Canan DEMİRTAŞ YILMAZ, Ahmed HUSSEIN, Özge Tuğçe PAŞAOĞLU, Fatma Ebru DEMİR OFLUOĞLU, Hatice PAŞAOĞLU

The effect of caffeine on oxidative stress in liver and heart tissues of rats

To investigate the effect of caffeine on the levels of malondialdehyde (MDA), nitric oxide (NO), and advanced oxidation protein products (AOPP) in the liver and heart tissues of rats. Materials and methods: The current study included 30 rats, which were divided into 3 groups: a control group and 2 caffeine-treated groups. Group 1 was given caffeine at 30 mg/kg and Group 2 was given caffeine at 100 mg/kg (a high nontoxic dose) for 14 days. Results: MDA and AOPP levels in the liver tissue of the caffeine-treated groups decreased significantly as a result of the dose. MDA and AOPP levels in the heart tissue also decreased, but this effect was not significantly affected by the dose. NO levels in the liver tissue of the caffeine-treated groups were higher than those in the control group; in the heart tissues, however, NO levels were not significantly affected by caffeine. Conclusion: These results show that the short-term consumption of 2 different doses of caffeine may potentially protect against oxidative stress in the liver. This effect is related to the dose of caffeine in the liver tissue. Further studies will be needed to discover the mechanisms responsible for these findings.

Anahtar Kelimeler:

Key words: Caffeine, malondialdehyde, AOPP, nitric oxide

The effect of caffeine on oxidative stress in liver and heart tissues of rats

Keywords:

