Jamshid MOHAMMADI, Khalil SAADIPOUR, Hamdollah DELAVIZ, Bahram MOHAMMADI

Anti-diabetic effects of an alcoholic extract of Juglans regia in an animal model

Many traditional treatments have been recommended in the alternative system of medicine for treatment of diabetes mellitus. Juglans regia is one of the medicinal plants used in traditional Iranian medicine as a treatment for diabetes, but little scientific documentation supports its antidiabetic action. The purpose of this study was to investigate antidiabetic effect of J. regia leaves in type 1 diabetes induced animal model. Materials and methods: Four groups of animals were selected. Group I animals were fed a normal diet. Animals of groups II, III, and IV received streptozotocin. Animals of groups III and IV were treated with J. regia leaf extract 200 and 400 mg/kg body weight for 28 days. The various parameters studied included body weight, blood glucose, triglycerides, cholesterols, LDL, VLDL, HDL, insulin, and glycated hemoglobin in all groups. Results: Treatment with the J. regia extracts resulted in a significant decrease in blood glucose, glycosylated hemoglobin, LDL, triglyceride, and total cholesterol, and a significant increase in insulin and HDL level. Conclusion: The plant extract is capable of ameliorating hyperglycemia in streptozotocin-induced diabetic rats and is a potential source for active agent(s) for diabetes mellitus. It is concluded that J. regia leaf extract can be potentially used to control type 1 diabetes.

Anahtar Kelimeler:

Key words: Juglans regia, streptozotocin, insulin, diabetes mellitus, glucose, triglyceride, cholesterol

Anti-diabetic effects of an alcoholic extract of Juglans regia in an animal model

Keywords:

Key words: Juglans regia, streptozotocin, insulin, diabetes mellitus, glucose, triglyceride, cholesterol,

