Evangelos ZOIDIS, Athanasıos C. PAPPAS, Alı AL-WAELI, Constantınos A. GEORGIOU, George P. DANEZIS, Nıkos DEMIRIS, George ZERVAS, Konstantınos FEGEROS

Effects of selenium and zinc supplementation on cadmium toxicity in broilers

Cadmium Cd is a toxic metal regarded as an environmental pollutant. The potential ameliorative effect of simultaneous addition of Se and Zn to broilers against Cd toxicity was examined. A total of 180 as-hatched broilers were used. There were 4 replicate pens of 3 dietary treatments: T1, T2, and T3. In T1, Se, Cd, and Zn were added to a basal diet at 0.3, 0, and 100 ppm, in T2 at 0.3, 50, and 150 ppm, and in T3 at 0.5, 50, and 150 ppm respectively. Selenium, Cd, Ca, Co, Cu, Fe, Li, Mg, Mn, Sb, As, Cr, Pb, Mo, Ni, V, and Zn were determined in breast samples using ICP-MS. Simultaneous addition of Se and Zn to broilers partly ameliorated the negative effects of Cd. The concentrations of Cd, As, and V in breast were significantly affected by the treatments while the concentration of other examined elements remained unaffected.

Keywords:

Broilers, cadmium, ICP-MS, selenium, toxicity, zinc,

PDF

___

1. EFSA (European Food Safety Authority). Cadmium in food-scientific opinion of the panel on contaminants in the food chain. EFSA Journal 2009; 980: 1-139. doi: 10.2903/j. efsa.2009.980
2. Martelli A, Rousselet E, Dycke C, Bouron A, Moulis JM. Cadmium toxicity in animal cells by interference with essential metals. Biochimie 2006; 88 (11): 1807-1814. doi: 10.1016/j. biochi.2006.05.013
3. Cai K, Yu Y, Zhang M, Kim K. Concentration, source, and total health risks of cadmium in multiple media in densely populated areas, China. International Journal of Environmental Research and Public Health 2019; 16 (13): E2269. doi: 10.3390/ ijerph16132269
4. Figueroa BE. Are more restrictive food cadmium standards justifiable health safety measures or opportunistic barriers to trade? An answer from economics and public health. Science of the Total Environment 2010; 389 (1): 1-9. doi: 10.1016/j. scitotenv.2007.08.015
5. Klaassen CD, Liu J, Diwan BA. Metallothionein protection of cadmium toxicity. Toxicology and Applied Pharmacology 2009; 238 (3): 215-220. doi: 10.1016/j.taap.2009.03.026
6. McCarty MF. Zinc and multi-mineral supplementation should mitigate the pathogenic impact of cadmium exposure. Medical Hypotheses 2012; 79 (5): 642-648. doi: 10.1016/j. mehy.2012.07.043
7. Zhang R, Wang Y, Wang C, Zhao P, Liu H et al. Ameliorative effects of dietary selenium against cadmium toxicity is related to changes in trace elements in chicken kidneys. Biological Trace Element Research 2017; 176 (2): 391-400. doi: 10.1007/ s12011-016-0825-x
8. Branca JJV, Morucci G, Maresca M, Tenci B, Cascella R et al. Selenium and zinc: Two key players against cadmium-induced neuronal toxicity. Toxicology in Vitro 2018; 48: 159-169. doi: 10.1016/j.tiv.2018.01.007
9. El-Boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB. Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. Journal of Trace Elements in Medicine and Biology 2015; 29: 104-110. doi: 10.1016/j.jtemb.2014.05.009
10. Zoidis E, Seremelis I, Kontopoulos N, Danezis G. Seleniumdependent antioxidant enzymes: Actions and properties of selenoproteins. Antioxidants 2018; 7 (5): 66. doi: 10.3390/ antiox7050066
11. Jemai H, Messaoudi I, Chaouch A, Kerkeni A. Protective effect of zinc supplementation on blood antioxidant defense system in rats exposed to cadmium. Journal of Trace Elements in Medicine and Biology 2007; 21 (4): 269-273. doi: 10.1016/j. jtemb.2007.08.001
12. Jemai H, Lachkar AH, Messaoudi I, Kerkeni A. Effects of zinc pre-treatment on blood glutathione, serum zinc and kidney histological organisation in male rats exposed to cadmium. Journal of Trace Elements in Medicine and Biology 2010; 24 (4): 277-282. doi: 10.1016/j.jtemb.2010.07.001
13. Messaoudi I, Banni M, Saïd L, Saïd K, Kerkeni A. Involvement of selenoprotein P and GPx4 gene expression in cadmiuminduced testicular pathophysiology in rat. ChemicoBiological Interactions 2010; 188 (1): 94-101. doi: 10.1016/j. cbi.2010.07.012
