Acute toxicity of diazinon and its effects on hematological parameters in the Indian carp, Cirrhinus mrigala (Hamilton)

Diazinon is a commonly used pesticide and its existence in the fresh water reservoirs of Pakistan has been reported by some authors. A semistatic experiment was conducted to find out the acute toxicity of diazinon as an aquatic pollutant on some hematological parameters of Indian carp (Cirrhinus mrigala). The 1, 24, 48, 72, and 96 h LC50 values of diazinon for Indian carp were estimated as 17.32 mg/L, 15.39 mg/L, 12.64 mg/L, 10.98 mg/L, and 8.15 mg/L respectively. A significant increase in the number of dead fish was observed as the concentration of diazinon and exposure time increased (P < 0.05). The results after acute exposure to 8.15 mg/L of diazinon for 96 h showed that compared to the control fish, the total erythrocytes, leukocytes, hemoglobin, and hematocrit values decreased significantly (P < 0.05) in exposed fish. The mean corpuscular volume and mean corpuscular hemoglobin increased significantly (P < 0.05), while the mean corpuscular hemoglobin concentration of both groups remained unchanged during the study. In conclusion, acute exposure to LC50 values of diazinon induced hematological alterations in Indian carp and offers a tool to evaluate toxicity derived alterations.

Acute toxicity of diazinon and its effects on hematological parameters in the Indian carp, Cirrhinus mrigala (Hamilton)

Diazinon is a commonly used pesticide and its existence in the fresh water reservoirs of Pakistan has been reported by some authors. A semistatic experiment was conducted to find out the acute toxicity of diazinon as an aquatic pollutant on some hematological parameters of Indian carp (Cirrhinus mrigala). The 1, 24, 48, 72, and 96 h LC50 values of diazinon for Indian carp were estimated as 17.32 mg/L, 15.39 mg/L, 12.64 mg/L, 10.98 mg/L, and 8.15 mg/L respectively. A significant increase in the number of dead fish was observed as the concentration of diazinon and exposure time increased (P < 0.05). The results after acute exposure to 8.15 mg/L of diazinon for 96 h showed that compared to the control fish, the total erythrocytes, leukocytes, hemoglobin, and hematocrit values decreased significantly (P < 0.05) in exposed fish. The mean corpuscular volume and mean corpuscular hemoglobin increased significantly (P < 0.05), while the mean corpuscular hemoglobin concentration of both groups remained unchanged during the study. In conclusion, acute exposure to LC50 values of diazinon induced hematological alterations in Indian carp and offers a tool to evaluate toxicity derived alterations.

