Peng ZHENG, Zımıng WANG, Xıaoyu LI, Fushuo HUANG, Mıngjun MA, He HUANG

DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance

Öz The purpose of this experiment was to investigate the effects of Beltsville thawing solution BTS , Androhep, and Dulbecco?s modified Eagle medium + Fatal bovine serum D-F as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 109/60 mL, 1.5 × 109/60 mL, and 1.5 × 109/40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 109/40 mL with D-F, Androhep, and BTS, respectively. The effects of above diluents on sperm motility and sow reproductive performance were studied by postcervical insemination. The appropriate semen dose for postcervical insemination was 1.5 × 109/40 mL. After thawing of frozen semen, sperm motility of semen diluted with D-F was significantly higher than that of semen diluted with Androhep and BTS. There was no significant difference in pregnancy rates among sows via artificial insemination. The litter size of sows using D-F diluted semen was significantly higher than that of sows using Androhep and BTS diluted semen. In conclusion, D-F freezing diluent can improve sperm motility of frozen boar semen after thawing, prolong sperm survival time, and increase sow reproductive performance.

Anahtar Kelimeler:

Sow, insemination, frozen semen, thawing, sperm motility

Tam Metin

Kaynakça

1. Roca J, Vazquez JM, Gil M A, Cuello C, Parrilla I et al. Challenges in pig artificial insemination. Reproduction in Domestic Animals 2006; 41 (2): 43-53.

2. Bortolozzo FP, Menegat MB, Mellagi AP, Bernardi ML, Wentz I. New artificial insemination technologies for swine. Reproduction in Domestic Animals 2015; 50 (2): 80-84.

3. Didion BA, Braunb GD, Dugganc MV. Field fertility of frozen boar semen: a retrospective report comprising over 2600 AI services spanning a four year period. Animal Reproduction Science 2013; 137: 189-196.

4. Knox RV. The fertility of frozen boar sperm when used for artificial insemination. Reproduction in Domestic Animals 2015; 50 (2): 90-97.

5. Bailey JL, Lessard C, Jacques J, Breque C, Dobrinski I et al. Cryopreservation of boar semen and its future importance to the industry. Theriogenology 2008; 70 (8): 1251-1259.

6. Knox R V. Artificial insemination in pigs today. Theriogenology 2016; 85 (1): 83-93.

7. Gonzalez-Pena D, Knox RV, Rodriguez-Zas SL. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: a simulation study. Theriogenology 2016; 85 (2): 335-344.

8. Schulze M, Bortfeldt R, Schäfer J, Jung M, Fuchs-Kittowski F. Effect of vibration emissions during shipping of artificial insemination doses on boar semen quality. Animal Reproduction Science 2018; 192: 328-334.

9. Abeydeera LR, Day BN. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biology of reproduction 1997; 57 (4): 729-734.

10. Martinez EA, Vazquez JM, Roca J, Cuello C, Gil MA et al. An update on reproduction technologies with potential shortterm application in pig production. Reproduction in Domestic Animals 2005; 40 (4): 300-309.

11. Martinez EA, Vazquez JM, Roca J, Lucas X, Gil MA et al. Successful non-surgical deep intrauterine insemination with small numbers of spermatozoa in sows. Reproduction 2001; 122 (2): 289-296.

12. Bolarin A, Roca J, Rodriguez-Martinez H, Hernandez M, Vazquez J M et al. Dissimilarities in sows’ ovarian status at the insemination time could explain differences in fertility between farms when frozen-thawed semen is used. Theriogenology 2006; 65 (3): 669-680.

13. Wongtawan T, Saravia F, Wallgren M, Caballero I, RodriquezMartinez H. Fertility after deep intra-uterine artificial insemination of concentrated low-volume boar semen doses. Theriogenology 2006; 65 (4): 773-787.

14. Roca J, Carvajal G, Lucas X, Va´zquez JM, Martınez EA. Fertility of weaned sows after deep intrauterine insemination with a reduced number of frozen-thawed spermatozoa. Theriogenology 2003; 60 (1): 77-87.

15. Bathgate R, Eriksson BM, Thomson PC, Maxwell WM, Evans G. Field fertility of frozen-thawed boar sperm at low doses using non-surgical, deep uterine insemination. Animal Reproduction Science 2008; 103: 323-335.

16. Waterhouse KE, Hofmo PO, Tverdal A, Miller JR. Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm. Reproduction 2006; 131 (5): 887-894.

17. Hernandez M, Roca J, Gil MA, Vázquez JM, Martínez EA. Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability. Theriogenology 2007; 67 (9): 1436-1445.

18. Eriksson BM, Rodriguez-Martinez H. Effect of freezing and thawing rates on the post-thaw viability of boar spermatozoa frozen in FlatPacks and Maxi-straws. Animal Reproduction Science 2000; 63: 205-220.

19. Eriksson BM, Petersson H, Rodriguez-Martinez H. Field fertility with exported boar semen frozen in the new FlatPack container. Theriogenology 2002; 58 (6): 1065-1079.

20. Okazaki T, Abe S, Yoshida S, Shimada M. Seminal plasma damages sperm during cryopreservation, but its presence during thawing improves semen quality and conception rates in boars with poor postthaw semen quality. Theriogenology 2009; 71 (3): 491-498.

21. Grobfeld R, Sieg B, Struckmann C, Frenzel A, Maxwell WMC et al. New aspects of boar semen freezing strategies. Theriogenology 2008; 70 (8): 1225-1233.

22. Rozeboom KJ, Reicks DL, Wilson ME. The reproductive performance and factors affecting on-farm application of lowdose intrauterine deposit of semen in sows. Journal of Animal Science 2004; 82 (7): 2164-2168.

