Güven URAZ, Hikmet Mavuş BULDU, A. Nedret KOÇ

Aflatoxin M1 contamination in cow’s milk in Kayseri (Central Turkey)

Bu çalışmada, Türkiye’de Kayseri’nin köylerindeki inek sütlerindeki Afl atoksin M1 düzeyinin belirlenmesi amaçlanmıştır. Toplam 90 süt örneğinde AFM1 kontaminasyonu ELISA (Enzyme Linked Immunoassay) yöntemi kullanarak çalışıldı. AFM1 düzeyinin ortalama değeri 59,9 ng/L, % 95 konfi dende intervali 54,4-65,5 ng/L, ve standart sapması 26,6 ng/L olarak belirlendi. Avrupa Birliği ve Türkiye gıda kodeks göre 63 (% 70)’ün limit sınırlarının üzerinde olması istatiksel olarak önemli bulunmuştur (P < 0,05). Bununla beraber köylere göre AFM1 düzeyindeki farklılık istatiksel olarak önemli değildir (P > 0,05). Bu bölgedeki inek sütlerinde AFM1 düzeyinin yüksek olması, önemli bir halk sağlığı sorunu olarak düşünülmelidir. Daha fazla araştırmalarla yüksek ısı altında steril edilen (UHT) süt örneklerinde Afl atoksin M1 düzeyi belirlenmelidir.

Kayseri’deki inek sütlerinde aflatoxin M1 kontaminasyonu

Th e purpose of this study was to determine the levels of afl atoxin M1 (AFM1) in the milk of cows in the villages of Kayseri province, Turkey. Ninety milk samples were investigated for AFM1 contamination using the enzyme linked immunoassay (ELISA). Th e mean value of AFM1 levels was 59.9 ng/L, 95% confi dence interval was 54.4-65.5 ng/L, and standard deviation was 26.6 ng/L. For 63 samples (70%) the values were signifi cantly above the limit permitted by European Union criteria (EU) and the Turkish Food Codex (TFC) (P < 0.05). However, the diff erences in AFM1 levels between villages were not signifi cant (P > 0.05). In conclusion, since the level of AFM1 in the milk of cows was higher in Kayseri province, this must be seen as a signifi cant public health problem. Further research must be conducted on the levels of AFM1 in milk samples sterilized using ultra high temperature (UHT).

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1. Doyle, M.P., Beuchat, L.R., Montuille, T.J.: Mycotoxigenic Molds, Food Microbiology, Asm Press, USA. 1997.
2. Anonymous.: European community comments for the codex committee on food additives and contaminants. CL 1999/13-GEN-CX 0016 FAC-Agenda item 16a. 2000.
3. Dragacci, S., Grosso, F., Pfouwathel-Marchan, N., Fremy, J.M., Venant, A.: Profi ciency testing for the evaluation of the ability of European Union-National Reference laboratories to determine afl atoxin M1 in milk at levels corresponding to the new European Union legislation. Food. Addit. Contam., 2001;18: 405-415.
4. Goldblatt, L.A.: Afl atoxin and its control. Econ. Bot., 1968; 22: 51-62.
5. Taydaş, E., Aşkın, O.: Occurrence of afl atoxin in red pepper J. Food., 1995; 20: 3-8.
6. Fukal, I., Brezina, P. Determination of afl atoxin M1 level in milk in the production of baby and children’s food using immunoassay. Nahrung., 1991; 35: 745-748.
7. de Sylos, C.M., Rodriguez-Amaya, D.B., Carvalho, P.R.: Occurrence of afl atoxin M1 in milk and dairy products commercialized in Campinas, Food. Addit. Contam., 1996; 13: 169-172.
8. Rodríguez Velasco, M.L., Calonge Delso, M.M., Ordónez Escudero, D.: ELISA and HPLC determination of the occurrence of afl atoxin M(1) in raw cow’s milk. Food Addit. Contam., 2003; 20: 276-80.
9. Sun, Z., Lu, P., Gail, M.H. Pee, D., Zhang, Q., Ming, L., Wang, J., Wu, Y., Liu, G., Wu, Y., Zhu, Y.: Increased risk of hepatocellular carcinoma in male hepatitis B surface antigen carriers with chronic hepatitis who have detectable urinary ofl atoxin metabolite M1. Hepatology, 1999; 30: 379-383.
10. Srivasta, V.P., Bu-Abbas, A., Alaa-Basung, W., Al-Johar, W., Al-Muft i, S., Siddigui, M.K. Afl atoxin M1 contamination in commercial samples of milk and dairy products in Kuwait, Food. Add. Con., 2001; 18: 993-997.
11. Saad, A.M., Abdelgadir, A.M., Moss, M.O.: Exposure of infants to afl atoxin M1 from mother’s breast milk in Abu Ahobi. Food. Addit. Contam., 1995; 12: 255-261.
12. Galvano, F., Galofaro, V., de Angelis, A., Galvano, M., Bognanno, M., Galvano, G.: Survey of the occurrence of afl atoxin M1 in dairy products marketed in Italy. J. Food. Prot., 1996; 1: 738-41.
13. Martins, H.M.: Afl atoxin M1 in raw and ultra-high temperature-treated milk commercialized in Portugal. Food. Addit. Contam., 2000; 17: 871-874.
14. Galvano, F., Galofaro, V., Ritieni, A., Begnonno, M., De Angelis, A., Galvano, G.: Survey of the occurrence of afl atoxin M1 in dairy products marketed in Italy, Food. Addit. Contam., 2001; 18: 644-646.
15. Bakırcı, I.: A study on the occurrence of afl atoxin M1 in milk and milk products produced in Van province of Turkey. Food. Control., 2001; 12, 47-51.
16. Oruç, H.H., Sonal, S.: Determination of afl atoxin M1 levels in cheese and milk consumed in Bursa, Turkey., Vet. Hum. Toxicol., 2001; 43: 292-293.
17. Gürbay, A., Aydın, S., Girgin, G., Engin , A.B., Şahin G.: Assessment of afl otoxin M1 levels in milk in Ankara, Turkey. Food. Control., 2006; 17: 1-4.
18. Unusan, N.: Occurrence of afl atoxin M1 in UHT milk in Turkey. Food. Chem. Toxicol., 2006; 44: 1897-1900.
19. Liman, B.C., Şeybek, N.: Quantitative analysis of afl atoxin M1 in raw milk contaminated at low levels. Toxicol. Lett., 2001; 123: 42.
20. Tajkarimi, M., Shojaee Aliabadi, F., Salah Nejad, M., Pursoltani, H., Motallebi, A.A., Mahdavi, H.: Seasonal study of afl atoxin M1 contamination in milk in fi ve regions in Iran. Int. J. Food. Microbiol., 2007; 116: 346-9.