Evaluation of ovsynch protocols for timed artificial insemination in water buffaloes in Bangladesh

A total of 65 water buffaloes (groups A, B, and C) at =>60 days postpartum with a body condition score (BCS) of =>2.5 were selected to evaluate ovsynch protocols for timed artificial insemination (TAI). The group A buffaloes (n = 25) were treated with a simple ovsynch protocol (GnRH - Day 7 - PGF2 alpha - Day 2 - GnRH -16 h - TAI). The group B buffaloes (n = 22) received PGF2 alpha treatment 12 days before the initiation of simple ovsynch (PGF2 alpha at Day -12 + simple ovsynch; modified ovsynch). The group C buffaloes (n = 18) were treated with a double ovsynch protocol (GnRH - Day 7 - PGF2 alpha - Day 3 - GnRH - Day 7 - GnRH - Day 7 - PGF2 alpha - 48 h - GnRH - 16 h - TAI). Milk P4 ELISA was used for tracking ovulation and conception rates. Ovulation rates were higher in buffaloes that received the double ovsynch treatment (group C; 83.3%) than those with simple ovsynch (group A; 72.0%; P < 0.05). The group C cows (44.4%) achieved a higher conception rate than the cows of groups A (28.0%) and B (36.4%) (P < 0.05) and multiparous buffaloes having BCS of =>3.5 responded better to the ovsynch treatments than the primiparous ones (P < 0.05). The double ovsynch protocol increases both ovulation and conception rates in comparison to the simple and modified ovsynch protocols and is more effective in multiparous cows than in primiparous ones.

Evaluation of ovsynch protocols for timed artificial insemination in water buffaloes in Bangladesh

A total of 65 water buffaloes (groups A, B, and C) at =>60 days postpartum with a body condition score (BCS) of =>2.5 were selected to evaluate ovsynch protocols for timed artificial insemination (TAI). The group A buffaloes (n = 25) were treated with a simple ovsynch protocol (GnRH - Day 7 - PGF2 alpha - Day 2 - GnRH -16 h - TAI). The group B buffaloes (n = 22) received PGF2 alpha treatment 12 days before the initiation of simple ovsynch (PGF2 alpha at Day -12 + simple ovsynch; modified ovsynch). The group C buffaloes (n = 18) were treated with a double ovsynch protocol (GnRH - Day 7 - PGF2 alpha - Day 3 - GnRH - Day 7 - GnRH - Day 7 - PGF2 alpha - 48 h - GnRH - 16 h - TAI). Milk P4 ELISA was used for tracking ovulation and conception rates. Ovulation rates were higher in buffaloes that received the double ovsynch treatment (group C; 83.3%) than those with simple ovsynch (group A; 72.0%; P < 0.05). The group C cows (44.4%) achieved a higher conception rate than the cows of groups A (28.0%) and B (36.4%) (P < 0.05) and multiparous buffaloes having BCS of =>3.5 responded better to the ovsynch treatments than the primiparous ones (P < 0.05). The double ovsynch protocol increases both ovulation and conception rates in comparison to the simple and modified ovsynch protocols and is more effective in multiparous cows than in primiparous ones.

