Xiaohong GUO, Yang LI, Mingxing CHU, Chunjing FENG, Ran DI, Qiuyue LIU, Tao FENG, Guiling CAO, Dongwei HUANG, Li FANG, Qianqian TANG, Yuehui MA, Kui LI

Polymorphism of 5’ regulatory region of caprine FSHR gene and its association with litter size in Jining Grey goat

Single nucleotide polymorphisms of the 5' regulatory region of the FSHR gene were detected in 3 goat breeds (Jining Grey, Boer, and Inner Mongolia Cashmere) by the polymerase chain reaction-single-strand conformation polymorphism method, and its effects on litter size in Jining Grey goats were also evaluated. Concerning primer P1, 2 genotypes (AA and BB) were detected in Boer goats, while only the AA genotype was identified in Jining Grey and Inner Mongolia Cashmere goats. Sequencing revealed a T->A transversion at the 26th position and an A->C transversion at the 61st position of the amplified region in genotype BB in comparison to genotype AA. Regarding primer P2, 3 genotypes (CC, CD, and DD) were identified in the 3 goat breeds mentioned above. Sequencing revealed a T->A transversion at the 70th position and a G->C transversion at the 130th position of the amplified region in genotype DD compared with CC. Does with genotype CC had 0.46 (P < 0.05) or 1.03 (P < 0.05) kids more than those with genotype CD or DD, respectively, while does with CD had 0.57 (P < 0.05) kids more than those with DD in Jining Grey goats. These results preliminarily showed that allele C of the FSHR gene was a potential marker for improving litter size in goats.

Polymorphism of 5’ regulatory region of caprine FSHR gene and its association with litter size in Jining Grey goat

Keywords:

Key words: Goat, litter size, FSHR gene, polymerase chain reaction-single-strand conformation polymorphism,

PDF

___

Dankbar, B., Brinkworth, M.H., Schlatt, S., Weinbauer, G.F., Nieschlag, E., Gromoll, J.: Ubiquitous expression of the androgen receptor and testis-specific expression of the FSH receptor in the cynomolgus monkey (Macaca fascicularis) revealed by a ribonuclease protection assay. J. Steroid Biochem. Mol. Biol., 1995; 55: 35–41.

Ranniki, A.S., Zhang, F.P., Huhtaniemi I.T.: Ontogeny of follicle-stimulating hormone receptor gene expression in the rat testis and ovary. Mol. Cell Endocrinol., 1995; 107: 199–208.

Dias, J.A., Cohen, B.D., Lindau-Shepard, B., Nechamen, C.A., Peterson, A.J., Schmidt, A.: Molecular, structural, and cellular biology of follitropin and follitropin receptor. Vitam. Horm., 2002; 64: 249–322.

Bogerd, J.: Ligand-selective determinants in gonadotropin receptors. Mol. Cell Endocrinol., 2007; 260–262: 144–152.

Gharib, S.D., Wierman, M.E., Shupnik, M.A., Chin, W.W.: Molecular biology of pituitary gonadotropins. Endocr. Rev., 1990; 11: 177–199.

Nieschlag, E., Simoni, M., Gromoll, J., Weinbauer, G.F.: Role of FSH in the regulation of spermatogenesis: clinical aspects. Clin. Endocrinol., 1999; 51: 139–146.

McNeilly, A.S., Souza, C.J., Baird, D.T., Swanston, I.A., McVerry, J., Crawford, J., Cranfield, M., Lincoln, G.A.: Production of inhibin A not B in rams: changes in plasma inhibin A during testis growth, and expression of inhibin/activin subunit mRNA and protein in adult testis. Reproduction, 2002; 123: 827–835.

George, J.W., Dille, E.A., Heckert, L.L.: Current concepts of follicle-stimulating hormone receptor gene regulation. Biol. Reprod., 2011; 84: 7–17.

Du, J., Zhang, W., Guo, L., Zhang, Z., Shi, H., Wang, J., Zhang, H., Gao, L., Feng, G., He, L.: Two FSHR variants, haplotypes and meta-analysis in Chinese women with premature ovarian failure and polycystic ovary syndrome. Mol. Genet. Metab., 2010; 100: 292–295.

Nakamura, Y., Maekawa, R., Yamagata, Y., Tamura, I., Sugino, N.: A novel mutation in exon 8 of the follicle-stimulating hormone receptor in a woman with primary amenorrhea. Gynecol. Endocrinol., 2008; 24: 708–712.

Achrekar, S.K., Modi, D.N., Meherji, P.K., Patel, Z.M., Mahale, S.D.: Follicle stimulating hormone receptor gene variants in women with primary and secondary amenorrhea. J. Assist. Reprod. Genet., 2010; 27: 317–326.

Pengo, M., Ferlin, A., Arredi, B., Ganz, F., Selice, R., Garolla, A., Foresta, C.: FSH receptor gene polymorphisms in fertile and infertile Italian men. Reprod. Biomed. Online, 2006; 13: 795–800.

Tu, Y.R.: The Sheep and Goat Breeds in China. Shanghai Science and Technology Press, Shanghai. 1989.

Malan, S.W.: The improved Boer goat. Small Rumin. Res., 2000; 36: 165–170.

Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA. 1989.

Song, M.L., Ma, X.M., Wang, J.M.: Single nucleotide polymorphism in goat FSHR gene and its association with superovulation. Acta Ecologiae Animalis Domastici, 2006; 27: 25–28 (article in Chinese with an English abstract).

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S.: MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 2011; 28: 2731–2739.

Marson, E.P., Ferraz, J.B., Meirelles, F.V., Balieiro, J.C., Eler, J.P.: Effects of polymorphisms of LHR and FSHR genes on sexual precocity in a Bos taurus × Bos indicus beef composite population. Genet. Mol. Res., 2008; 7: 243–251.

Simoni, M., Nieschlag, E., Gromoll, J.: Isoforms and single nucleotide polymorphisms of the FSH receptor gene: implications for human reproduction. Hum. Reprod. Update, 2002; 85: 413–421.

Wunsch, A., Sonntag, B., Simoni, M.: Polymorphism of the FSH receptor and ovarian response to FSH. Ann. Endocrinol., 2007; 68: 160–166.

Théron-Gérard, L., Pasquier, M., Czernichow, C., CédrinDurnerin, I., Hugues, J.N.: Follicle-stimulating hormone receptor polymorphism and ovarian function. Gynecol. Obstet. Fertil., 2007; 35: 135–141.

Mohiyiddeen, L., Nardo, L.G.: Single-nucleotide polymorphisms in the FSH receptor gene and ovarian performance: future role in IVF. Hum. Fertil. (Camb.), 2010; 13: 72–78.

Cui, H.X., Zhao, S.M., Cheng, M.L., Guo, L., Ye, R.Q., Liu, W.Q., Gao, S.Z.: Cloning and expression levels of genes relating to the ovulation rate of the Yunling black goat. Biol. Reprod., 2009; 80: 219–226.

Chu, M.X., Guo, X.H., Feng, C.J., Li, Y., Huang, D.W., Feng, T., Cao, G.L., Fang, L., Di, R., Tang, Q.Q., Ma, Y.H., Li, K.: Polymorphism of 5′ regulatory region of ovine FSHR gene and its association with litter size in Small Tail Han sheep. Mol. Biol. Rep., 2012; 39: 3721–3725.

Zhu, J., Yang, S.L., Ouyang, X.X., Liu, H.X., Hu, S.G.: Relationship between polymorphism of follicle stimulating hormone receptor gene and litter size traits in goats. Grassfeeding Livestock, 2007; 1: 12–15 (article in Chinese with an English abstract).