Key words: Caffeine, malondialdehyde, AOPP, nitric oxide,

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Kolaylı S, Ocak M, Küçük M, Abbasoğlu R. Does caff eine bind to metal ions? Food Chemistry 2004; 84: 383-8.
Al Deeb SA, Al Moutaery KA, Arshaduddin M, Biary N, Tariq M. Eff ect of acute caff eine on severity of harmaline induced tremor in rats. Neuroscience Letter 2002; 325: 216-8. 3. Barbier A. Caff eine protects against Parkinson’s disease? Trends Pharmacology Science 2001; 22: 500.
Graham T. Caff eine and exercise metabolism, endurance and performance. Sports Medicine 2001; 31: 785-807.
Fredholm BB, Battig K, Holmen J, Nehling A, Zvartau EE. Actions of caff eine in the brain with special reference to factors that contribute to its widespread use. Pharmacology Reviews 1999; 51: 84-125.
Jewett S, Eddy L, Hochstein P. Is the autoxidation of catecholamines involved in ischemia-reperfusion injury? Free Radical Biology & Medicine 1989; 6: 185-8.
Vistisen K, Poulsen H, Loft S. Foreign compound metabolism capacity in man measured from metabolites of dietary caff eine. Carcinogenesis 1992, 13: 1561-8. 8. Dianzani M, Muzio G, Biocca M, Canuto R. Lipid peroxidation in fatty liver induced by caff eine in rats. International Journal of Tissue Reactions 1991; 13: 79-85.
Krisko A, Kveder M, Pifat G. Eff ect of caff eine to oxidation susceptibility of human plasma low density lipoproteins. Clinica Chimica Acta 2005; 355: 47-53.
Nikolic J, Bjelakovic G, Stojanovic I. Eff ect of caff eine on metabolism of L-arginine in the brain. Molecular and Cellular Biochemistry 2003; 244: 125-8.
Çakatay U, Telci A, Kayalı R, Tekeli F, Akçay T, Sivas A. Relation of aging with oxidative protein damage parameters in the rat skeletal muscle. Clinical Biochemistry 2003; 36: 51-5.
Mori M, Gotoh T. Regulation of nitric oxide production by arginine metabolic enzymes. Biochemical and Biophysical Research Communications 2000; 275: 715-9.
Paşaoğlu H, Bulduk G, Oğüs E, Paşaoğlu A, Onalan G. Nitric oxide, lipid peroxides, and uric acid levels in pre-eclampsia and eclampsia. Tohoku Journal of Experimental Medicine 2004; 202: 87-92.
Amudha G, Josephine A, Sudhahar V, Varalakshmi P. Protective eff ect of lipoic acid on oxidative and peroxidative damage in cyclosporine A-induced renal toxicity. International Immunopharmacology 2007; 7: 1442-9.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurements with the folin phenol reagent. Th e Journal of Biological Chemistry 1951; 193: 265-75.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95: 351-8.
Witko-Sarsat V, Friedlander M, Khoa TN, Capeillère-Blandin C, Nguyen AT, Canteloup S et al. Advanced oxidation protein products as novel mediators of infl ammation and monocyte activation in chronic renal failure. Th e Journal of Immunology 1998; 161: 2524-32.
Davis J, Zhao Z, Stock HS, Mehl K, Buggy J, Hand G. Central nervous system eff ects of caff eine and adenosine on fatigue. American Journal of Physiology Regulatory Integrative and Comparative Physiology 2003; 284: R399-404.
Greer F, Friars D, Graham TE. Comparison of caff eine and theophylline ingestion: exercise metabolism and endurance. Journal of Applied Physiology 2000; 89: 1837-44.
Savoca MR, Evans CD, Wilson ME, Harshfi eld GA, Ludwig DA. Th e association of caff einated beverages with blood pressure in adolescents. Archives of Pediatrics & Adolescent Medicine 2004; 158: 473-7.
Dianzani MU, Muzio G, Biocca ME, Canuto RA. Lipid peroxidation in fatty liver induced by caff eine in rats. International Journal of Tissue Reactions 1991; 13: 79-85.
Al Moutaery K, Al Deeb S, Ahmad Khan H, Tariq M. Caff eine impairs short-term neurological outcome aft er concussive head injury in rats. Neurosurgery 2003; 53: 704-11.
Karas M, Chakrabarti SK. Infl uence of caff eine on allyl alcohol-induced hepatotoxicity in rats. In vivo study. Journal of Environmental Pathology, Toxicology and Oncology 2001; 20: 141-54.
Mukhopadhyay S, Mondal A, Poddar MK. Chronic administration of caff eine: eff ect on the activities of hepatic antioxidant enzymes of Ehrlich ascites tumor-bearing mice. Indian Journal of Experimental Biology 2003; 41: 283-9.
Lee J, Ha JH, Kim S, Oh YW, Kim SW. Caff eine decreases the expression of Na+/K+-ATPase and the type 3 Na+/ H+ exchanger in rat kidney. Clinical and Experimental Pharmacology and Physiology 2002; 29: 559-63.
Aronowski J, Strong R, Shirzadi A, Grotta JC. Ethanol plus caff eine (caff einol) for treatment of ischemic stroke. Stroke 2003; 34: 1246-51.
Strong R, Grotta JC, Aronowski J. Combination of low dose ethanol and caff eine protects brain from damage produced by focal ischemia in rats. Neuropharmacology 2000; 39: 515-22.
Alasehirli B, Cekmen M, Nacak M, Balat A. Eff ects of caff eine on placental total nitrite concentration: A 21-day, vehicle- controlled study in rats. Current Th erapeutic Research 2005; 66: 130-7.
Strong R, Grotta JC, Aronowski J. Combination of low dose ethanol and caff eine protects brain from damage produced by focal ischemia in rats. Neuropharmacology 2000; 39: 515-22.
Kamat J, Boloor K, Devasagayam T, Jayashree B, Kesavan P. Diff erential modifi cation by caff eine of oxygen-dependent and independent eff ects of gamma-irradiation on rat liver mitochondria. International Journal of Radiation Biology 2000; 76: 1281-8.
Yukawa GS, Mune M, Otani H, Tone Y, Liang XM, Iwahashi H et al. Eff ects of coff ee consumption on oxidative susceptibility of low-density lipoproteins and serum lipid levels in humans. Biochemistry (Moscow) 2004; 69: 70-4.
Kim SW, Lee J, Pack YW, Kang DG, Choi KC. Decreased nitric oxide synthesis in rats with chronic renal failure. Journal of Korean Medical Science 2000; 15: 425-30.
Bruce C, Yates DH, Th omas PS. Caff eine decreases exhaled nitric oxide. Th orax 2002; 57: 361-3.
Corsetti G, Pasini E, Assanelli D, Saligari E, Adobati M, Bianchi R. Acute caff eine administration decreased NOS and Bcl2 expression in rat skeletal muscles. Pharmacology Research 2007; 55: 96-103.
Kayir H, Uzbay IT. Evidence for the role of nitric oxide in caff eine-induced locomotor activity in mice. Psychopharmacology 2004; 172: 11-5.
Hashiguchi W, Nagatomo I, Akasaki Y, Uchida M, Tominaga M, Takigawa M. Infl uences of caff eine to nitric oxide production and zonisamide concentration in the brain of seizure- susceptible EL mice. Psychiatry and Clinical Neurosciences 2001; 55: 319-24.
Ofl uoglu E, Pasaoglu H, Pasaoglu A. Th e eff ects of caff eine on L-arginine metabolism in the brain of rats. Neurochemical Research 2009; 34: 395-9.
Burkard N, Rokita AG, Kaufmann SG, Hallhuber M, Wu R, Hu K et al. Conditional neuronal nitric oxide synthase overexpression impairs myocardial contractility. Circulation Research 2007; 100: e32-e44.
Steppan J, Ryoo S, Schuleri KH, Gregg C, Hasan RK, White AR et al. Arginase modulates myocardial contractility by a nitric oxide synthase 1-dependent mechanism. Th e Proceedings National Academy of Sciences USA 2006; 103: 4759-64.
Khan SA, Lee K, Minhas KM, Gonzales DR, Raju SV, Tejani AD et al. Neuronal nitric oxide synthase negatively regulates xanthine oxidoreductase inhibition of cardiac excitation contraction coupling. Th e Proceedings National Academy of Sciences USA 2004, 45: 15944-8.
Corsetti G, Pasini E, Assanelli P, Bianchi R. Eff ects of acute caff eine administration on NOS and Bax/Bcl2 expression in the myocardium of rat. Pharmacology Research 2008, 57: 19- 25.
Perrone S, Mussap M, Longini M, Fanos V, Bellieni CV, Proietti F et al. Oxidative kidney damage in preterm newborns during perinatal period. Clinical Biochemistry 2007; 40: 656-60.