Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

In this study, we compared 2 methods, liquid-phase blocking ELISA (LPBE) and solid-phase competition ELISA (SPCE), for the detection of antibodies to the structural proteins of foot-and-mouth disease virus (FMDV). These methods were compared using sera collected from cattle (n = 30) without a history of foot-and-mouth disease infection or vaccination, cattle (n = 180) vaccinated with oil-adjuvanted bivalent vaccine, and international reference sera (positive, weak positive, and negative) for FMDV serotypes O and A. The results showed that SPCE had a better specificity (96.67% for serotype O and 100% for serotype A) than LPBE (90% for serotype O and 93.33% for serotype A). Sensitivity of LPBE (97.22% for serotype O and 98.33% for serotype A) was almost equivalent to that of SPCE (98.33% for serotype O and 98.89% for serotype A). It can be concluded that SPCE is more suitable than LPBE for use as a screening test for the detection of antibodies against structural proteins of FMDV.

Anahtar Kelimeler:

Key words: Cattle, foot-and-mouth disease, structural protein, liquid-phase blocking ELISA, solid-phase competition ELISA

Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Keywords:

Key words: Cattle, foot-and-mouth disease, structural protein, liquid-phase blocking ELISA, solid-phase competition ELISA,

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Grubman MJ, Baxt B. Foot-and-mouth disease. Clin Microbiol Rev 2004; 17: 465–493.
Hammond JM, King D, Knowles N, Mioulet V, Li Y. Analysis of the Worldwide FMD Situation Trends and Regional Differences. In: FAO/OIE Global Conference on Foot and Mouth Disease Control. Bangkok, Thailand, 2012.
Alexandersen S, Mowat GN. Foot-and-mouth disease: host range and pathogenesis. Curr Top Microbiol Immunol 2005; 288: 9–42.
Acharya R, Fry E, Stuart D, Fox G, Rowlands D, Brown F. The three-dimensional structure of foot-and-mouth disease virus at 9 Ǻ resolution. Nature 1989; 337: 709–713.
Borrego B, Camarero JA, Mateu MG, Domingo E. A highly divergent antigenic site of foot-and-mouth disease virus retains its immunodominance. Viral Immunol 1995; 8: 11–18.
Domingo E, Escarmís C, Baranowski E, Ruiz-Jarabo CM, Carrillo E, Nşñez JI, Sobrino F. Evolution of foot-andmouth disease virus. Virus Res 2003; 91: 47–63.
Doel TR. FMD vaccines. Virus Res 2003; 91: 81–99.
McKercher PD, Graves JH. A review of the current status of oil adjuvants in foot-and-mouth disease vaccines. Dev Biol Standard 1977; 35: 107–112.
Wang G, Pan L, Zhang Y, Wang Y, Zhang Z, Lü J, Zhou P, Fang Y, Shoutian J. Intranasal delivery of cationic PLGA nano/ microparticles-loaded FMDV DNA vaccine encoding IL-6 elicited protective immunity against FMDV challenge. PLoS ONE 2011; 6: e27605.
Abu Elzein EME, Crowther JR. Detection and quantification of IgM, IgA, IgG 1 and IgG 2 antibodies against foot-andmouth disease virus from bovine sera using an enzyme-linked immunosorbent assay. J Hyg Camb 1981; 86: 79–85.
Smitsaart EN, Zanelli M, Rivera I, Fondevila N, Compaired D, Maradei E, Bianchi T, O’Donnell V, Schudel AA. Assessment using ELISA of the herd immunity levels induced in cattle by foot-and-mouth disease oil vaccines. Prev Vet Med 1998; 33: 283–296.
OIE. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 6th ed. Paris, France: OIE, 2012.
Mackay DKJ, Bulut AN, Rendle T, Davidson F, Ferris NP. A solid-phase competition ELISA for measuring antibody to footand-mouth disease virus. J Virol Methods 2001; 97: 33–48.
Hamblin C, Barnett ITR, Hedger RS. A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method ELISA. J Immunol Methods 1986; 93: 115–121.
Paiba GA, Anderson J, Paton DJ, Soldan AW, Alexandersen S, Corteyn M, Wilsden G, Hamblin P, MacKay DK, Donaldson AI. Validation of a foot-and-mouth disease antibody screening solid-phase competition ELISA (SPCE). J Virol Methods 2004; 115: 145–158.
Jacobson RH. Validation of serological assays for diagnosis of infectious diseases. Rev Sci Tech Off Int Epiz 1998; 17: 469– 4
OIE. Manual of Standards for Diagnostic Tests and Vaccines. 4th ed. Paris, France: OIE, 2000.
Klein J, Parlak U, Ozyoruk F, Christensen LS. The molecular epidemiology of foot-and-mouth disease virus serotypes A and O from 1998 to 2004 in Turkey. BMC Vet Res 2006; 4: 2–35.
Parlak Ü. Türkiye’de izole edilen şap viruslarinin genetik ve antijenik karakterizasyonu. PhD, Ankara University, Ankara, Turkey, 2005 (in Turkish).
Bulut N, Aktas, S. Turkey report to the EUFMD Commission, FMD in Turkey. In: Report of the 73rd Session of the EUFMD Executive Committee. Rome: FAO, 2006, pp. 23–27.
Sobrino F, Saiz M, Jimenez-Clavero MA, Nunez JI, Rosas MF, Baranowski E, Ley V. Foot-and-mouth disease virus: a long known virus, but a current threat. Vet Res 2001; 32: 1–30.
Clavijo A, Wright P, Kitching P. Developments in diagnostic techniques for differentiating infection from vaccination in foot-and-mouth disease. Vet J 2004; 167: 9–22.
Haas B. Application of the FMD liquid-phase blocking sandwich ELISA. Problems encountered in import/export serology and possible solutions. In: Session of the Research Group of the European Commission for the Control of Foot and Mouth Disease. Vienna, Austria: EuFMD, 1994, pp. 124– 1
Niedbalski W. Comparison of different ELISA methods for the detection of antibodies against foot-and-mouth disease virus (FMDV) type O. Bull Vet Inst Pulawy 2004; 48: 5–9.
Brocchi E, Grazioli S, Yadin H, Simone F. Validation of a solid phase competitive ELISA (SPBE) based on the use a single neutralising monoclonal antibody for the measurement of antibodies to FMDV type Asia 1. In: Session of the Research Group Standing Technical Committee of the European Commission for the Control of Foot and Mouth Disease. Crete, Greece: EuFMD, 2004, pp. 288–297.
Li Y, Swabey KG, Gibson D, Keel PJ, Hamblin P, Wilsden G, Corteyn M, Ferris NP. Evaluation of the solid phase competition ELISA for detecting antibodies against the six foot-and-mouth disease virus non-O serotypes. J Virol Methods 2012; 183: 125–131.
Martinez CS, Quintero M. The use of a liquid phase blocking ELISA kit for detection of antibodies against foot-and-mouth disease virus in Colombia. In: Proceedings of the Use of ELISA for Epidemiology and Control of Foot-and-Mouth Disease and Bovine Brucellosis in Latin America. Vienna, Austria, 1997.