14. Nolan TD, Brown D. The influence of elevated dietary zinc, selenium, and their combination on the suppressive effect of dietary and intraperitoneal cadmium on egg production in laying hens. Journal of Toxicology and Environmental Health, Part A 2000; 60 (8): 549-565. doi: 10.1080/00984100050082094
15. Nad P, Massanyi P, Skalicka M, Korenekova B, Cigankova V et al. The effect of cadmium in combination with zinc and selenium on ovarian structure in Japanese quails. Journal of Toxicology and Environmental Health, Part A 2007; 42 (13): 2017-2022. doi: 10.1080/10934520701629716
16. Al-Waeli A, Pappas AC, Zoidis E, Georgiou CA, Zervas G et al. The role of selenium in cadmium toxicity: Interactions with essential and toxic elements. British Poultry Science 2012; 53 (6): 817-827. doi: 10.1080/00071668.2012.751523
17. Al-Waeli A, Zoidis E, Pappas AC, Georgiou CA, Zervas G et al. The role of organic selenium in cadmium toxicity: Effects on broiler performance and health status. Animal 2013; 7 (3): 386-393. doi: 10.1017/S1751731112001590
18. Georgiou CA, Danezis GP. Elemental and isotopic mass spectrometry. In: Picó Y (editor). Advanced Mass Spectrometry for Food Safety and Quality. 1st ed. Amsterdam, Netherlands: Elsevier; 2015. pp. 131-243. doi: 10.1016/B978-0-444-63340- 8.00003-0
19. Klee G, D’Onofrio G, Van Assendelft OW, Bull B, Bunyaratvej A et al. Platelet counting by the RBC/platelet ratio method: a reference method. American Journal of Clinical Pathology 2001; 115 (3): 460-464. doi: 10.1309/w612-myep-fa7u-8uya
20. Li JL, Gao R, Li S, Wang JT, Tang ZX et al. Testicular toxicity induced by dietary cadmium in cocks and ameliorative effect by selenium. Biometals 2010; 23 (4): 695-705. doi: 10.1007/ s10534-010-9334-0
21. Jihen el H, Imed M, Fatima H, Abdelhamid K. Protective effects of selenium (Se) and zinc (Zn) on cadmium (Cd) toxicity in the liver and kidney of the rat: histology and Cd accumulation. Food and Chemical Toxicology 2008; 46 (11): 3522-3527. doi: 10.1016/j.fct.2008.08.037
22. Carpenter JW. Exotic Animal Formulary. 3rd ed. St Louis, MI, USA: Saunders; 2004.
23. Lazarus M, Orct T, Jurasoviae J, Blanuša M. The effect of dietary selenium supplementation on cadmium absorption and retention in suckling rats. Biometals 2009; 22 (6): 973-983. doi: 10.1007/s10534-009-9249-9
24. Pappas AC, Zoidis E, Georgiou CA, Demiris N, Surai PF et al. Influence of organic selenium supplementation on the accumulation of toxic and essential trace elements involved in the antioxidant system of chicken. Food Additives and Contaminants, Part A 2011; 28 (4): 446-454. doi: 10.1080/19440049.2010.549152
25. Feroci G, Badiello R, Fini, A. Interactions between different selenium compounds and zinc, cadmium and mercury. Journal of Trace Elements in Medicine and Biology 2005; 18 (3): 227- 234. doi: 10.1016/j.jtemb.2004.09.005
26. López Alonso M, Prieto Montaña F, Miranda M, Castillo C, Hernández J et al. Interactions between toxic (As, Cd, Hg and Pb) and nutritional essential (Ca, Co, Cr, Cu, Fe, Mn, Mo, Ni, Se, Zn) elements in the tissues of cattle from NW Spain. Biometals 2004; 17 (4): 389-397. doi: 10.1023/b:biom.0000029434.89679. a2
27. Nriagu J, Boughanen M, Linder A, Howe A, Grant C et al. Levels of As, Cd, Pb, Cu, Se and Zn in bovine kidneys and livers in Jamaica. Ecotoxicology and Environmental Safety 2009; 72 (2): 564-571. doi: 10.1016/j.ecoenv.2008.05.001
28. Sun HJ, Rathinasabapathi B, Wu B, Luo J, Pu LP et al. Arsenic and selenium toxicity and their interactive effects in humans. Environment International 2014; 69: 148-158. doi: 10.1016/j. envint.2014.04.019.
29. Kobayashi K, Himeno S, Satoh M, Kuroda J, Shibata N et al. Pentavalent vanadium induces hepatic metallothionein through interleukin-6-dependent and-independent mechanisms. Toxicology 2006; 228 (2-3): 162-170. doi: 10.1016/j.tox.2006.08.022