Determination of the Seroprevalence of Leptospirosis in Cattle by MAT and ELISA in Hatay, Turkey

Five hundred and twelve serum samples collected from randomly selected cattle of different age and sex from the towns and central villages of Hatay during April-July 2003 were tested for antibodies against 3 different Leptospira interrogans serovars (grippotyphosa, hardjo and icterohaemorrhagie) using a microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA). Forty-five (8.8%) and 72 (14%) of the 512 serum samples tested were detected to be positive with MAT and ELISA, respectively. Of the 45 MAT positive serum samples, 26 (57.7%) were positive against serovar grippotyphosa, 9 (20%) were positive against serovar hardjo, 1 (0.2%) was positive against serovar icterohaemorrhagie and 9 (20%) were positive against serovar grippotyphosa + serovar hardjo. Of the 72 ELISA positive serum samples, 32 (44.4%) were positive against serovar grippotyphosa, 15 (20.8%) were positive against serovar hardjo, 2 (2.8%) were positive against serovar icterohaemorrhagie and 23 (31.9%) were positive against serovar grippotyphosa + serovar hardjo. The dominant serovar was grippotyphosa. Statistical analyses indicated a significant difference in seroprevalence among locations (P < 0.05); however, no significant differences in positivity rates were found with respect to the age or sex of the animals (P > 0.05).

Anahtar Kelimeler:

Leptospirosis, cattle, MAT, ELISA

Determination of the Seroprevalence of Leptospirosis in Cattle by MAT and ELISA in Hatay, Turkey

Keywords:

Leptospirosis, cattle, MAT, ELISA,

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