The effects of prenatal long-duration exposure to 900-MHz electromagnetic field on the 21-day-old newborn male rat liver
Background/aim: To determine what effect a 900-MHz electromagnetic field (EMF) applied in the prenatal period would have on the liver in the postnatal period. Materials and methods: At the start of the study, adult pregnant rats were divided into two groups, control and experimental. The experimental group was exposed to a 900-MHz EMF for 1 h daily during days 13?21 of pregnancy. After birth, no procedure was performed on either mothers or pups. Male rat pups (n = 6) from the control group mothers (CGMR) and male rat pups (n = 6) from the experimental group mothers (EGMR) were sacrificed on postnatal day 21. Results: Biochemical analyses showed that malondialdehyde and superoxide dismutase values increased and glutathione levels decreased in the EGMR pups. Marked hydropic degeneration in the parenchyma, particularly in pericentral regions, was observed in light microscopic examination of EGMR sections stained with hematoxylin and eosin. Examinations under transmission electron microscope revealed vacuolization in the mitochondria, expansion in the endoplasmic reticulum, and necrotic hepatocytes. Conclusion: The study results show that a 900-MHz EMF applied in the prenatal period caused oxidative stress and pathological alterations in the liver in the postnatal period.
The effects of prenatal long-duration exposure to 900-MHz electromagnetic field on the 21-day-old newborn male rat liver
Background/aim: To determine what effect a 900-MHz electromagnetic field (EMF) applied in the prenatal period would have on the liver in the postnatal period. Materials and methods: At the start of the study, adult pregnant rats were divided into two groups, control and experimental. The experimental group was exposed to a 900-MHz EMF for 1 h daily during days 13?21 of pregnancy. After birth, no procedure was performed on either mothers or pups. Male rat pups (n = 6) from the control group mothers (CGMR) and male rat pups (n = 6) from the experimental group mothers (EGMR) were sacrificed on postnatal day 21. Results: Biochemical analyses showed that malondialdehyde and superoxide dismutase values increased and glutathione levels decreased in the EGMR pups. Marked hydropic degeneration in the parenchyma, particularly in pericentral regions, was observed in light microscopic examination of EGMR sections stained with hematoxylin and eosin. Examinations under transmission electron microscope revealed vacuolization in the mitochondria, expansion in the endoplasmic reticulum, and necrotic hepatocytes. Conclusion: The study results show that a 900-MHz EMF applied in the prenatal period caused oxidative stress and pathological alterations in the liver in the postnatal period.
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- Ozguner F, Oktem F, Ayata A, Koyu A, Yilmaz HR. A novel antioxidant agent caffeic acid phenethyl ester prevents long- term mobile phone exposure-induced renal impairment in rat. Mol Cell Biochem 2005; 277: 73–80.
- Maaroufi K, Had-Aissouni L, Melon C, Sakly M, Abdelmelek H, Poucet B, Save E. Spatial learning, monoamines and oxidative stress in rats exposed to 900 MHz electromagnetic field in combination with iron overload. Behav Brain Res 2014; 258: 80–89.
- Aydin B, Akar A. Effects of a 900-MHz electromagnetic field on oxidative stress parameters in rat lymphoid organs, polymorphonuclear leukocytes and plasma. Arch Med Res 2011; 42: 261–267.
- Esmekaya MA, Aytekin E, Ozgur E, Güler G, Ergun MA, Omeroğlu S, Seyhan N. Mutagenic and morphologic impacts of 1.8 GHz radiofrequency radiation on human peripheral blood lymphocytes (hPBLs) and possible protective role of pre-treatment with Ginkgo biloba (EGb 761). Sci Total Environ 2011; 410–411: 59–64.
- Kiray A, Tayefi H, Kiray M, Bagriyanik HA, Pekcetin C, Ergur BU, Ozogul C. The effects of exposure to electromagnetic field on rat myocardium. Toxicol Ind Health 2013; 29: 418–425.
