Clinical and radiographic characterization of xenotransplantation of rat bonemarrow-derived mesenchymal stem cells for repair of radial defects of rabbit
This study aimed to evaluate the clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived mesenchymal stem cells (MSCs) for the repair of radial bone defects of rabbit. Bone marrow derived MSCs collected from Wistar rats were expanded, differentiated, and labeled in the laboratory. In the fourth passage, the cultures were pelleted in aliquots of 107 cells/50 μL and transplanted in situ into the lesions. Thirty-six rabbits were used in the in vivo study. In all animals, defects were created by removing a bone segment of 1.0 cm in length in the right radius. The rabbits were divided into four experimental groups: the control group (CG) received percutaneously administered 50 μL of phosphate buffer (PBS) in the radial bone defect region 24 h after lesion creation; group 1 (G1) had the radial bone defect filled with absorbable gelatin sponge soaked with 50 μL of PBS during the surgery; group 2 (G2) had the radial bone defect filled with absorbable gelatin sponge soaked with the MSC pellet; and group 3 (G3) received percutaneously the MSC pellet in the radial bone defect region 24 h after lesion creation. No clinical and radiographic changes consistent with cellular rejection were observed. Percutaneous MSC xenotransplantation contributed to the repair of experimental radial bone defects.
Clinical and radiographic characterization of xenotransplantation of rat bonemarrow-derived mesenchymal stem cells for repair of radial defects of rabbit
This study aimed to evaluate the clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived mesenchymal stem cells (MSCs) for the repair of radial bone defects of rabbit. Bone marrow derived MSCs collected from Wistar rats were expanded, differentiated, and labeled in the laboratory. In the fourth passage, the cultures were pelleted in aliquots of 107 cells/50 μL and transplanted in situ into the lesions. Thirty-six rabbits were used in the in vivo study. In all animals, defects were created by removing a bone segment of 1.0 cm in length in the right radius. The rabbits were divided into four experimental groups: the control group (CG) received percutaneously administered 50 μL of phosphate buffer (PBS) in the radial bone defect region 24 h after lesion creation; group 1 (G1) had the radial bone defect filled with absorbable gelatin sponge soaked with 50 μL of PBS during the surgery; group 2 (G2) had the radial bone defect filled with absorbable gelatin sponge soaked with the MSC pellet; and group 3 (G3) received percutaneously the MSC pellet in the radial bone defect region 24 h after lesion creation. No clinical and radiographic changes consistent with cellular rejection were observed. Percutaneous MSC xenotransplantation contributed to the repair of experimental radial bone defects.
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- Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284: 143–147.
- Caplan, AI. Why are MSCs therapeutic? New data: new insight. J Pathol 2009; 217: 318–324.
- Nauta AJ, Fibbe WE. Immunomodulatory properties of mesenchymal stroma cells. Blood 2007; 110: 3499–3506.
- Wan CD, Cheng R, Wang H, Liu T. Immunomodulatory effects of mesenchymal stem cells derived from adipose tissues in a rat orthotopic liver transplantation model. Hepatobiliary Pancreat Dis Int 2008; 7: 29–33.
- Patel SA, Sherman L, Munoz J, Rameshwar P. Immunological properties of mesenchymal stem cells and clinical implications. Arch Immunol Ther Exp 2008; 56: 1–8.
- Monteiro BS, Argôlo-Neto NM, Nardi NB, Chagastelles PC, Carvalho PH, Bonfá LP, Filgueiras RR, Reis AS, Del Carlo RJ. Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells. An Acad Bras Cienc 2012; 84: 841– 851.
- Bydlowski SP, Debes AA, Maselli LM, Janz FL. Características biológicas das células-tronco mesenquimais. Rev Bras Hematol Hemoter 2009; 31: 25–35 (in Portuguese).
- Ocarino NM, Boeloni JN, Goes AM, Silva JF, Marubayashi U, Serakides R. Osteogenic differentiation of mesenchymal stem cells from ostopenic rats subjected to physical activity with and without nitric oxide synthase inhibition. Nitric Oxide 2008; 19: 320–325.
- Kode JA, Mukherjee S, Joglekar MV, Hardikar AA. Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy 2009; 11: 377–339.
- Fischer UM, Harting MT, Jimenez F, Monzon-Posadas WO, Xue H, Savitz SI, Laine GA, Cox CS Jr. Pulmonary passage is a major obstacle for intravenous stem cell delivery: the pulmonary first-pass effect. Stem Cells Dev 2009; 18: 683–691.
- Zhao S, Wehner R, Bornhäuser M, Wassmuth R, Bachmann M, Schmitz M. Immunomodulatory properties of mesenchymal stromal cells and their therapeutic consequences for immune- mediated disorders. Stem Cells Dev 2010; 19: 607–614.
- Hernigou PH, Poignard A, Beaujean F, Rouard H. Percutaneous autologous bone marrow grafting for nonunions – influence of number and concentration of progenitor cells. J Bone Joint Surg Am 2005; 87: 430–437.
- Chuang CK, Wong TH, Hwang SM, Chang YH, Chen GY, Chiu YC, Huang SF, Hu YC. Baculovirus transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation. Mol Ther 2009; 17: 889–896.
- Chuang CK, Lin KJ, Lin CY, Chang YH, Yen TC, Hwang SM, Sung LY, Chen HC, Hu YC. Xenotransplantation of human mesenchymal stem cells into immunocompetent rats for calvarial bone repair. Tissue Eng Part A 2010; 16: 479–488.
- Shoji T, Li M, Mifune Y, Matsumoto T, Kawamoto A, Kwon SM, Kuroda T, Kuroda R, Kurosaka M, Asahara T. Local transplantation of human multipotent adipose-derived stem cells accelerates fracture healing via enhanced osteogenesis and angiogenesis. Lab Invest 2010; 90: 637–649.
- Kwan MD, Slater BJ, Wan DC, Longaker MT. Cell-based therapies for skeletal regenerative medicine. Hum Mol Genet 2008; 17: R93–R98.