Jacek RECHULİCZ, Katarzyna OGNIK, R. Eugeniusz GRELA

The Effect of Adding Protein-Xanthophylls Concentrate (PX) from Lucerne (Medicago sativa) on Growth Parameters and Redox Profile in Muscles of carp, Cyprinus carpio (L.)

The effect of adding protein - xanthophyll from lucerne (PX) on growth parameters and the redox profile of carp muscle Cyp rinus carp io (L.) was studied. During a 10-week experiment, carp were kept in two types of tanks: Control, where fish were fed a commercial fish feed, and in experimental tanks (PX+), where the fish were fed the same fish feed with a 5% addition protein - xanthophyll from lucerne. Weekly for all fish the total length (Tl), standard length (Sl) and body weight (W) were measured. After experiment the coefficient of condition, specific growth rate (SGR) was calculated and with the spectrophotometric method oxidative status (MDA, SOD, Vit. C, CAT i H2O2) in carp muscle, was estimated. The results of observations have shown that the addition of 5% protein-xanthophyll concentrate (PX) from lucerne, to feed fish resulted in a greater increase (P>0.05) of total length and body weight of carp. Moreover, the addition of lucerne caused slightly better coefficients of condition and SGR. The addition of lucerne to fish feed affected the higher values of MDA (P<0.0001), SOD and CAT in the muscle of carp, at the same time the fish had a slightly lower value for content of Vit. C (P<0.0002) and H2O2. The addition of PX to carp feed induced an increase in products of the peroxidation lipid with simultaneous stimulation of the antioxidant enzymatic system in carp muscle.

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Al-Asgah, N.A. and Ali A. 1996. Effect of feeding different levels of wheat bran on the growth performance and body composition of Oreochromis niloticus. Agribiological Research, 49: 193-202.

Ali, A., Al-Asgah, N.A., Al-Ogaily, S.M. and Ali, S. 2003. Effect of feeding different levels of alfalfa meal on the growth performance and body composition of Nile tilapia (Oreochromis niloticus) fingerlings. Asian Fisheries Science, 16:59-67.

Bartosz, G. 2004. Second face of oxygen. PWN, Warszawa. 448 pp. (in Polish)

Ben Aziz, A., Grossman, S., Budowski, P., Ascarelli, I. and Bondi, A. 2006. Antioxidant properties of lucerne extracts. Journal of the Science of Food and Agriculture, 19(10): 605-608. doi: 10.1002/jsfa. 2740191011

Chatzifotis, S.G., Esteban, A. and Divanach, P. 2006. Fishmeal replacement by alfalfa protein concentrate in sharp snout sea bream Diplodus puntazzo. Fisheries Science, 72: 1313 1315. doi: 10.1111/j .1444-2906. 2006.01290.x

Czech, A., Ognik, K. and Grela, E. 2012. Efficacy of a mixture of synthetic antioxidant and protein- xanthophyll alfalfa concentrate in turkey hens feeding. Archivfür Geflgelkunde, 76(2): 105-112.

Dioundick, O.B. and Stom, D.I. 1990. Effect of dietary a- cellulose levels on the juvenile tilapia, Oreochromis mossambicus (Peters). Aquaculture, 91(3-4): 311- 315.doi: 10.1016/0044-8486(90)90196-T

Francis, G., Harinder P.S., Makkar, H.P.S. and Becker, K. 2002. Dietary supplementation with a Quilla j asaponin mixture improves growth performance and metabolic efficiency in common carp (Cyp rinus carp io L.). Aquaculture, 203(3-4): 311320. doi: 10.1016/S0044-8486(01)00628-7

Francis, G., Makkar, H.P.S. and Becker, K. 2002a. Effects of cyclic and regular feeding of Quillajasaponin supplemented diet on growth and metabolism of common carp (Cyp rinus carp io L.). Fish Physiology and Biochemistry, 24: 343350. doi: 10.1023/A: 1015047208108

Francis, G., Makkar, H.P.S. and Becker, K. 2001. Effects of Quillaj a saponins on growth, metabolism, egg production and muscle cholesterol in individually reared Nile tilapia (Oreochromis niloticus). Comparative Biochemistry and Physiology, 129C: 105 114. doi: 10.1016/S1532-0456(01)00189-2

Fu, X. 2003. Effect on plant leaf protein on lipotropy peroxidase system of rats. Chinese Journal of Veterinary and Science Technology, 11: 1-18.

Gaweł, E. and Grzelak, M. 2012.The effect of a protein- xanthophyll concentrate from alfalfa (phytobiotic) on animal production- acurrent review. Annals of Animal Science, 12(3): 281-289. doi: 10.2478/v10220-012- 0023-5

Gay, C. and Gębicki, J. M.2000. A critical evaluation of the effect of sorbitol on the ferric-xylenol orange hydroperoxide assay. Analytical Biochemistry, 284: 217-220. doi: 10.1006/abio.2000.4696

Greenwald, R.A. 1985. CRC handbook of methods for oxygen radical research. CRC Press Boca Raton, 447 pp.