- Hekmat A, Saboury AA, Moosavi-Movahedi AA. The toxic effects of mobile phone radiofrequency (940 MHz) on the structure of calf thymus DNA. Ecotoxicol Environ Saf 2013; 88: 35–41.
- Deng Y, Zhang Y, Jia S, Liu J, Liu Y, Xu W, Liu L. Effects of aluminum and extremely low frequency electromagnetic radiation on oxidative stress and memory in brain of mice. Biol Trace Elem Res 2013; 156: 243–252.
- Ozturk A, Baltaci AK, Mogulkoc R, Oztekin E. Zinc prevention of electromagnetically induced damage to rat testicle and kidney tissues. Biol Trace Elem Res 2003; 96: 247–254.
- Salem A, Hafedh A, Rached A, Mohsen S, Khémais BR. Zinc prevents hematological and biochemical alterations induced by static magnetic field in rats. Pharmacol Rep 2005; 57: 616–622.
- Hancı H, Odacı E, Kaya H, Aliyazıcıoğlu Y, Turan İ, Demir S, Çolakoğlu S. The effect of prenatal exposure to 900-MHz electromagnetic field on the 21-old-day rat testicle. Reprod Toxicol 2013; 42: 203–209.
- İkinci A, Odacı E, Yıldırım M, Kaya H, Akça M, Hancı H, Aslan A, Sönmez OF, Baş O. The effects of prenatal exposure to a 900 megahertz electromagnetic field on hippocampus morphology and learning behavior in rat pups. NeuroQuantology 2013; 11: 582–590.
- Baş O, Sönmez OF, Aslan A, İkinci A, Hancı H, Yıldırım M, Kaya H, Akça M, Odacı E. Pyramidal cell loss in the cornu ammonis of 32-day-old female rats following exposure to a 900 megahertz electromagnetic field during prenatal days 13–21. NeuroQuantology 2013; 11: 591–599.
- Odacı E, İkinci A, Yıldırım M, Kaya H, Akça M, Hancı H, Sönmez OF, Aslan A, Okuyan M, Baş O. The effects of 900 megahertz electromagnetic field applied in the prenatal period on spinal cord morphology and motor behavior in female rat pups. NeuroQuantology 2013; 11: 573–581.
- Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497–500.
- Aebi H. Catalase. Medhods Enzymol 1984; 105: 121–126.
- Sedlak J, Lindsay RH. Estimation of total, protein-bound, and non- protein sulfhydryls groups in tissue with Ellman’s reagent. Anal Biochem 1968; 25: 192–205.
- Ohkawa H, Ohishi H, Yagi K. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351–358.
- Canan S, Aktaş A, Ulkay MB, Colakoglu S, Ragbetli MC, Ayyildiz M, Geuna S, Kaplan S. Prenatal exposure to a non- steroidal anti-inflammatory drug or saline solution impairs sciatic nerve morphology: a stereological and histological study. Int J Dev Neurosci 2008; 26: 733–738.
- Gokcimen A, Rağbetli MC, Baş O, Tunc AT, Aslan H, Yazici AC, Kaplan S. Effect of prenatal exposure to an anti-inflammatory drug on neuron number in cornu ammonis and dentate gyrus of the rat hippocampus: a stereological study. Brain Res 2007; 1127: 185–192.
- Sonmez OF, Odaci E, Bas O, Kaplan S. Purkinje cell number decreases in the adult female rat cerebellum following exposure to 900 MHz to electromagnetic field. Brain Res 2010; 1356: 95– 101.
- Cakir DU, Yokus B, Akdag MZ, Sert C, Mete N. Alterations of hematological variations in rats exposed to extremely low frequency magnetic fields (50 Hz). Arch Med Res 2009; 40: 352–356.
- Şekeroğlu V, Akar A, Atlı Şekeroğlu Z. Cytotoxic and genotoxic effects of high-frequency electromagnetic fields (GSM 1800 MHz) on immature and mature rats. Ecotoxicol Environ Saf 2012; 80: 140–144.
- Nisbet HO, Nisbet C, Akar A, Cevik M, Karayigit MO. Effects of exposure to electromagnetic field (1.8/0.9 GHz) on testicular function and structure in growing rats. Res Vet Sci 2012; 93: 1001–1005.