PDF

___

Huang THW, Peng G, Kota BP, Li GQ, Yamahara J, Roufogalis BD, Li Y. Anti-diabetic action of Punica granatum fl ower extract: activation of PPAR-c and identifi cation of an active component. Toxicol Appl Pharmacol 2005; 207: 160-169. 2. Wild SG, Roglic A, Green R, King H. Global prevalence of diabetes. Estimated for the year 2000 and projection for 2030. Diabetes Care 2004; 27: 1047-1054. 3. Pari L, Uma MJ. Hypoglycemic eff ect of Musa sapientum L. in alloxan-induced diabetic rats. J Ethnopharmacol 1999; 68: 321-325.
Urger RH, Foster DW. Diabetes mellitus. In: Wilson, JD., Foster, DW., Kronenserg, HM, Larson PR (Eds.). Williams’s Textbook of Endocrinology. WB Saunders, Philadelphia, 2001; pp. 973-1060.
Muhlhauser I, Sawicki PT, Blank M, Overmann H, Bender R, Berger M. Prognosis of persons with type 1 diabetes on intensifi ed insulin therapy in relation to nephropathy. J Inter Med 2000; 248: 333-341.
Sharma SB, Nasir A, Prabhu KM, Murthy PS, Dev G. Hypoglycaemic and hypolipidemic eff ect of ethanolic extract of seeds of Eugenia jambolana in alloxan-induced diabetic rabbits. J Ethnopharmacol 2003; 85: 201-206.
Piedrola G, Novo E, Escober F, Garcia-Robles R. White blood cell count and insulin resistance in patients with coronary artery disease. Ann Endocrinol. (Paris). 2001; 62: 7-10.
Yaryura-Tobias JA, Pinto A, Neziroglu F. Anorexia nervosa, diabetes mellitus, brain atrophy, and fatty liver. Inter J Etiol Disorders 2001; 30: 350-353.
Singh RP, Padmavathi B, Rao AR. Modulatory infl uence of Adhatoda vesica (Justica adhatoda) leaf extract on the enzyme of xenobiotic metabolism, antioxidant status and lipid peroxidation in mice. Molecular and Cellular Biochem 2000; 213: 99-109.
Fallah Hosseini H, Fakhrzadeh H, Larigani B, Sheikh Samani AH. Review on therapeutic plant used in diabetes. J Medicinal Plants (Persian) 2006; 5: 1-8.
Hu X, Sato J, Oshida Y, Yu M, Bajotto G, Sato Y. Eff ect of Gosha-jinki-gan (Chinese herbal medicine: Niuche-sen-qi- wan) on insulin resistance in streptozotocin induced diabetic rats. Diabetes Res Clin Pract 2003; 59: 103-111.
Amaral JS, Seabra, RM, Andrade, PB. Phenolic profi le in the quality control of walnut (Juglans regia L.) leaves. Food Chemistry 2004; 88: 373-379.
Fatima SS, Rajasekhar MD, Kumar KV, Kumar MTS, Babu KR, Rao C. Antidiabetic and antihyperlipidemic activity of ethyl acetate:Isopropanol (1:1) fraction of Vernonia anthelmintica seeds in Streptozotocin induced diabetic rats. Food and Chemical Toxicology 2010; 48: 495-501.
Trinder, P. Determination of blood glucose using an oxidase- peroxidase system with a non-carcinogenic chromogen. J. Clin. Pathol. 1969; 22: 158-161.
Parekh AC, Jung DH. Cholesterol determination with ferric acetate uranyl acetate and sulphuric acid-ferrous sulphate reagents. Analytical Chemistry. 1970; 42: 1423-1427.
Willey DG, Rosenthal MA, Caldwell S. Glycosylated hemoglobin and plasma glycoprotein assay by affi nity chromatography. Diabetologia. 1984; 27: 56-61.
Fossati P, Lorenzo P. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinl. Chem. 1982; 28: 2077-2080.
Burstein M, Scholnick HR, Morgin R. Rapid method for the isolation of lipoprotein from human serum by precipitation with polyanion. J. Lipid Res. 1970; 11: 1583-1586.
Friedwald WT, Levy KJ, Frederickson DS. Estimation of concentration of LDL in plasma without use of preparative ultracentrifuge. Clin. Chem. 1972; 18: 499-502.
Lemus I, Garcia R, Delvillar E, Knop G. Hypoglycemic activity of four plants used in Chilean popular medicine. Phytother Res 1999; 13: 91-94.
Sachdewa A, Khemani LD. Eff ect of Hibiscus rosa sinensis Linn ethanol fl ower extract on blood glucose and lipid profi le in streptozotocin induced diabetes in rats. J Ethropharmacol 2003; 89: 61-66.
Andallu B, Varadacharyulu N. Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin diabetic rats. Clinica Chimica Acta 2003; 338: 3-10.
Mohammadi J, Naik PR. Evaluation of hypoglycemic eff ect of Morus alba in an animal model. Indian J Pharmacol 2008; 40(1): 15-18.
Komeili GH, Bagheri H. Eff ects of walnut leaf aqueous extract on blood sugar and lipids in male diabetic rats. Saudi Med J 2008; 29(9): 1350-62.
Andallu B, Suryakantham V, Lakshmi B, Reddy GK. Eff ect of mulberry (Morus indica L.) therapy on plasma and erythrocyte membrane lipids in patients with type 2 diabetes. Clinica. Chimica. Acta. 2001; 314: 47-53.
Asgary S, Parkhideh S, Solhpour A, Madani H, Mahzouni P, Rahimi P. Eff ect of ethanolic extract of Juglans regia L. on blood sugar in diabetes-induced rats. J Med Food. 2008; 11(3): 533-8.
Tavakkoli A, Kimiagar M, Valaei N. Eff ect of Iranian walnut on blood lipids in menopause women. J of Mazandaran University of Medical Sciences 2004; 44: 21-32.
Laakso M, Sarlund H, Mykkanen L. Insulin resistance is associated with lipid and lipoprotein abnormalities in subjects with varying degrees of glucose tolerance. Arteriosclerosis 1990; 10: 223-31.
Laakso M. Cardiovascular disease in type 2 diabetes: challenge for treatment and prevention. J Intern Med 2001; 249: 225-35.
Taskinen MR. Quantitative and qualitative lipoprotein abnormalities in diabetes mellitus. Diabetes 1992; 41: 12–7.
Pushparaj NP, Tan HKB, Tan HC. Th e mechanism of hypoglycemic action of the semi-purifi ed fractions of Averrhoa bilimbi in streptozotocin diabetic rats. Life Sciences 2001; 70: 535-547.
Mukherjee KP, Saha K, Pai M, Saha BP. Eff ect of Nelumbo nucifera rhizome extract on blood sugar level in rats. Journal of Ethnopharmacology 1997; 58: 207-213.