Grela, E.R., Pietrzak, K., Kowalczuk-Vasilev, E., Klebaniuk, R. and Borucka, B. 2013. Analysis of chemical composition of protein-xanthophyll concentrate. Przemysł Chemiczny, 92(8): 1484-1488. (in Polish with English abstract)

Gutteridge, J.M. and Halliwell, B. 1990. The measurement and mechanism of lipid peroxidation in biological systems. Trends in Biochemical Sciences, 15: 129- 135. doi: 10.1016/0968-0004(90)90206-Q

Halver, J.E. and Hardy, R.W. 2002. Fish nutrition. Academic Press, 500 pp.

Jia, L., He, X. and Yang, Y. 1991.Evaluation of partial replacement of fish meal and soybean meal cake by alfalfa, Trif oliun sp., in practical diets for Chinese blunt snout bream, Megalobrama amblycephala fingerlings. In: S.S. De-Silva (Ed.), Proceedings of the 4th Fish Nutrition Research Workshop in Asia. No.5, Vijayawanda, India: 119-123.

Karwowska, M., Dolatowski, Z.J. and Grela, E.R. 2012. Influence of dietary supplementation with protein- xanthophylls (PX) concentrate of alfalfa investigations on pig meat quality. Fleisch Wirtschaft International, 27(1): 88-91.

Lovasova, E. and Sesztákova, E. 2009. Total antioxidant status a possible marker of environmental influences on animal organisms. Slovak Journal of Animal Science, 42(1): 42-45.

Lovell, T. (Ed.) 1998. Nutrition and feeding of fish. Springer, Kluwer Academic Publish, 267 pp.

Metwally, M.A.A. 2009. Effects of Garlic (Allium sativum) on some antioxidant activities in tilapia nilotica (Oreochromis niloticus).World Journal of Fish and Marine Sciences, 1(1): 56-64.

Mortensen, A. and Skibsted, L.H. 2000. Antioxidant activity of carotenoids in muscle food. In: E.A. Decker, C. Faustman and C.J. Lopez-Bote (Eds.), Antioxidants in muscle food. Nutritional strategies to improve quality, Wiley Interscience, New York: 357- 388.

Naylor, R.L., Goldburg, R.J., Primavera, J.H., Kautsky, N., Beveridge, M.C.M., Clay, J., Folke, C., Lubchenco, J., Mooney, H. and Troell, M. 2000. Effect of aquaculture on world fish supplies. Nature, 405: 1017- 1024. doi: 10.1038/35016500

Newall, C.A., Anderson, L.A. and Phillopson, J.D. 1996. Herbal medicines. A guide for health-care professionals. The Pharmaceutical Press, London, 296 pp.

Ognik, K., Patkowski, K. and Grela, E.R. 2012.Effect of a protein-xanthophyll concentrate from alfalfa and of genotype and sex of lambs on their blood redox profile. Bulletin of the Veterinary Institute in Pulawy, 56: 165-169. doi: 10.2478/v10213-012-0030-4

Olvera-Novoa, M.A., Campos, S.G., Sabido, M.G. and Martínez-Palacios, C.A. 1990.The use of alfalfa leaf protein concentrates as a protein source in diets for tilapia (Oreochromis mossambicus). Aquaculture, 90 (3-4): 291-302. doi: 10.1016/0044-8486(90)90253-J

Omaye, S.T., Tumbull, J.D. and Sauberlich, H.E. 1979. Selected methods for determination of ascorbic acid in animal cells, tissues and fluids. Methods in Enzymology, 62: 3-11. doi: 10.1016/0076-6879(79) 62181-X

Salih, M., Smith, D.M., Price, J.F. and Dawson, L.E. 1987. Modified extraction 2-thiobarbituric acid method for measuring lipid oxidation in poultry. Poultry Science, 66: 1483-1488. doi: 10.3382/ps.0661483

Xie, Z., Huang, J., Xu, X. and Jin, Z. 2008. Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate. Food Chemistry, 111: 370-376. doi: 10.1016/j .foodchem.2008.03.078

Yanar, M., Zeynep Erçen, Z., Hunt, A.Ö. and Büyükçapar, H.M. 2008. The use of alfalfa, Medicago sativa as a natural carotenoid source in diets of goldfish, Carassius auratus. Aquaculture, 284: 196200.doi: 10.1016/j .aquaculture.2008.07.050

Yousif, O.M., Alhadharmi, G. A. and Pessarakali, M. 1994. Evaluation of dehydrated alfalfa and saltbush (Artiplex) leaves in diets for tilapia (Orechromis aureus L.). Aquaculture, 126: 341-347. doi: 10.1016/0044-8486(94)90050-7