- Odaci E, Bas O, Kaplan S. Effects of prenatal exposure to a 900 MHz electromagnetic field on the dentate gyrus of rats: a stereological and histopathological study. Brain Res 2008; 1238: 224–229.
- Tenorio BM, Jimenez GC, Morais RN, Torres SM, Albuquerque Nogueira R, Silva Junior VA. Testicular development evaluation in rats exposed to 60 Hz and 1 mT electromagnetic field. J Appl Toxicol 2011; 31: 223–230.
- Koç A, Ünal D, Çimentepe E, Bayrak Ö, Karataş ÖF, Yıldırım ME, Bayrak R, Aydın M. The effects of antioxidants on testicular apoptosis and oxidative stress produced by cell phones. Turk J Med Sci 2013; 43: 131–137.
- Demir T, Gültürk S, Çançalar AD, Durmuş N. Investigation of the effects of magnetic field exposure on febrile seizure latency, seizure duration, and electroencephalographic recordings in a rat febrile convulsion model. Turk J Med Sci 2014; 44: 295–304.
- Gökcimen A, Ozgüner F, Karaöz E, Ozen S, Aydin G. The effect of melatonin on morphological changes in liver induced by magnetic field exposure in rats. Okajimas Folia Anat Jpn 2002; 79: 25–31.
- Tarantino P, Lanubile R, Lacalandra G, Abbro L, Dini L. Post-continuous whole body exposure of rabbits to 650 MHz electromagnetic fields: effects on liver, spleen, and brain. Radiat Environ Biophys 2005; 44: 51–59.
- Watanabe Y, Nakagawa M, Miyakoshi Y. Enhancement of lipid peroxidation in the liver of mice exposed to magnetic field. Ind Health 1997; 35: 285–290.
- Lahijani MS, Tehrani DM, Sabouri E. Histopathological and ultrastructural studies on the effects of electromagnetic fields on the liver of preincubated white leghorn chicken embryo. Electromagn Biol Med, 2009; 28: 391–413.
- Amara S, Abdelmelek H, Garrel C, Guiraud P, Douki T, Ravanat JL, Favier A, Sakly M, Ben Rhouma K. Effect of subchronic exposure to static magnetic field on testicular function in rats. Arch Med Res 2006; 37: 947–952.
- Yurekli AI, Ozkan M, Kalkan T, Saybasili H, Tuncel H, Atukeren P, Gumustas K, Seker S. GSM base station electromagnetic radiation and oxidative stress in rats. Electromagn Biol Med 2006; 25: 177–188.
- Amara S, Abdelmelek H, Garrel C, Guiraud P, Douki T, Ravanat JL, Favier A, Sakly M, Ben Rhouma K. Zinc supplementation ameliorates static magnetic field-induced oxidative stress in rat tissues. Environ Toxicol Pharmacol 2007; 23: 193–197.
- Koyu A, Ozguner F, Yilmaz H, Uz E, Cesur G, Ozcelik N. The protective effect of caffeic acid phenethyl ester (CAPE) on oxidative stress in rat liver exposed to the 900 MHz electromagnetic field. Toxicol Ind Health 2009; 25: 429–434.
- Emre M, Cetiner S, Zencir S, Unlukurt I, Kahraman I, Topcu Z. Oxidative stress and apoptosis in relation to exposure to magnetic field. Cell Biochem Biophys 2011; 59: 71–77.
- Güler G, Seyhan N, Aricioğlu A. Effects of static and 50 Hz alternating electric fields on superoxide dismutase activity and TBARS levels in guinea pigs. Gen Physiol Biophys 2006; 25: 177–193.
- Ghodbane S, Amara S, Garrel C, Arnaud J, Ducros V, Favier A, Sakly M, Abdelmelek H. Selenium supplementation ameliorates static magnetic field-induced disorders in antioxidant status in rat tissues. Environ Toxicol Pharmacol 2011; 31: 100–106.