Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

One of the most common psychiatric disorders in children is attention-deficit/hyperactivity disorder, which is treated extensively by methylphenidate. This study investigates the assessment of the effects of methylphenidate on histopathologic and histomorphometric changes of the testes and serum levels of gonadotropin in mice. Materials and methods: In this study, 36 adult male mice were used. After determining their body weights, the animals were divided randomly into 2 experimental groups and 1 control group. The experimental groups received methylphenidate (2 and 10 mg kg-1 day-1) via gavage for a period of 40 days. After evaluation of body weight, general anesthesia was used for taking blood samples from the heart in order to measure testosterone and levels of gonadotropin in serum. For the purpose of weighing the bodies and measuring the thickness of the germinal epithelium, the testes were removed and the possibility of any pathological changes was considered. Results: The results showed that methylphenidate could decrease the thickness of the germinal epithelium and body weight significantly, and could increase the levels of spermatogonia and serum gonadotropins and testosterone. Histopathological changes were also seen for vascular dilatation and congestion in interstitial tissue. Conclusion: Our findings demonstrated that administration of methylphenidate in adulthood may have an effect on spermatogenesis due to the influence of gonadotropin hormones on testis function.

Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

One of the most common psychiatric disorders in children is attention-deficit/hyperactivity disorder, which is treated extensively by methylphenidate. This study investigates the assessment of the effects of methylphenidate on histopathologic and histomorphometric changes of the testes and serum levels of gonadotropin in mice. Materials and methods: In this study, 36 adult male mice were used. After determining their body weights, the animals were divided randomly into 2 experimental groups and 1 control group. The experimental groups received methylphenidate (2 and 10 mg kg-1 day-1) via gavage for a period of 40 days. After evaluation of body weight, general anesthesia was used for taking blood samples from the heart in order to measure testosterone and levels of gonadotropin in serum. For the purpose of weighing the bodies and measuring the thickness of the germinal epithelium, the testes were removed and the possibility of any pathological changes was considered. Results: The results showed that methylphenidate could decrease the thickness of the germinal epithelium and body weight significantly, and could increase the levels of spermatogonia and serum gonadotropins and testosterone. Histopathological changes were also seen for vascular dilatation and congestion in interstitial tissue. Conclusion: Our findings demonstrated that administration of methylphenidate in adulthood may have an effect on spermatogenesis due to the influence of gonadotropin hormones on testis function.

Kaynakça

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Kaynak Göster

Bibtex @ { tbtkmedical146661, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2014}, volume = {44}, pages = {554 - 559}, doi = {10.3906/muh-1201-6}, title = {Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice}, key = {cite}, author = {Fazelıpour, Simin and Tootıan, Zahra and Saremı, Zahra GHAHRI and Shafıı, Minoo and Sheıbanı, Mohammad Taghi and Kıaeı, Seyed Babak and Kıumarsı, Mehrnush and Assadı, Fardin} }
APA Fazelıpour, S , Tootıan, Z , Saremı, Z , Shafıı, M , Sheıbanı, M , Kıaeı, S , Kıumarsı, M , Assadı, F . (2014). Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice . Turkish Journal of Medical Sciences , 44 (4) , 554-559 . DOI: 10.3906/muh-1201-6
MLA Fazelıpour, S , Tootıan, Z , Saremı, Z , Shafıı, M , Sheıbanı, M , Kıaeı, S , Kıumarsı, M , Assadı, F . "Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice" . Turkish Journal of Medical Sciences 44 (2014 ): 554-559 <
Chicago Fazelıpour, S , Tootıan, Z , Saremı, Z , Shafıı, M , Sheıbanı, M , Kıaeı, S , Kıumarsı, M , Assadı, F . "Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice". Turkish Journal of Medical Sciences 44 (2014 ): 554-559
RIS TY - JOUR T1 - Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice AU - Simin Fazelıpour , Zahra Tootıan , Zahra GHAHRI Saremı , Minoo Shafıı , Mohammad Taghi Sheıbanı , Seyed Babak Kıaeı , Mehrnush Kıumarsı , Fardin Assadı Y1 - 2014 PY - 2014 N1 - doi: 10.3906/muh-1201-6 DO - 10.3906/muh-1201-6 T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 554 EP - 559 VL - 44 IS - 4 SN - 1300-0144-1303-6165 M3 - doi: 10.3906/muh-1201-6 UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Medical Sciences Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice %A Simin Fazelıpour , Zahra Tootıan , Zahra GHAHRI Saremı , Minoo Shafıı , Mohammad Taghi Sheıbanı , Seyed Babak Kıaeı , Mehrnush Kıumarsı , Fardin Assadı %T Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice %D 2014 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 44 %N 4 %R doi: 10.3906/muh-1201-6 %U 10.3906/muh-1201-6
ISNAD Fazelıpour, Simin , Tootıan, Zahra , Saremı, Zahra GHAHRI , Shafıı, Minoo , Sheıbanı, Mohammad Taghi , Kıaeı, Seyed Babak , Kıumarsı, Mehrnush , Assadı, Fardin . "Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice". Turkish Journal of Medical Sciences 44 / 4 (Mayıs 2014): 554-559 .
AMA Fazelıpour S , Tootıan Z , Saremı Z , Shafıı M , Sheıbanı M , Kıaeı S , Kıumarsı M , Assadı F . Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice. Turkish Journal of Medical Sciences. 2014; 44(4): 554-559.
Vancouver Fazelıpour S , Tootıan Z , Saremı Z , Shafıı M , Sheıbanı M , Kıaeı S , Kıumarsı M , Assadı F . Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice. Turkish Journal of Medical Sciences. 2014; 44(4): 554-559.
IEEE S. Fazelıpour , Z. Tootıan , Z. Saremı , M. Shafıı , M. Sheıbanı , S. Kıaeı , M. Kıumarsı ve F. Assadı , "Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice", Turkish Journal of Medical Sciences, c. 44, sayı. 4, ss. 554-559, May. 2014, doi:10.3906/muh-1201-6
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

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