Sevda Eliş YILDIZ, Mümtaz NAZLI, Gökhan NUR

Immunohistochemical distribution and gene expression of transforming growth factor alpha in ovarian tissue of rats treated with capsaicin in puberty

In this study, the effects of capsaicin on the immunohistochemical distribution and gene expression of transforming growth factor alpha (TGF-a) in ovarian tissue of rats at the pubertal age were examined. Materials and methods: Rats (50 days old) were divided into experimental, alimentation, and sham groups. The experimental group was injected with 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture) subcutaneously every day for a period of 1 week. The alimentation group received 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture). The sham group was injected with a solution of 10% ethanol and 1% Tween in a mixture of 80% distilled water. Body weights and ovary weights were compared within the groups. Triple staining methods were used for the histological examinations. The tissue localization of TGF-a was analyzed using an immunohistochemical method and gene expression level was determined using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Results: No difference in the structure of the 3 groups was observed histologically. Immunohistochemically, TGF-a immunoreactivity was detected in the primordial follicles, primary follicles, theca cells, and interstitial cells at a medium density in all groups. The effect of capsaicin on TGF-a with mRNA was not found to be significantly different among the 3 groups. Conclusion: The results showed that capsaicin had no effects on ovaries at different developmental stages.

Anahtar Kelimeler:

Key words: Capsaicin, TGF-a, ovary, RT-PCR, immunohistochemistry, rat

Immunohistochemical distribution and gene expression of transforming growth factor alpha in ovarian tissue of rats treated with capsaicin in puberty

Keywords:

Key words: Capsaicin, TGF-a, ovary, RT-PCR, immunohistochemistry, rat,

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