Griffith MK, Burghardt RC, Williams GL. Steroidogenic activity, insulin-like growth factor-I production, and proliferation of granulosa and theca cells obtained from dominant preovulatory and nonovulatory follicles during the bovine estrous cycle: effects of low-density and high-density lipoproteins. Biol Reprod 1995; 53: 1271–1279.
Forde N, Mihm M, Canty MJ, Zielak AE, Baker PJ, Park S, Lonergan P, Smith GW, Coussens PM, Ireland JJ, et al. Differential expression of signal transduction factors in ovarian follicle development: a functional role for betaglycan and FIBP in granulosa cells in cattle. Physiol Genomics 2008; 33: 193–204.
Schams D, Koll R, Li CH. Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture. Endocrinology 1988; 116: 97–100.
Langhout DJ, Spicer LJ, Geisert RD. Development of a culture system for bovine granulosa cells: effects of growth hormone, estradiol, and gonadotropins on cell proliferation, steroidogenesis, and protein synthesis. J Anim Sci 1991; 69: 3321–3334.
Jimenez-Krassel F, Ireland JJ. Development and validation of a short-term, serum-free culture system for bovine granulosa cells: evaluation of the effects of somatotropin and growth hormone-releasing factor on estradiol production. J Dairy Sci 2002; 85: 68–78.
Xu Z, Garverick HA, Smith GW, Smith MF, Hamilton SA, Youngquist RS. Expression of follicle-stimulating hormone and luteinizing hormone receptor messenger ribonucleic acids in bovine follicles during first follicular wave. Biol Reprod 1995; 53: 951–957.
Ginther OJ, Beg MA, Donadeu FX, Bergfelt DR. Mechanism of follicle deviation in monovular farm species. Anim Reprod Sci 2003; 78: 239–257.