Metin ÖĞÜN, Nadide Nabil KAMİLOĞLU, Kutlay GÜRBULAK, Ebru BEYTUT

Effects of Vitamin A and beta-carotene injection on levels of vitamin E and on glutathione peroxidase activity inpregnant Tuj sheep

Bu araştırmada, çiftleşme öncesinde ve gebelik süresince koyunlara vitamin A ve β-karoten enjeksiyonu yapılmasının plazma vitamin E düzeyleri ile birlikte ertrosit glutatyon peraksidaz (GSH-Px) aktiviteleri üzerine etkilerinin belirlenmesi amaçlanmıştır. Bu amaçla sağlıklı 4-5 yaşlarında ve ortalama 57 kg ağırlığında 32 Tuj ırkı koyun ile 4 adet koç kullanıldı. Tüm koyunların östrusu sinkronize edildi. Bu amaçla medroksiprogesteron asetat (MPA) içeren süngerlerin uygulanmasını takiben 14 gün sonra, süngerler çıkarılarak her bir koyuna 500 IU PMSG intra muskular (i.m.) olarak enjekte edildi. PMSG enjeksiyonundan 24 saat sonra her gruba bir koç katılarak koyunlarla çiftleşitirildi. ilk grup kontrol grubu olarak değerlendirildi ve Freund's adjuvant incomplete i.m. enjekte edildi. 8 mg/kg β-karoten (II. Grup), 200,000 IU vitamin A (III. Grup) ve 100,000 IU vitamin A ve 4 mg/kg β-karoten (IV. Grup) kombinasyonu 30 gün aralıklarla çiftleşmeden 15 gün önce ve gebelik süresince i.m. olarak enjekte edildi. Plasma vitamin E düzeylerini ve eritrosit GSH-Px aktivitelerini belirlemek için kan numuneleri enjeksiyonların 1. ve 15. günleri ile doğumdan sonra V. jugularis'ten alındı. Bu çalışmada, enjeksiyonu takiben 2. ve 4. Grupların plazma vitamin E düzeylerinde gebelik süresince kontrol grubu ile karşılaştırıldığında istatistisel olarak önemli bir farklılık (P < 0,001) gözlendi. Aynı zamanda vitamin E düzeylerinin enjeksiyon zamanlarına bağlı olarak değiştiğini belirledik. Ayrıca plazma vitamin E düzeylerinin vitamin A enjeksiyonları ile çok az etkilendiğini saptadık. Eritrosit GSH-Px aktivitesi II., III., ve IV. Gruplarda kontrol grubuna göre yüksek olarak belirlendi (P < 0,001). Bu değerler β-karoten grubunda diğer gruplardan daha yüksek Sonuç olarak, vitamin A ve β-karoten enjeksiyonları ile vitamin E ve GSH-Px gibi antioksidanlarin desteklenmesi gebeliğe bağlı olarak oluşan serbest radikallerin istenmeyen etkilerini önleyebilir

Vitamin A ve Beta-Karoten enjeksiyonunun gebe Tuj koyunlarında vitamin E düzeyleri ve glutatyon peroksidaz aktivitesi üzerine etkileri

The objective of research was to study the effect of vitamin A and β-carotene injections, before breeding and during pregnancy, on the levels of vitamin E in plasma and on the activity of erythrocyte glutathione peroxidase (GSH-Px) in sheep. Thirty-two healthy female and four healthy male Tuj sheep, 4-5 years old and weighing approximately 57 kg each, were used. The female animals were divided into 4 groups with 8 sheep in each. The oestrus cycles of the ewes were synchronised by the application of sponges containing medroxyprogesterone acetate (MPA) and by the intramuscular administration of 500 IU of PMSG. Twenty-four hours after the injection of PMSG, a ram was added to each group and allowed to mate with the sheep. The first group was used as the control. 8 mg/kg of β-carotene (Group II), 200,000 IU of vitamin A (Group III) and a combination of 100,000 IU of vitamin A and 4 mg/kg of β-carotene (Group IV) were administered intramuscularly at 30-day intervals, 15 days before breeding and during pregnancy. To measure the levels of vitamin E in plasma and the activity of erythrocyte GSH-Px, blood samples were taken from the jugular vein on days 1 and 15 after injection and after the birth. A statistically significant difference (P < 0.001) was observed during pregnancy in the levels of vitamin E in the plasma of Groups II and IV following injection in comparison with those of the control group. It was also observed that vitamin E levels changed in the treated groups in relation to the time of injection. We further determined that the levels of vitamin E in plasma were little affected by injections of vitamin A. Erythrocyte GSH-Px activity increased in Groups II, III and IV compared with that of the control group (P < 0.001). This increase was higher in the β-carotene group than in the other groups. It was concluded that supporting aspects of the antioxidant system such as vitamin E levels and GSH-Px activity with vitamin A and β-carotene injections may prevent the undesired effects of the free radicals associated with pregnancy.

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