DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance

Öz The purpose of this experiment was to investigate the effects of Beltsville thawing solution BTS , Androhep, and Dulbecco?s modified Eagle medium + Fatal bovine serum D-F as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 109/60 mL, 1.5 × 109/60 mL, and 1.5 × 109/40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 109/40 mL with D-F, Androhep, and BTS, respectively. The effects of above diluents on sperm motility and sow reproductive performance were studied by postcervical insemination. The appropriate semen dose for postcervical insemination was 1.5 × 109/40 mL. After thawing of frozen semen, sperm motility of semen diluted with D-F was significantly higher than that of semen diluted with Androhep and BTS. There was no significant difference in pregnancy rates among sows via artificial insemination. The litter size of sows using D-F diluted semen was significantly higher than that of sows using Androhep and BTS diluted semen. In conclusion, D-F freezing diluent can improve sperm motility of frozen boar semen after thawing, prolong sperm survival time, and increase sow reproductive performance.

Kaynakça

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Kaynak Göster

Bibtex @ { tbtkveterinary724429, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2020}, volume = {44}, pages = {220 - 226}, doi = {10.3906/vet-1910-100}, title = {DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance}, key = {cite}, author = {Zheng, Peng and Wang, Zımıng and Lı, Xıaoyu and Huang, Fushuo and Ma, Mıngjun and Huang, He} }
APA Zheng, P , Wang, Z , Lı, X , Huang, F , Ma, M , Huang, H . (2020). DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance . Turkish Journal of Veterinary and Animal Sciences , 44 (2) , 220-226 . DOI: 10.3906/vet-1910-100
MLA Zheng, P , Wang, Z , Lı, X , Huang, F , Ma, M , Huang, H . "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance" . Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 220-226 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/53838/724429>
Chicago Zheng, P , Wang, Z , Lı, X , Huang, F , Ma, M , Huang, H . "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance". Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 220-226
RIS TY - JOUR T1 - DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance AU - Peng Zheng , Zımıng Wang , Xıaoyu Lı , Fushuo Huang , Mıngjun Ma , He Huang Y1 - 2020 PY - 2020 N1 - doi: 10.3906/vet-1910-100 DO - 10.3906/vet-1910-100 T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 220 EP - 226 VL - 44 IS - 2 SN - 1300-0128-1303-6181 M3 - doi: 10.3906/vet-1910-100 UR - https://doi.org/10.3906/vet-1910-100 Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance %A Peng Zheng , Zımıng Wang , Xıaoyu Lı , Fushuo Huang , Mıngjun Ma , He Huang %T DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance %D 2020 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 44 %N 2 %R doi: 10.3906/vet-1910-100 %U 10.3906/vet-1910-100
ISNAD Zheng, Peng , Wang, Zımıng , Lı, Xıaoyu , Huang, Fushuo , Ma, Mıngjun , Huang, He . "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance". Turkish Journal of Veterinary and Animal Sciences 44 / 2 (Mart 2020): 220-226 . https://doi.org/10.3906/vet-1910-100
AMA Zheng P , Wang Z , Lı X , Huang F , Ma M , Huang H . DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(2): 220-226.
Vancouver Zheng P , Wang Z , Lı X , Huang F , Ma M , Huang H . DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(2): 220-226.
IEEE P. Zheng , Z. Wang , X. Lı , F. Huang , M. Ma ve H. Huang , "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance", Turkish Journal of Veterinary and Animal Sciences, c. 44, sayı. 2, ss. 220-226, Mar. 2020, doi:10.3906/vet-1910-100

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