Özlem Orunç KILINÇ, Yaşar GÖZ, Nazmi YÜKSEK, Yildiray BAŞBUĞAN, Ali Bilgin YILMAZ, Ahmet Duran ATAŞ

Determination of serum cardiac biomarkers and plasma D-dimer levelsin anemic sheep with babesiosis

In this study, the cardiac effects of anemia and venous thromboembolism (VTE), reportedly caused by the hemolyzed erythrocytes occurring in sheep with babesiosis, were investigated using cardiac markers and D-dimer (DD). The study included 34 sheep: 24 infected Akkaraman sheep (1-3 years old, diagnosed with babesiosis based on clinical and laboratory findings) and a control group of 10 noninfected healthy sheep of the same breed and age. Hematocrit (Hct) levels were measured in blood obtained from both groups. Cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and aspartate aminotransferase (AST) levels were measured in serum samples. In addition, the levels of DD were also measured in plasma samples. Sheep with babesiosis were subsequently divided into 3 subgroups according to their Hct levels, which ranged from 13.2% to 16.3% in the first group (n = 8), 20.1% to 25.6% in the second group (n = 8), and 27.4% to 30.3% in the third group (n = 8). Evaluations of the measurements of cTnI, CK-MB, and AST levels indicated statistically significant differences between infected and healthy sheep. Statistically significant differences were not found for DD levels between the 2 groups.

Anahtar Kelimeler:

Babesia, cardiac biomarkers, D-dimer, hematocrit, sheep

Determination of serum cardiac biomarkers and plasma D-dimer levelsin anemic sheep with babesiosis

Keywords:

Babesia, cardiac biomarkers, D-dimer, hematocrit, sheep,

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Jacobson LS, Clark IA. The pathophysiology of canine
babesiosis: new approaches to an old puzzle. J S Afr Vet Assoc 1994; 65: 134–145.
Lobetti RG, Dvir E, Pearson J. Cardiac troponins in canine babesiosis. J Vet Intern Med 2002; 16: 63–68.
Fartashvand M, Nadalian MG, Sakha M, Safi S. Elevated serum cardiac troponin I in cattle with theileriosis. J Vet Intern Med 2013; 27: 194–199.
Diana A, Guglielmini C, Candini D, Pietra M, Cipone M. Cardiac arrhythmias associated with piroplasmosis in the horse: a case report. Vet J 2007; 174: 193–195.
Basbuğan Y, Ağaoğlu Z, Yüksek N. An investigation on serum troponin concentration in healthy ruminants. Kafkas Univ Vet Fak Derg 2010; 16: 641–645.
Ekici OD, Işık N. Investigation of the cardiotoxicity of imidocarb in lambs. Revue Med Vet 2011; 162: 40–44.
Cassar K, Bachoo P, Ford I, Greaves M, Brittenden J. Clopidogrel has no effect on D-dimer and thrombin-antithrombin III levels in patients with peripheral arterial disease undergoing peripheral percutaneous transluminal angioplasty. J Vasc Surg 2005; 42: 252–258.
Goehring LS, Soboll Hussey G, Gomez Diez M, Benedict K, Maxwell LK, Morley PS, Sloet van Oldruitenborgh-Oosterbaan MM, Lunn DP. Plasma D dimer concentrations during experimental EHV-1 infections of horses. J Vet Intern Med 2013; 27: 1535–1542.
Lykins JD, Ristic M, Weisiger RM, Huxsoll DL. Babesia microti: pathogenesis of parasite of human origin in the hamster. Exp Parasitol 1975; 37: 388–397.
Habela MA, Reina D, Navarrete I, Redondo E, Hernández S. Histopathological changes in sheep experimentally infected with Babesia ovis. Vet Parasitol 1991; 38: 1–12.
Soulsby EJL. Helminths, Arthropods and Protozoa of Domesticated Animals. 7th ed. London, UK: Bailliere Tindall; 1987.
Sevinç F, Dik B. Konya yöresindeki koyunlarda Babesia ovis’in ELISA ile teşhisi. Vet Bil Derg 1996; 12: 73–79 (in Turkish).
Yılmaz K, Özer E, Erkal N. Parazitli ve parazitsiz buzağılarda demir yetersizliği anemisi yönünden araştırmalar. FÜ Sağ Bil Ens Derg 1993; 7: 102–111 (in Turkish).
Ekici DO, Sevinc F, Isik N. Instability of ovine babesiosis in an endemic area in Turkey. Vet Parasitol 2012; 188: 372–375.
Çiçek H, Düzgün A, Emre Z, Karaer Z. Seroprevalence of Babesia ovis in sheep around Afyon. Turk J Vet Anim Sci 2004; 28: 683–686.
İnci A, İca A, Yıldırım A, Düzlü Ö. Identification of Babesia and Theileria species in small ruminants in Central Anatolia (Turkey) via reverse line blotting. Turk J Vet Anim Sci 2010; 34: 205–210.
Taşcı S. Van bölgesinde sığır ve koyunlarda görülen kene türleri ile bunların taşıdığı kan parazitleri arasındaki ilişkiler. Ankara Üniv Vet Fak Derg 1989; 36: 53–63 (in Turkish).
Tufan E, Aslan Ö. The determination of some blood parameters in sheep with naturally acute babesiosis. J Health Sci 2009; 18: 33–37.
Zulfigar S, Shahnawaz S, Ali M, Bhutta AM, Iqbal S, Hayat S, Qadir S, Latif M, Kiran N, Saeed A et al. Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab. Asian Pac J Trop Biomed 2012; 2: 104–108.
Karapinar T, Eroksuz Y, Beytut E, Sozdutmaz I, Eroksuz H, Dabak M. Increased plasma cardiac troponin I concentration in lambs with myocarditis. Vet Clin Pathol 2012; 41: 375–381.
Reyers F, Leisewitz AL, Lobetti RG, Milner RJ, Jacobson LS, van Zyl M. Canine babesiosis in South Africa—more than one disease. Does this serve as a model for falciparum malaria? Ann Trop Med Parasitol 1998; 92: 503–511.