Zeki YILMAZ, Serhat TORUN, Annamaria PRATELLI

Distribution of antigentypes of canine parvovirus type 2 in dogs with hemorrhagec enteritis in Turkey

Bu çalışmada, hemorajik entreritisli 60 köpek dışkısından izole edilen canine parvovirus tip 2 (CPV2) izolatlarının antijenik karakterizasyonu ile ilgili sonuçları rapor edildi. Antijenik karakterizaşyon hemagglutination inhibition (Hl) testi ile CPV2'ye karşı dört monoklonal antikorda (mAbs) oluşan reaktiviteye göre değerlendirildi. CPV2, 60 köpeğin 21 'inde (% 35) tespit edildi ve antijenik tiplendirme yalnız >1:8 titreye sahip 16 köpekte gerçekleştirilebildi. Dokuz örneğin CPV2a, 7 örneğin de CPV2b olarak karakterize edilmesi; Türkiye'de Bursa bölgesinde yaşayan köpeklerde CPV2a varyantının CPV2b'den daha yaygın olduğunu göstermektedir.

Türkiye' de hemorajik enteritisli köpeklerde Canine parvovirus tip 2 nin antijenik tiplerinin dağılımı

The results of antigenic characterization of canine parvovirus type 2 (CPV2) isolated from the feces of 60 dogs with hemorrhagic enteritis are reported. Antigenic characterization was carried out by comparing the reactivity pattern of 4 monoclonal antibodies (mAbs) to CPV2 in the hemagglutination inhibition (HI) test. CPV2 was detected in 21 out of 60 dogs (35%) and antigenic typing was able to be performed for only 16 dogs with >1:8 titers. Nine samples were characterized as CPV2a and 7 as CPV2b, indicating that the CPV2a variant was more common than CPV2b in dog populations in Bursa province in Turkey.

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1. Buonavoglia, D., Cavalli, A., Pratelli, A., Martella, V., Greco, G., Tempesta, M., Buonavoglia, C: Antigenic analysis of canine parvovirus strains isolated in Italy. New Microbiol., 2000; 23: 93-96.
2. Battilani, M., Ciulli S., Tisato E., Prosperi, S.: Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy. Virus Res., 2002; 83: 149-157.
3. Buonavoglia, C, Martella V., Pratelli, A., Tempest, M., Cavalli, A., Buonavoglia, D., Bozzo, G., Elia, G., Decaro, N., Carmichael, L.: Evidence for evolution of canine parvovirus type 2 in Italy. J. Gen. Virol., 2001; 82: 3021-3025.
4. De Ybanez, R.R., Vela, C, Cortes, E., Simarro, I., Casal, J.I.: Identification of types of canine parvovirus circulating in Spain. Vet. Rec, 1995; 136: 174-175.
5. Martin, V., Najbar, W., Gueguen, S., Grousson, D., Eun, H.M., Lebreux, B., Aubert, A.: Treatment of canine parvoviral enteritis with interferon-omega in a placebo-controlled challenge trial. Vet. 10. Microbiol., 2002; 22: 115-127. 6. Greenwood, N.M., Chalmers, W.S.K., Baxendale, W., Thompson, H.: Comparison of isolates of canine parvovirus by monoclonal antibody and restriction enzyme-analysis. Vet. Rec, 1996; 138: 495-496.
7. Parrish, C.R., Aquadro, C.F., Strassheim, M.L., Evermann, J.F., Sgro, J.Y., Mohammed, H.O.: Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J. Virol., 1991; 65: 6544-6552.
8. Mochizuki, M., Hashimoto, M., Ishida, T.: Recent epidemiological status of canine viral enteric infections and Giardia infection in Japan. J. Vet. Med. Sci., 2001; 63: 573-575.
9. Pratelli, A., Cavalli, A., Normanno, G., De Palma, M.G., Pastorelli, G., Martella, V., Buonavoglia, C: Immunization of pups with maternally derived antibodies to canine parvovirus (CPV) using a modified-live variant (CPV-2b). J. Vet. Med. B Infect. Dis. Vet. Publ. Hlth. 2000; 47: 273-276.
10. Sagazio, P., Tempesta, M., Buonavoglia, D., Cirone, F., Buonavoglia, C: Antigenic characterization of canine parvovirus strains isolated in Italy. J. Virol. Meth., 1998; 73: 197-200.
11. Sakulwira, K., Vanopongtipagorn, P., Theamboonlers, A., Oraveerakul, K., Poorawan, Y.: Prevalence of canine coronavirus and parvovirus infections in dogs with gastroenteritis in Thailand. Vet. Med.-Czech., 2003; 48: 163-167.
12. Özkul, A., Keleş, İ., Karaoğlu, T., Çabalar, M., Burgu, L Detection and RFLP analysis of canine parvovirus (CPV) DNA by poiymerase chain reaction (PCR) in a dog. Turk. J. Vet. Anim. Sci., 2002; 26: 1201-1203. 13. Truyen, U., Steinel, A., Bruckner, L, Lutz, H., Mostl, K.: Distribution of antigen types of canine parvovirus in Switzerland, Austria and Germany. Schweiz. Arch. Tierheilkd., 2000; 142: 115-119.
14. Steinel, A., Venter, E.H., Van Vuuren, M., Parrish, C.R., Truyen, U.: Antigenic and genetic analysis of canine parvoviruses in southern Africa. J. Vet. Res., 1998; 65: 239-242.