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  • Abdalla EB. Improving the reproductive performance of Egyptian buffalo cows by changing the management system. Anim Reprod Sci 2003; 75: 1–8.
  • Ali A, Fahmy S. Ovarian dynamics and milk progesterone concentrations in cycling and non-cycling buffalo-cows (Bubalus bubalis) during Ovsynch program. Theriogenology 2007; 68: 23–28.
  • El-Wishy AB. The postpartum buffalo: II. Acyclicity and anestrous. Anim Reprod Sci 2007; 97: 216–236.
  • Ullah N, Anwar M, Rizwan S, Murtaza S. Blood plasma progesterone concentrations in two different veins and comparison of progesterone concentrations and rectal palpation findings to determine ovarian cyclicity in the Nili- Ravi buffalo (Bubalus bubalis). Pak Vet J 2006; 26: 118–120.
  • McDougall S, Macmillan KL, Williamson NB. Factors associated with prolonged period of post partum anoestrus in pasture-fed dairy cattle. In: Proceedings of the XX World Buiatrics Congress; 1998. pp. 657–662.
  • Hoque MN, Talukder AK, Kamal MM, Jha AK, Bari FY, Shamsuddin M. Ovulation synchronization in water buffaloes guided by milk progesterone assay. J Embry Trans 2011; 26: 105–109.
  • Paul V, Prakash BS. Efficacy of Ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah buffaloes. Theriogenology 2005; 64: 1049–1060.
  • Berber RC de A, Madureira EH, Baruselli PS. Comparison of two Ovsynch protocols (GnRH versus LH) for fixed timed insemination in buffalo (Bubalus bubalis). Theriogenology 2002; 57: 1421–1430.
  • Neglia G, Gasparrini B, Palo RD, Rosa CD, Zicarelli L, Campanile G. Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranean Buffalo cows. Theriogenology 2003; 60: 125–133.
  • De Rensis F, Ronci G, Guarneri P, Nguyen BX, Presicce GA, Huszenicza G, Scaramuzzi RJ. Conception rate after fixed time insemination following Ovsynch protocol with and without progesterone supplementation in cyclic and non- cyclic Mediterranean Italian buffaloes (Bubalus bubalis). Theriogenology 2005; 63: 1824–1831.
  • Presicce GA, Senatore EM, Bella A, De Santis G, Barile VL, De Mauro GJ, Terzano GM, Steccob R, Parmeggiani A. Ovarian follicular dynamics and hormonal profiles in heifer and mixed- parity Mediterranean Italian buffaloes (Bubalus bubalis) following an estrus synchronization protocol. Theriogenology 2004; 61: 1343–1355.
  • Moreira F, Orlandi C, Risco CA, Mattos R, Lopes F, Thatcher WW. Effects of presynchronization and bovine somatotropin on pregnancy rates to timed artificial insemination protocol in lactating dairy cows. J Dairy Sci 2001; 84: 1646–1659.
  • Cerri RLA, Rutigliano HM, Bruno RGS, Chebel RC, Santos JEP. Effect of artificial insemination (AI) protocol on fertilization and embryo quality in high-producing dairy cows. J Dairy Sci 2005; 88: 86 (abstract).
  • Souza AH, Ayres H, Ferreira RM, Wiltbank MC. A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology 2008; 70: 208–215.
  • Cordoba MC, Fricke PM. Evaluation of two hormonal protocols for synchronization of ovulation and timed artificial insemination in dairy cows managed in grazing-based dairies. J Dairy Sci 2001; 84: 2700–2708.
  • Khan AHMSI. Development of milk progesterone ELISA and its application at AI field services in cattle. PhD, Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, Bangladesh, 2008.
  • Shamsuddin M, Bhuiyan MMU, Chanda PK, Alam MGS, Galloway D. Radioimmunoassay of milk progesterone as a tool for fertility control in smallholder dairy farms. Trop Anim Health Prod 2006; 38: 85–92.
  • Baruselli PS. Control of follicular development applied to reproduction biotechnologies in buffalo. In: Proceedings of the I Congresso Nazionale sull’Allevamento del Bufalo. Eboli, Italy; 2001. pp. 128–146.
  • Bridges A, Lake S, Lemenager R, Claeys M. Timed Artificial Insemination in Beef Cows: What are the Options? West Lafayette, IN, USA: Purdue University Cooperative Extension Service; 2012.
  • Baruselli PS, Carvalho NAT, Henriquez CEP, Amaral R, Nichi M, Reichert RH. Use of progesterone associated to “Ovsynch” protocol for timed artificial insemination in buffalo (Bubalus bubalis). In: Proceedings of the II Congresso Nazionale sull’Allevamento del Bufalo. Rome, Italy; 2003. pp. 265–268.
  • Cunha AP, Guenther JN, Maroney MJ, Giordano JO, Nascimento AB, Bas S, Ayres H, Wiltbank MC. Recent advances in ovulation synchronization and superovulation in dairy cattle. J Dairy Sci 2008; 91: 246.
  • Antal T, Faluhelyi S, Szabo I, Janaky T, Toth I, Faredine I, Laszlo F. Study of the relationship between milk progesterone profiles measured at the time of insemination and conception rate in buffalo. Acta Vet Hunga 1987; 35: 391–395.
  • Ferreira MBD, Lopes BC, Andrade IC, Conceicao VJ. Escore corporal e anestro pós-parto em primíparas zebu. Revista Brasileira Reproducao Animal 1997; 114–116 (in Portuguese).
  • Baruselli PS, Carvalho NAT, Reichert RH, Henriquez CEP, Nichi M. Pre-synchronization with GnRH 7 days before Ovsynch protocol for timed insemination in buffalo. In: Buffalo Symposium of Americas Proceedings; 2002. pp. 414–417.
  • Derar R, Hussein HA, Fahmy S, El-Sherry TM, Megahed G. The effect of parity on the efficacy of an ovulation synchronization (Ovsynch) protocol in buffalo (Bubalus bubalis). Anim Reprod 2012; 9: 52–60.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Evaluation of ovsynch protocols for timed artifcial insemination in water bufaloes in Bangladesh

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Calving assistance influences the occurrence of umbilical cord pathologies treated surgically in calves

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Activity of 3β-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under diferent concentrations of serum, insulin-like growth factor I, and gonadotropin

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