Kıvanç ERGEN, Barış AYDIL, Mehmet YALTIRIK, Koçak Hülya BERBEROĞLU

Sjögren's syndrome: A study of salivary electrophoresis

Many diagnostic tests exist to assess the salivary and lacrimal involvement in Sjögren's syndrome (SS), but there is still disagreement. The purpose of this study was to examine salivary proteins in patients with primary Sjögren's syndrome and healthy control subjects by the polyacrylamide gel electrophoresis, as well as the applicability of the method. The polyacrylamide gel electrophoresis, applied in our examinations, is a simple and rapid method, requiring only a minute amount of saliva for the analysis. Saliva were collected from patients suffering from primary Sjögren's syndrome (n = 10) and a control group (n = 10). All saliva samples were stored at -70 °C until use. The diagnosis of Sjögren's syndrome was verified by a biopsy of the minor glands of the lower lip in the patient group. Most of the patients with Sjögren's syndrome exhibited an electrophoretic profile that was different from healthy control subjects. Electrophoresis systems are most reliable for Sjögren’s syndrome. Our study suggests that the salivary electrophoresis are most reliable for Sjögren's syndrome. Salivary electrophoresis can provide a simple, noninvasive, inexpensive, and comfortable method for the diagnosis of Sjögren's syndrome.

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1. Rhodus LN. Sjögren's Syndrome Oral Medicine. Quintessence Int 30: 689-99, 1999.
2. Al - Hashimi I, Haghıghat N, Fox PC. Salivary Electrophoresis in the Diagnosis of Sjögren Syndrome. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85: 542-547, 1998.
3. Beeley A J. Clinical .Applications of Electrophoresis of Human Salivary Proteins. J Chromat, 569: 261-280, 1991.
4. Moody M, Zipp M, Al-Hashimi 1. Salivary Anti-Spectrin . Autoantibodies in Sjögren's Syndrome. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91: 322-327, 2001.
5. Scully C. Sjögren's Syndrome: Clinical and laboratory features, immunopathogenesis, and management. Oral Surg Oral Med Oral Pathol, 62: 510-523, 1986.
6. Fischer CJ, Wyshak GH, Weisberger D. Sjögren's syndrome electrophoretic and immunological observations on serum and salivary proteins of man. Archs oral Biol, 13: 257-270, 1968.
7. Vitali C, Moutsopoulos HM, Bombardieri S. The European Community Study Group on Diagnostic Criteria for Sjögren's Syndrome. Sensitivity and Specificity of Tests For Ocular and Oral Involvement in Sjögren's Syndrome. Ann Rheum Dis, 53: 637-647, 1994.
8. Spât BE, Puskas M, Rajki J. Electrophoretic Protein Pattern of Human Parotid Saliva in Sjögren's Syndrome and Sialosis. Acta Otolaryngol, 90: 275-282, 1980.
9. Smith PK, Krohn RI, Hermanson GT et al. Measurement of Protein Using Bicinchoninic Acid. Anal Biochem 150: 76-85, 1985.
10. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-85, 1970.
11. Moody M, Zipp MM, Yasbin L et al. The Effect of Parotid salivary Flow Rate on the Levels of Salivary Antimicrobial Proteins in Patients with Sjögren's Syndrome. Oral Med Clin Res, 30: 700-705, 1999.
12. Beeley AJ, Khoo SK, Lamey JP. Two Dimensional Electrophoresis of Human Parotid Salivary Proteins From Normal and Connective Tissue Disorder Subjects Using Immobilized pH Gradients. Electrophoresis 12: 493-499, 1991.