23. Karl W Spencer, Phil H Purdy, Harvey D Blackburn, Scott F Spiller, Terry S Stewart et al. Effect of number of motile, frozenthawed boar sperm and number of fixed-time inseminations on fertility in estrous-synchronized gilts. Animal Reproduction Science 2010; 121: 259-266.

24. Martinez EA, Vazquez JM, Roca J, Lucas X, Gil MA et al. Minimum number of spermatozoa required for normal fertility after deep intrauterine insemination in non-sedated sows. Reproduction 2002; 123 (1): 163-170.

25. Watson PF, Behan JR. Intrauterine insemination of sows with reduced sperm numbers: results of a commercially based field trial. Theriogenology 2002; 57 (6): 1683-1693.

26. Roberts PK, Bilkei G. Field experiences on post-cervical artificial insemination in the sows. Reproduction in Domestic Animals 2005; 40 (5): 489-491.

27. Waberski D, Weitze KF, Gleumes T, Schwarz M, Willmen T et al. Effect of time of insemination relative to ovulation on fertility with liquid and frozen boar semen. Theriogenology 1994; 42 (5): 831-840.

28. Spencer KW, Purdy PH, Blackburn HD, Spiller SF, Stewart TS et al. Effect of number of motile frozen-thawed boar sperm and number of fixed-time inseminations on fertility in estroussynchronized gilts. Animal Reproduction Science 2010; 121: 259-266.

29. Peng Z, Fushuo H, Mingjun M, Yaguan T, He H. Progesterone and pregnant mare serum gonadotropin improve the reproductive capacity of replacement gilts in non-estrus. Animal Production Science 2019; 59 (12): 2184-2190.

30. Adegoke EO, Xue W, Machebe NS, Adeniran SO, Hao W et al. Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MCLR) via TLR4/NF-kB and mitochondrial signaling pathways blockage. Ecotoxicology and Environmental Safety 2018; 166: 165-175.

31. Yamaguchi S, Funahashi H, Murakami T. Improved fertility in gilts and sows after artificial insemination of frozen-thawed boar semen by supplementation of semen extender with caffeine and CaCl2. Journal of Reproduction and Development 2009; 55 (6): 645-649.

32. Lopez Rodriguez A, Rijsselaere T, Vyt P, Van Soom A, Maes D. Effect of dilution temperature on boar semen quality. Reproduction in Domestic Animals 2012; 47 (5): 63-66.

33. Menegat MB, Mellagi AP, Bortolin RC, Menezes TA, Vargas AR et al. Sperm quality and oxidative status as affected by homogenization of liquid-stored boar semen diluted in shortand long-term extenders. Animal Reproduction Science 2017; 179: 67-79.

34. Riesenbeck A, Schulze M, Rüdiger K, Henning H, Waberski D. Quality control of boar sperm processing: implications from European AI centers and two spermatology reference laboratories. Reproduction in Domestic Animals 2015; 50 (2): 1-4.

Kaynak Göster

Bibtex	@ { tbtkveterinary724429, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2020}, volume = {44}, pages = {220 - 226}, doi = {10.3906/vet-1910-100}, title = {DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance}, key = {cite}, author = {Zheng, Peng and Wang, Zımıng and Lı, Xıaoyu and Huang, Fushuo and Ma, Mıngjun and Huang, He} }
APA	Zheng, P , Wang, Z , Lı, X , Huang, F , Ma, M , Huang, H . (2020). DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance . Turkish Journal of Veterinary and Animal Sciences , 44 (2) , 220-226 . DOI: 10.3906/vet-1910-100
MLA	Zheng, P , Wang, Z , Lı, X , Huang, F , Ma, M , Huang, H . "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance" . Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 220-226 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/53838/724429>
Chicago	Zheng, P , Wang, Z , Lı, X , Huang, F , Ma, M , Huang, H . "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance". Turkish Journal of Veterinary and Animal Sciences 44 (2020 ): 220-226
RIS	TY - JOUR T1 - DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance AU - Peng Zheng , Zımıng Wang , Xıaoyu Lı , Fushuo Huang , Mıngjun Ma , He Huang Y1 - 2020 PY - 2020 N1 - doi: 10.3906/vet-1910-100 DO - 10.3906/vet-1910-100 T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 220 EP - 226 VL - 44 IS - 2 SN - 1300-0128-1303-6181 M3 - doi: 10.3906/vet-1910-100 UR - https://doi.org/10.3906/vet-1910-100 Y2 - 2021 ER -
EndNote	%0 Turkish Journal of Veterinary and Animal Sciences DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance %A Peng Zheng , Zımıng Wang , Xıaoyu Lı , Fushuo Huang , Mıngjun Ma , He Huang %T DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance %D 2020 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 44 %N 2 %R doi: 10.3906/vet-1910-100 %U 10.3906/vet-1910-100
ISNAD	Zheng, Peng , Wang, Zımıng , Lı, Xıaoyu , Huang, Fushuo , Ma, Mıngjun , Huang, He . "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance". Turkish Journal of Veterinary and Animal Sciences 44 / 2 (Mart 2020): 220-226 . https://doi.org/10.3906/vet-1910-100
AMA	Zheng P , Wang Z , Lı X , Huang F , Ma M , Huang H . DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(2): 220-226.
Vancouver	Zheng P , Wang Z , Lı X , Huang F , Ma M , Huang H . DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance. Turkish Journal of Veterinary and Animal Sciences. 2020; 44(2): 220-226.
IEEE	P. Zheng , Z. Wang , X. Lı , F. Huang , M. Ma ve H. Huang , "DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance", Turkish Journal of Veterinary and Animal Sciences, c. 44, sayı. 2, ss. 220-226, Mar. 2020, doi:10.3906/vet-1910-100