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  • Abass A, Kudi AC, Moodi AJ. Spontaneous reactivation and aging kinetics of acetylcholinesterase inhibited by dichlorvos and diazinon. J Toxicol Sci 2011; 36: 237–241.
  • Oh HS, Lee SK, Kim YH, Roh JK. Mechanism of selective toxicity of diazinon to kill fish (Oryzias latipes) and loach (Misgurnus anguillicaudatus). Aqua Toxicol Risk Assess 1991; 14: 343–353.
  • Keizer J, DeAgostino G, Vittozzi I. The importance of biotransformation in the toxicity of xenobiotics to fish: toxicity and bioaccumulation of diazinon in guppy (Poecilia reticulata) and zebra fish (Brachydanio rerio). Aquat Toxicol 1991; 21: 239–254.
  • Khattak IU, Hafeez MA. Effect of malathion on blood parameters of the fish, Cyprinion watsoni. Pak J Zool 1996; 28: 45–
  • Singh NN, Srivastava AK. Formothion induced hematological changes in the freshwater Indian cat fish, Heteropneustes fossilis. J Ecotox Environ Monit 1994; 4: 137–140.
  • Benarji G, Rajendranath T. Hematological changes induced by an organophosphorus insecticide in a freshwater fish Clarias batrachus (L.). Trop Fresh Bio 1990; 2: 197–202.
  • Giron-Pirez MI, Santerre A, Gonzalez-Jaime F, Casas-Solis J, Hernandez-Coronado M, Peregrina-Sandoval J, Takemura A, Zaitseva G. Immunotoxicity and hepatic function evaluation in Nile tilapia (Oreochromis niloticus) exposed to diazinon. Fish Shellfish Immunol 2007; 23: 760–769.
  • Koprucu SS, Koprucu K, Ural MS, Ispir U, Pala M. Acute toxicity of organophosphorus pesticide diazinon and its effects on behaviour and some hematological parameters of fingerling European catfish (Silurus glanis). Pest Biochem Physiol 2006; 86: 99–105.
  • Ahmad Z. Acute toxicity and haematological changes in common carp (Cyprinus carpio) caused by diazinon exposure. Afr J Biotech 2011; 10: 13852–13859.
  • APHA. Standard Methods for the Examination of Water and Wastewater. 18th ed. Washington, DC, USA: American Public Health Association Publications; 1994.
  • Finney DJ. Probit Analysis. 3rd ed. New York, NY, USA: Cambridge University Press; 1971.
  • Svobodova Z, Pravda D, Palackova J. Unified Methods of Haematological Examination of Fish. 1st ed. Vodnany, Czechoslovakia: Research Institute of Fish Culture and Hydrobiology; 1991.
  • Giddings JM, Biever RC, Annunziato MF, Hosmer AJ. Effects of diazinon on large outdoor pond microcosms. Environ Toxicol Chem 1996; 15: 618–629.
  • Office of Pesticide Programs. Annual Report 2000. Washington, DC, USA: United States Environmental Protection Agency; 2000.
  • Shamoushaki MN, Soltani M, Kamali A, Imanpoor MR, Sharifpour I, Khara H. Effects of organophosphate, diazinon on some haematological and biochemical changes in Rutilus frisii kutum (Kamensky, 1901) male brood stocks. Iran J Fish Sci 2012; 1: 105–117.
  • Oruce EO, Usta D. Evaluation of oxidative stress responses and neurotoxicity potential of diazinon in different tissues of Cyprinus carpio. Environ Toxicol Pharmacol 2007; 23: 48–55.
  • Dutta HM, Meijer HJM. Sub-lethal effects of diazinon on the structure of the testis of bluegill, Lepomis macrochirus: a microscopic analysis. Environ Poll 2003; 125: 355–360.
  • Banaee M, Sureda A, Mirvaghefi AR, Ahmadi K. Effects of diazinon on biochemical parameters of blood n rainbow trout (Oncorhynchus mykiss). Pest Biochem Physiol 2011; 99: 1–6.
  • Uner N, Oruc EO, Sevgiler Y, Sahin N, Durmaz H, Usta D. Effects of diazinon on acetylcholinesterase activity and lipid peroxidation in the brain of Oreochromis niloticus. Environ Toxicol Pharmacol 2006; 21: 241–245.
  • Tavares DM, Martins ML, Nascimento KS. Evaluation of the hematological parameters in Piaractus mesopotamicus with Argulus sp. (Crustacea, Brachiura) infestation and treatment with organophosphate. Rev Bras Zool 1999; 16: 553–555.
  • Ramesh M, Saravanan M. Hematological and biochemical responses in freshwater fish Cyprinus carpio exposed to chlorpyrifos. Int J Integ Bio 2008; 3: 80–84.
  • Ali JHA, Rani VJ. Effect of phosalone on haematological indices in the tilapia, Oreochromis mossambicus. Turk J Vet Anim Sci 2009; 33: 407–411.
  • Svoboda M, Luskova V, Drastichova J, Zlabek V. The effect of diazinon on haematological indices of common carp (Cyprinus carpio). Acta Vet Brno 2001; 70: 457–465.
  • Adedeji OB, Adeyemo OK, Agbede SA. Effects of diazinon on blood parameters in the African catfish (Clarias gariepinus). Afr J Biotech 2009; 8: 3940–3946.
  • Rao DS. Carbaryl induced changes in the haematological, serum biochemical and immunological responses of common carp, Cyprinus carpio (L.) with special emphasis on herbal extracts as immunomodulators. PhD, Andhra University, Visakhapatnam, India, 2010.
  • Velisek J, Svobodova S, Piackova V. Effects of acute exposure to bifenthrin on some haematological, biochemical and histopathological parameters of rainbow trout (Oncorhynchus mykiss). Veter Medici 2009; 54: 131–137.
  • Khoshbavar-Rostami H, Soltani M, Yelghi S. Effects of diazinon on the hematological profiles of Acipenser stelletus and determination of LC50. J Agr Sci 2005; 12: 41–46.
  • Morgan DP, Stockdale EM, Roberts RJ, Walter HW. Anemia associated with exposure to lindane. Arch Environ Health 1980; 35: 307–310.
  • Nwani CD, Okeke OC, Onyishi G, Atama C, Chinekwu U, Eneje LO. Toxicity and effects of diazinon on behavior and some hematological parameters of African cat fish (Clarias gariepinus). Zool Ecol 2013; 23: 45–52.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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Acute toxicity of diazinon and its effects on hematological parameters in the Indian carp, Cirrhinus mrigala (Hamilton)

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