Stanimir YOTOV*, İvan FASULKOV, Nasko VASSILEV

Effect of ejaculation frequency on spermatozoa survival in diluted semen from Pleven Blackhead rams

The effect of ejaculation frequency on spermatozoa survival in diluted Pleven Blackhead ram semen was investigated. Thirty-two ejaculates were examined. From each ram 4 ejaculates were collected at 10-min intervals in the morning and in the afternoon. Semen parameters, namely, color, density, transparency, volume, concentration, motility, and abnormal sperm, for each ejaculate were determined. The sperm motility was assessed from the moment of dilution up to 60 min at 10-min intervals, then again at 90 min and 120 min. The semen characteristics were determined by means of "Motic Image Plus" equipment and the data were processed statistically. Sperm volume and concentration in semen samples decreased gradually with increase in ejaculation frequency. The values were significantly lower for the 4th ejaculate compared to preceding ones (P < 0.05). The highest survival rate (P < 0.05) of sperm was found in the 2nd ejaculate, and this trend was preserved up to the second hour. Up to 50 min after dilution, the sperm motility did not change considerably, after which it was found to be reduced (P < 0.05). The frequency of ejaculation and the period of semen collection had an impact on sperm motility in extended and short-time stored semen from Pleven Blackhead rams. The spermatozoa in a 2nd ejaculate showed higher survival rates and could be recommended for additional processing.

Anahtar Kelimeler:

Key words: Ram, frequency of ejaculation, semen, motility

Effect of ejaculation frequency on spermatozoa survival in diluted semen from Pleven Blackhead rams

Keywords:

