Akif CİNEL, Deniz FİLİNTE, Serdar TÜRKYILMAZ, Murat ALPER, Ethem ALHAN, Mehmet Cengiz ERÇİN, Birgül KURAL VANİZOR

The effects of p53 inhibition using pifithrin-a on acute necrotizing pancreatitis in rats

Amaç: p53 geninin akut pankreatit seyri esnasında rolü bilinmemektedir. Çalışmanın amacı pifi thrin-α (PFA) kullanarak sağlanan p53 inbisyonunun glikodeoksikolik asitle oluşturulan akut nekrotizan pankreatite(ANP) etkisi araştırmaktır. Yöntem ve gereç: Sıçanlar sham+salin, sham+PFA, ANP+salin ve ANP+PFA olmak üzere 4 gruba ayrıldı. ANP glikodeoksikolik asitle oluşturuldu. Pankreatik injuri, apoptozis, mortalite, sistemik kardiovasküler parametreler, fonksiyonel kapiller dansite (FCD), böbrek, karaciğer fonksiyonları ve pankreas ve akciğer dokusunda bazı enzim belirteçleri incelendi. Bulgular: Pankreatit indüksiyonu mortaliteyi, pankreatik nekrozu, apopitozisi, serum amilaz, üre, alanin aminotransferazı (ALT), interlökin(IL)-6, bronkoalveolar lavajda laktik dehidrogenazı (LDH), pankreas ve akciğer dokularında myeloperoksidaz (MPO), malondialdehit (MDA) önemli olarak artırdı. Serum kalsiyum, kan basıcı, idrar debisi, pO2, FCD azalttı. PFA kullanılması bu değişikleri değiştirmedi. Sonuç: PFA kullanılması pankreatit seyrini etkilemedi. Dolayısıyla PFA nın akut pankreatit tedavisinde yeri olmadığını düşündürmektedir.

Pifithrin-a ile oluşturulan p53 inhibisyonunun sıçanlarda akut nekrotizan pankreatite etkileri

Aim: To investigate the effects of p53 inhibition using pifithrin-α (PFA) on acute necrotizing pancreatitis (ANP) induced by glycodeoxycholic acid in rats. The role of p53 during acute pancreatitis is unclear. Materials and methods: Rats were divided into 4 groups, 2 of which were initially given only saline (the sham group) and 2 of which were given glycodeoxycholic acid in order to induce ANP. Each group was then identified according to treatment plan: sham+saline, sham+PFA, ANP+saline, or ANP+PFA. Th e study then investigated the extent of acinar cell injury, apoptosis, mortality, systemic cardiorespiratory variables, functional capillary density (FCD), renal/hepatic functions, and changes in some enzyme markers for pancreatic and lung tissue during ANP in rats. Results: The induction of ANP resulted in a significant increase in mortality rate, pancreatic necrosis, apoptosis, serum activity of amylase, alanine aminotransferase (ALT), interleukin (IL)-6, lactate dehydrogenase (LDH) in bronchoalveolar lavage (BAL) fl uid, serum concentration of urea, and tissue activity of myeloperoxidase (MPO) and malondialdehyde (MDA) in the pancreas and lung. In addition, ANP induction caused a signifi cant decrease in concentrations of calcium, blood pressure, urine output, pO2, and functional capillary density (FCD). The use of PFA did not alter these changes. Conclusion: PFA demonstrated no effect on the course of ANP in rats. Th erefore, it has no value in the treatment of acute pancreatitis.

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