The effect of caffeic acid phenethyl ester on bacterial translocation and intestinal damage after intestinal obstruction
Intestinal obstruction (IO) induces bacterial translocation due to failure of the intestinal barrier function. Following bacterial overgrowth, its degradation products play a decisive role in the development of systemic septic complications. The aim of this study was to evaluate the effects of caffeic acid phenethyl ester (CAPE) on bacterial translocation and intestinal damage in an IO model in rats. Materials and methods: Complete IO was created in the distal ileum of rats by a single 4-0 silk suture. A total of 21 Wistar albino rats were randomized into 3 groups: Group 1, Sham (n = 7); Group 2, IO (n = 7); Group 3, IO + CAPE (n = 7). Group 3 received a 10 μmol kg-1 dose of CAPE intraperitoneally. This treatment was continued for 3 days (2 days before surgery and 1 day after surgery). Samples of mesenteric lymph nodes (MLN), liver, and segmental ilea were obtained 24 h after the mechanical bowel obstruction, both for biochemical analysis and microbiological examination. Results: The most common bacteria cultured from the liver and MLN of these animals were Escherichia coli, Proteus mirabilis, and Enterococcus spp. In the CAPE-treated rats, the malondialdehyde (MDA) and adrenomedullin levels were significantly lower than in the IO group (P < 0.001). The reduced glutathione (GSH) and catalase (CAT) levels of the ileum were found to be significantly higher in the CAPE-treated rats than those in the IO group (P < 0.001). Conclusion: These results have shown that CAPE may have protective effects against bacterial translocation and intestinal oxidative damage in mechanical IO. More experimental studies are needed to explain the exact mechanism of this beneficial effect.
The effect of caffeic acid phenethyl ester on bacterial translocation and intestinal damage after intestinal obstruction
Intestinal obstruction (IO) induces bacterial translocation due to failure of the intestinal barrier function. Following bacterial overgrowth, its degradation products play a decisive role in the development of systemic septic complications. The aim of this study was to evaluate the effects of caffeic acid phenethyl ester (CAPE) on bacterial translocation and intestinal damage in an IO model in rats. Materials and methods: Complete IO was created in the distal ileum of rats by a single 4-0 silk suture. A total of 21 Wistar albino rats were randomized into 3 groups: Group 1, Sham (n = 7); Group 2, IO (n = 7); Group 3, IO + CAPE (n = 7). Group 3 received a 10 μmol kg-1 dose of CAPE intraperitoneally. This treatment was continued for 3 days (2 days before surgery and 1 day after surgery). Samples of mesenteric lymph nodes (MLN), liver, and segmental ilea were obtained 24 h after the mechanical bowel obstruction, both for biochemical analysis and microbiological examination. Results: The most common bacteria cultured from the liver and MLN of these animals were Escherichia coli, Proteus mirabilis, and Enterococcus spp. In the CAPE-treated rats, the malondialdehyde (MDA) and adrenomedullin levels were significantly lower than in the IO group (P < 0.001). The reduced glutathione (GSH) and catalase (CAT) levels of the ileum were found to be significantly higher in the CAPE-treated rats than those in the IO group (P < 0.001). Conclusion: These results have shown that CAPE may have protective effects against bacterial translocation and intestinal oxidative damage in mechanical IO. More experimental studies are needed to explain the exact mechanism of this beneficial effect.
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- Luck H. Catalase. In: Bergmayer H, editor. Methods of
- enzymatic analysis. New York: Verlag Chemie, Weinheim and
- Academic Press; 1963. p.885-88.
- Lawrance RA, Burk RF, Berg RD, Garlington AW. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Comm 1976; 71: 952-8.
- Theodorus P, Akerboom M, Sies H. Assay of glutathione, glutathione disulfide and glutathione mixed disulfides in biological samples. Methods Enzymol 1981; 77: 373- 83.
- Buege AJ, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-10.
- Lowry O, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurements with the Folin phenol reagent. J Biol Chem 1951; 193: 265-75.
- Sato K, Hirata Y, Imai T, Iwashina M, Marumo F. Characterization of immunoreactive adrenomedullin in human plasma and urine. Life Sci 1995; 57: 189-94.