Key words: Ram, frequency of ejaculation, semen, motility,

PDF

___

Durrant, B.S.: Th e importance and potential of artifi cial insemination in CANDES (companion animals, non-domestic, endangered species. Th eriogenology, 2009; 71: 113-122.
Paulenz, H., Söderquist, L., Pérez-Pé, R., Berg, K.: Eff ect of diff erent extenders and storage temperatures on sperm viability of liquid ram semen. Th eriogenology, 2002; 57: 823-836.
Sabev, M., Nikolov, I., Ivanova-Kicheva, M., Stefanov, R., Chemshirova, T., Baycheva, E., Popova, M.: Cryopreservation of ram sperm from autochthonous breeds during a non-mating season. J. Centr. Europ. Agric., 2006; 4: 677-682.
Erochin, A.S., Derjazencev, V.I.: Use of sulphagone at insemination of ewes. Ovtsevodstvo, 1990; 4: 37-37. (article in Russian with abstract in English)
Evans, G., Maxwell, W.M.C.: Handling and examination of semen. In: Maxwell, W. M.C., Salomon’s Artifi cial Insemination of Sheep and Goats. Butterworths, Sidney, 1987; 93-93.
Kasymov, K.T.: A progressive method of storing semen. Ovtsevodstvo, 1984; 7: 17-18. (article in Russian with abstract in English).
Salamon, S., Maxwell, W.M.C.: Storage of ram semen. Anim. Reprod. Sci., 2000; 62: 77-111.
Lightfoot, R.J., Restall, B.J.: Eff ects of site of insemination, sperm motility and genital tract contraction on transport of spermatozoa in the ewe. J. Reprod. Fertil., 1971; 26: 1-13.
Mandiki, S., Derycke, G., Bister, J., Paquay, R.: Infl uence of season and age on sexual maturation parameters of Texel, Suff olk and Ile-de-France rams 1. Testicular size, semen quality and reproductive capacity. Small Rumin. Res., 1998; 28: 67-79.
Moore, B., Mayer, D., McKenzie, F.: Factors infl uencing motility and metabolism in ram semen. J. Anim. Sci., 1940; 210-215.
Salamon, S., Maxwell, W.M.C.: Frozen storage of ram semen II. Cause of low fertility aft er cervical insemination and methods of improvement. Anim. Reprod. Sci., 1995; 38: 1-36.
Philips, P., Lardy, H.: A yolk-buff er pabulum for preservation of bull sperm. J. Diary Sci., 1940; 23: 399-404.
Willett, E., Salisbury, G.: Th e eff ect of various diluters, cooling rate, temperature of storage, and some other factors, on the livability of spermatozoa in stored samples of bull semen. Cornell Univ. Agric. Exp. Sta. Mem., 1942; 249.
Maxwell, W.M.C., Salamon, S.: Liquid storage of ram semen: a review. Reprod. Fertil. Dev., 1993; 5: 613-38.
Bonev, G., Dimitrov, S., Georgieva, S.: Comparison between time of ram semen storage and fertility in sheep. J. Anim. Sci., 2006; 43: 48-50. (article in Bulgarian with an abstract in English).
Salamon, S., Maxwell, W.M.C.: Frozen storage of ram semen I. Processing, freezing, thawing and fertility aft er cervical insemination. Anim. Reprod. Sci., 1995; 37: 185-249.
Gündoğan, M.: Seasonal variation in serum testosterone, T3 and andrological parameters of two Turkish sheep breeds. Small Rumin. Res., 2007; 67: 312-316.
Ollero, M., Muińo-Blanko, T., López-Pérez, M., Cebrián-Pérez, J.: Viability of ram spermatozoa in relation to the abstinence period and successive ejaculations. Inter. J. Androl., 1996; 19: 287-292.
Pruneda, A., Pinart, E., Briz, M., Sancho, S., Garcia-Gil, N., Badia, E., Kadar, E., Bassols, J., Bussalleu, E., Yeste, M., Bonet, S.: Eff ects of a high semen-collection frequency on the quality of sperm from ejaculates and from six epididymal regions in boars. Th eriogenology 2005; 63: 2219-2232.
Kaya, A., Aksoy, M., Tekeli, T.: Infl uence of ejaculation frequency on sperm characteristics, ionic composition and enzymatic activity of seminal plasma in rams. Small Rumin. Res., 2002; 44: 153-158.
Nel-Th emaat, L., Harding, G., Chandler, J., Chenevert, J., Damiani, P., Fernandez, J., Humes, P., Pope, C., Godke, R.: Quality and freezing qualities of fi rst and second ejaculates collected from endangered Gulf Coast Native rams. Anim. Reprod. Sci., 2006; 95: 251-261.
Kistanova, E., Vasileva, D., Grigorov, B., Metodiev, N.: Th e morphological and biochemical characteristics of the consecutive ejaculates from Il-de-France rams at various ages. Biotech. Anim. Husb., 2007; 23: 301-310.
Tjankov, S., Dimitrov, I.: Bulgarian sheep bred. In: Tjankov, S. et al., Sheep and goat breeding. Silve Press, Stara Zagora, 2000; 100-110. (In Bulgarian)
Fourie, P., Schwalbach, L., Neser, F., Van der Westhuizen, C.: Scrotal, testicular and semen characteristics of young Dorper rams managed under intensive and extensive conditions. Small Rumin. Res., 2004; 54: 53-59.
Foote, R.H.: Factors infl uencing the quantity of semen harvested from bulls, rams, boars and stallions. J. Anim. Sci., 1978; 47 (Suppl. 2): 1-11.
Strzezek, J., Kordan, W., Glogowski, J., Wysocki, P., Borkowski, K.: Infl uence of semen-collection frequency on sperm quality in boars, with special reference to biochemical markers. Reprod. Dom. Anim., 1995; 30: 85-94.
Stellfl ug, J.N., Berardinelli, J.G.: Ram mating behavior aft er long-term selection for reproductive rate in Rambouillet ewes. J. Anim. Sci., 2002; 80: 2588-2593.
Marengo, S.: Maturing the sperm: Unique mechanisms for modifying integral proteins in the sperm plasma membrane. Anim. Reprod. Sci., 2008; 105: 52-63.
Pickett, B.W., Komarek, R.J.: Lipid and dry weight to bovine seminal plasma and spermatozoa from fi rst and second ejaculates. J. Dairy Sci., 1967; 50: 742-746.