- Berg RD, Garlington AW. Translocation of certain indigenous bacteria from the gastrointestinal tract to mesenteric lymph nodes and other organs in a mouse model. Infect Immun 1979; 23: 403-11.
- Deitch EA. Simple intestinal obstruction causes bacterial translocation in man. Arch Surg 1989; 124: 699-701.
- Antequera R, Bretana A, Cirac A, Brito A, Romera MA, Zapata R. Disruption of the intestinal barrier and bacterial translocation in an experimental model of intestinal obstruction. Acta Cient Venez 2000; 51: 18-26.
- Gurleyik G, Ozturk E, Adaleti R, Pembe G, Guran M, Peker O, Saglam A. Effects of prostaglandin E1 and E2 analogues on mucosal injury-induced, and on bacterial translocation promoted by experimental intestinal obstruction. J Invest Surg 2004; 17: 127-34.
- Kocdor MA, Kocdor H, Gulay Z, Gokce O. The effects of pentoxifylline on bacterial translocation after intestinal obstruction. Shock 2002; 18: 148-51.
- Kitamura K, Kangawa K, Kawamoto M, Ichiki Y, Nakamura S, Matsuo H et al. Adrenomedullin: a novel hypotensive peptide isolated from human pheochromocytoma. Biochem Biophys Res Commun 1993; 192: 553-60.
- Kangawa K, Kitamura K, Minamino N, Eto T, Matsuo H. Adrenomedullin: a new hypotensive peptide. J Hypertension 1996; 14: 105-10.
- Hirata Y, Mitaka C, Sato K, Nagura T, Tsunoda Y, Amaha K, Marumo F. Increased circulating adrenomedullin, a novel vasodilatory peptide, in sepsis. J Clin Endocrinol Metab 1996; 81: 1449-53.
- Ueda S, Nishio K, Minamino N, Kubo A, Akai Y, Kangawa K et al. Increased plasma levels of adrenomedullin in patients with systemic inflammatory response syndrome. Am J Respir Crit Care 1999; 160: 132-6.
- Evereklioglu C, Yurekli M, Er H, Ozbek E, Hazneci E, Cemken M et al Increased plasma adrenomedullin levels in patients with Behçet's disease. Dermatology 2000; 201: 312-5.
- Garayoa M, Martinez A, Lee S, Pio R, An WG, Neckers L et al. Hypoxia-inducible factor-1 (HIF-1) up-regulates adrenomedullin expression in human tumor cell lines during oxygen deprivation: a possible promotion mechanism of carcinogenesis. Mol Endocrinol 2000; 14: 848-62.
- Chun TH, Itoh H, Saito T, Yamahara K, Doi K, Mori Y et al. Oxidative stress augments secretion of endothelium-derived relaxing peptides, C-type natriuretic peptide and adrenomedullin. J Hypertens 2000; 18: 575-80.
- Zaks-Zilberman M, Salkowski CA, Elsasser T, Cuttitta F, Vogel SN. Induction of adrenomedullin mRNA and protein by lipopolysaccharide and paclitaxel (Taxol) in murine macrophages. Infect Immun 1998; 66: 4669-75.
- Parlakpinar H, Sahna E, Ozer MK, Ozugurlu F, Vardi N, Acet A. Physiological and pharmacological concentrations of melatonin protect against cisplatin-induced acute renal injury. J of Pineal Res 2002; 33: 161-6.
- Bilen BT, Kilinç H, Alaybeyoglu N, Celik M, Iraz M, Sezgin N et al. Effect of caffeic acid phenethyl ester on survival of axial pattern flaps in rats with ischaemia-reperfusion injuries. Scand J Plast Reconstr Surg. and Hand Surg 2006; 40: 73-8.
- Akyildiz M, Ersin S, Oymaci E, Dayangac M, Kapkac M, Alkanat M. Effects of somatostatin analogues and vitamin C on bacterial translocation in an experimental intestinal obstruction model of rats. J Invest Surg 2000; 13: 169-73.
- Park JH, Lee JK, Kim HS, Chung ST, Eom JH, Kim KA et al. Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice. Int Immunopharmacol 2004; 4: 429-36.